Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	771	44.8	2153074	94.1%	2026042	109.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,294	A→G	G489G (GGA→GGG)	metH →	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,294	0	A	G	100.0%	122.1 / NA	35	G489G (GGA→GGG)	metH	homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/17); total (18/17)

TATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG  >  NC_000913/4225168‑4225427

tATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACt                                                                                                                           >  1:465767/1‑139 (MQ=255)

        gCAAAGGCATTGTTAACGCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACg                                                                                                                   <  2:522150/139‑1 (MQ=255)

          aaaGGCATTGTTAACTCTATCTTGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGa                                                                                                                 >  2:1043751/1‑139 (MQ=255)

                    ttAACTCTATCTTGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGAt                                                                                                       <  1:1043751/139‑1 (MQ=255)

                            aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt                                                                                                >  1:1059052/1‑138 (MQ=255)

                            aTCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt                                                                                                <  2:1059052/138‑1 (MQ=255)

                             tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCg                                                                                              >  2:932393/1‑139 (MQ=255)

                              ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                             <  1:106453/139‑1 (MQ=255)

                                  aTGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGt                                                                                         >  1:404862/1‑139 (MQ=255)

                                  aTGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGt                                                                                         >  2:1020160/1‑139 (MQ=255)

                                     aaaGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACa                                                                                      <  1:932393/139‑1 (MQ=255)

                                            gCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCt                                                                               <  1:1020160/139‑1 (MQ=255)

                                             cGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc                                                                              >  2:946741/1‑139 (MQ=255)

                                             cGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc                                                                              >  2:484395/1‑139 (MQ=255)

                                               tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg                                                                                                                                    <  1:876926/83‑1 (MQ=255)

                                               tCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg                                                                                                                                    >  2:876926/1‑83 (MQ=255)

                                                cGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCAcc                                                                           >  1:545869/1‑139 (MQ=255)

                                                          aTCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTg                                                                 >  2:756809/1‑139 (MQ=255)

                                                                cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                           >  1:916102/1‑107 (MQ=255)

                                                                cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc                                                                                           <  2:916102/107‑1 (MQ=255)

                                                                cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                           <  2:404862/139‑1 (MQ=255)

                                                                cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc                                                           <  1:946741/139‑1 (MQ=255)

                                                                 aCGCGAAATTGTTGTGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                             >  2:399194/1‑104 (MQ=255)

                                                                 aCGCGAAATTGTTGTGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg                                                                                             <  1:399194/104‑1 (MQ=255)

                                                                       aaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCg                                                         >  1:590321/1‑134 (MQ=255)

                                                                       aaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCg                                                         <  2:590321/134‑1 (MQ=255)

                                                                          tgttgCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGTTTCCCGCCagaaga                                                 >  1:464512/1‑139 (MQ=255)

                                                                                               cAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagca                                                   <  2:645462/116‑3 (MQ=255)

                                                                                               cAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCagaa                                                   >  1:645462/1‑116 (MQ=255)

                                                                                                    gtggtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTcg                       <  1:756809/139‑1 (MQ=255)

                                                                                                             ggcTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCaa              >  2:644317/3‑139 (MQ=255)

                                                                                                              ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAAc             <  1:484395/139‑1 (MQ=255)

                                                                                                              ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAAc             >  2:61920/1‑139 (MQ=255)

                                                                                                                         cAGGGGCAGGCCGATACTCGCGCACGGATAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAag  <  1:644317/139‑1 (MQ=255)

                                                                                                                         cAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGTTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAag  <  2:464512/139‑1 (MQ=255)

TATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG  >  NC_000913/4225168‑4225427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A