Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A4 F1 I2 R1 | 780 | 68.3 | 3343050 | 93.5% | 3125751 | 102.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913 | 4,606,094 | (G)8→11 | noncoding (72/87 nt) | leuV ← | tRNA‑Leu |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 4606086 | 9 (0.150) | 59 (0.910) +GGG |
40/182 | 0.3 | 86.6% | noncoding (80/87 nt) | leuV | tRNA‑Leu |
| ? | NC_000913 | 4606087 = | 9 (0.130) | noncoding (79/87 nt) | leuV | tRNA‑Leu | |||||
AGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/4605955‑4606086‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTG > NC_000913/4606087‑4606220 ||| AGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGG > 2:149037/1‑139 GTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGG < 2:762491/139‑1 TTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGG > 1:1370288/1‑139 TTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGG < 1:149037/139‑1 TTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCC < 2:632611/139‑1 CTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTC > 1:1262213/1‑139 CATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTA < 2:1635737/139‑1 TCGTGCCTGGCACTTATTGCTGGCATCATTCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAG < 2:1262213/139‑1 TCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAG < 2:526148/139‑1 GGCACTTATTGCCGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA > 2:857012/1‑139 GCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGACACTAAC > 2:1403848/1‑139 TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCT > 2:926815/1‑139 ATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGA > 2:917729/1‑139 GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAG > 2:372937/1‑139 GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCC > 1:1559718/1‑107 GGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCC < 2:1559718/107‑1 CATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG > 2:1418084/1‑139 CATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA > 2:346907/1‑124 CATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA < 1:346907/124‑1 GCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTA < 1:372937/139‑1 GATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCA < 1:926815/139‑1 ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA > 2:1466445/1‑139 ATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAA > 2:1253961/1‑139 TGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTA > 1:1108882/1‑103 TGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTA < 2:1108882/103‑1 ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC < 1:1466445/139‑1 ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC < 1:810764/139‑1 ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC < 2:1370288/139‑1 ATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGC < 1:1366667/139‑1 TTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCC > 1:1017513/1‑82 TTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCC < 2:1017513/82‑1 AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG > 2:827502/1‑114 AATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG < 1:827502/114‑1 CAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACA < 1:857012/139‑1 AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA < 1:41298/96‑1 AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA > 2:41298/1‑96 AGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAG < 1:1253961/139‑1 GGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAtacc > 2:318375/1‑135 GGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGA > 2:1011754/1‑68 GGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGA < 1:1011754/68‑1 TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA > 2:258980/1‑95 TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA < 1:258980/95‑1 TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTA < 1:518165/73‑1 TCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTA > 2:518165/1‑73 AAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTTAACCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTCACTctct > 2:834367/1‑135 AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACG > 2:266562/1‑64 AGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACG < 1:266562/64‑1 AGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTT < 2:483829/139‑1 GGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCA > 2:1412854/1‑46 GGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCA < 1:1412854/46‑1 GTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAtg < 1:278052/53‑3 GTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC > 1:1355122/1‑95 GTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACC < 2:1355122/95‑1 GTAAAGATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGG > 2:278052/1‑53 GATAATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGG < 1:917729/139‑1 AATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGG < 2:294004/139‑1 AATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGG < 1:1418084/139‑1 AATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA > 2:118493/1‑64 AATTGGTGCGA‑GGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTA < 1:118493/64‑1 cgaGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTG > 2:514266/4‑139 cgaGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTT < 2:601186/115‑1 cgaGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTT > 1:601186/4‑118 cgaGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAATTG > 2:701035/4‑139 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG < 1:281312/80‑1 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCG > 2:281312/2‑81 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG > 2:249756/2‑57 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCG < 1:249756/56‑1 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA > 1:28283/2‑42 aGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAA < 2:28283/41‑1 ||| AGTTGCGGCATTACATTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/4605955‑4606086‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTG > NC_000913/4606087‑4606220 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |