Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
771 |
44.8 |
2153074 |
94.1% |
2026042 |
109.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,495,050 |
C→T |
*81* (TAG→TAA) |
yfdY ← |
DUF2545 family putative inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,495,050 | 0 | C | T | 98.0%
| 154.2
/ ‑4.3
| 52 | *81* (TAG→TAA) | yfdY | DUF2545 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (28/22); total (29/23) |
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 |
CCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGC > NC_000913/2494923‑2495178
|
ccgccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGcc > 2:995111/1‑139 (MQ=255)
cgccCCCTTTTCCGACCAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa < 2:346934/139‑1 (MQ=255)
gccCCCTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCAt > 1:7979/1‑139 (MQ=255)
ccccTTTTCCGACAAAGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAc > 2:824137/1‑139 (MQ=255)
aaGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCg > 1:670661/1‑139 (MQ=255)
aGGCGAATCATCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGa > 2:148701/1‑139 (MQ=255)
aatcaTCTTATTCATCCTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAatcat > 2:184801/5‑139 (MQ=255)
ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCAAAAACCgg > 2:192094/1‑139 (MQ=255)
ccTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCAATACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCgg > 1:680688/1‑139 (MQ=255)
cTGTGCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCTCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCggg > 1:885879/1‑139 (MQ=255)
tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCa > 2:753760/1‑139 (MQ=255)
tgCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTATGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCAACAAGCCACAAGCCGGGGCa > 2:781972/1‑139 (MQ=255)
gCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa < 2:1005475/139‑1 (MQ=255)
gCGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCaa < 1:324605/139‑1 (MQ=255)
cGATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAg < 1:995111/139‑1 (MQ=255)
gATAACGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGa < 2:670661/139‑1 (MQ=255)
ataacGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa < 1:753760/135‑1 (MQ=255)
ataacGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa > 2:686392/5‑139 (MQ=255)
aacGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAAc < 1:686392/137‑1 (MQ=255)
acGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa < 1:929751/89‑1 (MQ=255)
acGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCa > 2:929751/2‑90 (MQ=255)
caTGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATccc < 2:663390/139‑1 (MQ=255)
cAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCa < 1:824137/139‑1 (MQ=255)
aGGTTAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAGATATCCCCGTCAc < 2:999147/139‑1 (MQ=255)
aGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCAc < 1:148701/139‑1 (MQ=255)
aGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCAc < 2:680688/139‑1 (MQ=255)
gTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTa > 2:885975/1‑139 (MQ=255)
cAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCa > 1:984416/1‑139 (MQ=255)
cATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTAc < 1:885975/139‑1 (MQ=255)
aTGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCg > 1:728724/1‑99 (MQ=255)
aTGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCg < 2:728724/99‑1 (MQ=255)
tGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCg > 2:643430/1‑139 (MQ=255)
tCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGcc > 1:354601/1‑139 (MQ=255)
tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa < 1:928309/100‑1 (MQ=255)
tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa > 2:928309/1‑100 (MQ=255)
tgctgatgTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTa > 2:112386/1‑139 (MQ=255)
gatgTGAACACCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACca > 1:37009/1‑139 (MQ=255)
cacCTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa < 1:112386/139‑1 (MQ=255)
cTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCAt < 1:592868/112‑1 (MQ=255)
cTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCAt > 2:592868/1‑112 (MQ=255)
cTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa < 2:113692/136‑1 (MQ=255)
cTGCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGa > 1:113692/1‑136 (MQ=255)
gCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAcc < 2:29942/41‑1 (MQ=255)
gCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATAcc > 1:29942/1‑41 (MQ=255)
gCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa < 2:548068/83‑1 (MQ=255)
gCCCAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGaa > 1:548068/1‑83 (MQ=255)
cccAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCAc > 2:74183/1‑101 (MQ=255)
cccAAATGAGCCATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCAc < 1:74183/101‑1 (MQ=255)
ccATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGc > 2:47185/1‑139 (MQ=255)
ccATCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGc > 2:316195/1‑139 (MQ=255)
tCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGc < 2:37009/139‑1 (MQ=255)
tCCTGACCCATTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGc > 1:512808/1‑139 (MQ=255)
|
CCGCCCCCTTTTCCGACAAAGGCGAATTATCTTATTCATCCTGTGCGATAGCGTCTTTCACATGGCAGGTGAACAATCATGTCTGATGCTGATGTGAACACCTGCCCAAATGAGCCATCCTGACCCACTACCGCCTGCCATACCCCACCATAAAGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGC > NC_000913/2494923‑2495178
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
