Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	789,464	G→A	I172I (ATC→ATT)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	789,464	0	G	A	100.0%	109.7 / NA	35	I172I (ATC→ATT)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/15); total (20/15)

AGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACT  >  NC_000913/789340‑789598

aGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACa                                                                                                                          <  1:699552/139‑1 (MQ=255)

          gatgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAAc                                                                                                                <  2:1533815/139‑1 (MQ=255)

            tgatgaCGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTg                                                                                                              >  2:724764/1‑139 (MQ=255)

                  cgacAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTc                                                                                                        >  2:1539273/1‑139 (MQ=255)

                     cAGCCACACCTTT‑GGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCt                                                                                                    >  1:920566/1‑139 (MQ=255)

                          acacCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCt                                                                                                <  1:1539273/139‑1 (MQ=255)

                                    gCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAg                                                                                      >  2:897391/1‑139 (MQ=255)

                                        ggAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGcgcg                                                                                  >  1:561172/1‑139 (MQ=255)

                                                         ccAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAg                                                                 <  1:897391/139‑1 (MQ=255)

                                                               aGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATccc                                                                                                                                   >  1:1724523/1‑67 (MQ=255)

                                                               aGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATccc                                                                                                                                   <  2:1724523/67‑1 (MQ=255)

                                                               aGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAg                                                           >  2:405934/1‑139 (MQ=255)

                                                               aGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAg                                                           >  2:1297970/1‑139 (MQ=255)

                                                                     aaTCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCATCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATa                                                     >  2:1120556/1‑139 (MQ=255)

                                                                       tCGATCAGCAAGGCATGGTCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCt                                                                                                                      >  1:1206069/1‑72 (MQ=255)

                                                                       tCGATCAGCAAGGCATGGTCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCt                                                                                                                      <  2:1206069/72‑1 (MQ=255)

                                                                           tCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAgctg                                               >  2:248691/1‑139 (MQ=255)

                                                                                  ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTtgtg                                                                           <  1:387851/104‑1 (MQ=255)

                                                                                  ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTtgtg                                                                           >  2:387851/1‑104 (MQ=255)

                                                                                  ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCg                                                               >  1:790168/1‑116 (MQ=255)

                                                                                  ggCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCg                                                               <  2:790168/116‑1 (MQ=255)

                                                                                   gCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATa                                       <  2:561172/139‑1 (MQ=255)

                                                                                        aTCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGtt                                  >  1:1527694/1‑139 (MQ=255)

                                                                                            ttcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGctgctg                                            >  2:1715622/1‑125 (MQ=255)

                                                                                            ttcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGctgctg                                            <  1:1715622/125‑1 (MQ=255)

                                                                                             tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                            >  2:985133/1‑108 (MQ=255)

                                                                                             tcttGCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                            <  1:985133/108‑1 (MQ=255)

                                                                                                 gCCGAGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                         <  1:724764/139‑1 (MQ=255)

                                                                                                     aGCGCGGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACtt                     >  2:1839187/1‑139 (MQ=255)

                                                                                                       cgcgGAAATTAGCTGATCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTcc                   >  2:596735/1‑139 (MQ=255)

                                                                                                                      aTCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                            <  2:499667/83‑1 (MQ=255)

                                                                                                                      aTCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCa                                                            >  1:499667/1‑83 (MQ=255)

                                                                                                                      aTCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                         >  2:798515/1‑118 (MQ=255)

                                                                                                                      aTCCATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACcgcg                         <  1:798515/118‑1 (MQ=255)

                                                                                                                        ccATAATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGATCGCGACTTCCAGTGAAGCGGAAGAACt  <  1:1120556/139‑1 (MQ=255)

AGTTACTGTTGATGATGACGACAGCCACACCTTTGGGCATGGAAACTGCTTTGGTCCCCAGTGAGCGGCAATCGATCAGCAAGGCATGATCTTTCTTGCCGAGCGCGGAAATTAGCTGATCCATGATCCCGCAGTTACAGCCTACAAACTGGTTTTCTGCTTCCTGACCGTTAAGCGCGATTTGTGCGCCGTCCAGCGGCAGATGATAAAGCTGCTGCAATACGGTTCCGACCGCGACTTCCAGTGAAGCGGAAGAACT  >  NC_000913/789340‑789598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A