Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,896,932	G→A	M1M (GTG→ATG) †	sdaA →	L‑serine dehydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,896,932	0	G	A	100.0%	226.4 / NA	69	M1M (GTG→ATG) †	sdaA	L‑serine dehydratase 1
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (40/29); total (40/29)

ATTAGTTAAATCGCGGTTTTTGATTAGTTTAATTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGC  >  NC_000913/1896800‑1897063

atTAGTTAAATCGCGGTTTTTGATTAGTTTAATTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTa                                                                                                                               >  2:1848507/1‑139 (MQ=255)

  tAGTTAAATCGCGGTTTTTGATTAGTTTAATTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGt                                                                                                                             >  2:706822/1‑139 (MQ=255)

          tCGCGGTTTTTGATTAGTTTAATTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     <  1:706822/139‑1 (MQ=255)

                                    tgtgAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCt                                                                                           >  2:330966/1‑139 (MQ=255)

                                             ttAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGAc                                                                                                                    >  2:1569849/1‑105 (MQ=255)

                                             ttAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGAc                                                                                                                    <  1:1569849/105‑1 (MQ=255)

                                             ttAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTccc                                                                                  >  2:306990/1‑139 (MQ=255)

                                             ttAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTcat                                                                                  >  1:1452534/1‑137 (MQ=255)

                                              tAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCa                                                                                 <  1:1848507/139‑1 (MQ=255)

                                               aaGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     >  2:1512895/1‑102 (MQ=255)

                                               aaGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     <  1:1512895/102‑1 (MQ=255)

                                               aaGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTg                                                                                                        >  2:988418/1‑115 (MQ=255)

                                               aaGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTg                                                                                                        <  1:988418/115‑1 (MQ=255)

                                                  ccAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     <  2:832915/99‑1 (MQ=255)

                                                  ccAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     >  1:832915/1‑99 (MQ=255)

                                                  ccAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTc                                                                                              <  2:345294/122‑1 (MQ=255)

                                                  ccAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTc                                                                                              >  1:345294/1‑122 (MQ=255)

                                                  ccAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATAcc                                                                             >  1:856960/1‑139 (MQ=255)

                                                   cAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCg                                                                            <  2:1540464/139‑1 (MQ=255)

                                                     gTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGt                                                                           >  2:1301480/1‑138 (MQ=255)

                                                     gTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGt                                                                           <  1:1301480/138‑1 (MQ=255)

                                                     gTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTa                                                                          <  2:856960/139‑1 (MQ=255)

                                                     gTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTT‑‑CATACCGTAgg                                                                        <  2:1452534/139‑1 (MQ=255)

                                                      tCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTTCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCtcttct                                                                                      <  1:922038/126‑1 (MQ=255)

                                                      tCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAgg                                                                                                          >  2:295810/1‑106 (MQ=255)

                                                      tCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAgg                                                                                                          <  1:295810/106‑1 (MQ=255)

                                                      tCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCt                                                                                      >  2:922038/1‑126 (MQ=255)

                                                      tCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTACTTCGTTACTGGCAGTCCAGTCACCTTGTCAGGAGTATTATCATTATTAGTCGATTCGACATGTTTAATGTGGGGATTGGTCCCTCAGCTTCCCATACCGTAg                                                                         >  1:176137/1‑139 (MQ=255)

                                                                cccTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTa                                                                          <  1:1519419/128‑1 (MQ=255)

                                                                cccTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTa                                                                          >  2:1519419/1‑128 (MQ=255)

                                                                  ctctTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAgg                                                             <  2:1526846/139‑1 (MQ=255)

                                                                             tGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAg                                                  >  1:1476259/1‑139 (MQ=255)

                                                                             tGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAg                                                  <  1:330966/139‑1 (MQ=255)

                                                                             tGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAg                                                  >  1:1608415/1‑139 (MQ=255)

                                                                             tGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAg                                                  >  1:1021585/1‑139 (MQ=255)

                                                                               cgcTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCACATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                >  1:1824461/1‑139 (MQ=255)

                                                                                  tGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATc                                                                                       <  1:1521606/97‑1 (MQ=255)

                                                                                  tGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATc                                                                                       >  2:1521606/1‑97 (MQ=255)

                                                                                   gTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCAt                                                                                <  1:1134439/103‑1 (MQ=255)

                                                                                   gTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCAt                                                                                >  2:1134439/1‑103 (MQ=255)

                                                                                       ttaGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCgatg                                        >  1:433662/1‑139 (MQ=255)

                                                                                         aGTTCGTTACTGGAAGTGCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     <  2:1226053/60‑1 (MQ=255)

                                                                                         aGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     >  1:1226053/1‑60 (MQ=255)

                                                                                         aGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCgatgat                                      >  1:1428561/1‑139 (MQ=255)

                                                                                              gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATg                                                                 <  1:1631005/107‑1 (MQ=255)

                                                                                              gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATg                                                                 >  2:1631005/1‑107 (MQ=255)

                                                                                              gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                <  2:533405/124‑1 (MQ=255)

                                                                                              gTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                >  1:533405/1‑124 (MQ=255)

                                                                                               ttACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                >  1:628750/1‑123 (MQ=255)

                                                                                               ttACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                <  2:628750/123‑1 (MQ=255)

                                                                                                 aCTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                <  2:958516/121‑1 (MQ=255)

                                                                                                 aCTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGtt                                                >  1:958516/1‑121 (MQ=255)

                                                                                                      aaGTCCAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAg                         <  2:1428561/139‑1 (MQ=255)

                                                                                                          ccAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCa                                                                                 <  1:1466269/79‑1 (MQ=255)

                                                                                                          ccAGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCa                                                                                 >  2:1466269/1‑79 (MQ=255)

                                                                                                            aGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCt                                                                                      >  2:1745331/1‑72 (MQ=255)

                                                                                                            aGTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCt                                                                                      <  1:1745331/72‑1 (MQ=255)

                                                                                                             gTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGAt                                                                                                   >  1:554106/1‑58 (MQ=255)

                                                                                                             gTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGAt                                                                                                   <  2:554106/58‑1 (MQ=255)

                                                                                                             gTCACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACt                  >  1:49516/1‑139 (MQ=255)

                                                                                                               cACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     <  2:1312462/38‑1 (MQ=255)

                                                                                                               cACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGa                                                                                                                     >  1:1312462/1‑38 (MQ=255)

                                                                                                               cACCTTGTCAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTgg                >  1:900730/1‑139 (MQ=255)

                                                                                                                       cAGGAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGtt        >  1:622394/1‑139 (MQ=255)

                                                                                                                          gAGTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACt     >  2:1663776/1‑139 (MQ=255)

                                                                                                                            gTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAg                                                                         >  1:180128/1‑69 (MQ=255)

                                                                                                                            gTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAg                                                                         <  2:180128/69‑1 (MQ=255)

                                                                                                                            gTATTATCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTcg   >  1:1589551/1‑139 (MQ=255)

                                                                                                                             tattatCATGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTcgc  >  2:54234/1‑139 (MQ=255)

ATTAGTTAAATCGCGGTTTTTGATTAGTTTAATTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTCACCTTGTCAGGAGTATTATCGTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCAGGTAAACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGC  >  NC_000913/1896800‑1897063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A