Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A8 F1 I1 R1 | 771 | 44.8 | 2153074 | 94.1% | 2026042 | 109.1 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,204,645 | G→A | *442* (TAG→TAA) N429N (AAC→AAT) |
zraR → purD ← |
fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,204,645 | 0 | G | A | 100.0% | 87.7 / NA | 28 | *442* (TAG→TAA) N429N (AAC→AAT) | zraR purD | fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/16); total (12/16) | |||||||||||
TTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCG > NC_000913/4204523‑4204768 | ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGa > 2:353011/1‑139 (MQ=255) gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGc > 2:253448/1‑139 (MQ=255) aaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat < 1:693183/139‑1 (MQ=255) gATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTc > 2:564173/1‑139 (MQ=255) cTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt < 1:158430/139‑1 (MQ=255) agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc < 1:57933/139‑1 (MQ=255) agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc < 1:253448/139‑1 (MQ=255) gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATc < 1:753158/139‑1 (MQ=255) caacaaAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg < 1:353011/139‑1 (MQ=255) aaaCCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCAt < 1:564173/139‑1 (MQ=255) gggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt < 1:474942/79‑1 (MQ=255) gggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt > 2:474942/1‑79 (MQ=255) gggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgc < 1:101097/128‑1 (MQ=255) gggATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgc > 2:101097/1‑128 (MQ=255) cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg > 1:780807/1‑104 (MQ=255) cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg < 2:678813/104‑1 (MQ=255) cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg < 2:780807/104‑1 (MQ=255) cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg > 1:678813/1‑104 (MQ=255) cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc > 1:391972/1‑139 (MQ=255) cGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa > 1:592350/1‑132 (MQ=255) cGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa < 2:592350/132‑1 (MQ=255) gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt > 2:328960/1‑70 (MQ=255) gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt < 1:328960/70‑1 (MQ=255) gCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg < 2:774873/139‑1 (MQ=255) aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat < 1:962042/45‑1 (MQ=255) aCTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGat > 2:962042/1‑45 (MQ=255) cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGcgcg > 1:599900/1‑131 (MQ=255) cgcgTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGcgcg < 2:599900/131‑1 (MQ=255) | TTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCG > NC_000913/4204523‑4204768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |