Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F1 I2 R1 772 77.0 3769570 91.6% 3452926 105.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,645,013 C→T *771* (TAG→TAA)  pbpC ← penicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,645,0130CT100.0% 208.2 / NA 64*771* (TAG→TAA) pbpCpenicillin‑insensitive murein repair transglycosylase, inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/32);  total (32/32)

TTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NC_000913/2644883‑2645148
                                                                                                                                  |                                                                                                                                       
ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCAt                                                                                                                                 >  1:1781064/1‑139 (MQ=255)
ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCAt                                                                                                                                 >  1:1177097/1‑139 (MQ=255)
ttCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTACTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCAt                                                                                                                                 >  2:1821451/1‑139 (MQ=255)
 tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATg                                                                                                                                <  2:1177097/139‑1 (MQ=255)
     ttgttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACaa                                                                                                                            >  1:1239196/1‑139 (MQ=255)
        ttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAAtt                                                                                                                         >  2:664963/1‑139 (MQ=255)
                     cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  2:1781064/139‑1 (MQ=255)
                     cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  2:137912/139‑1 (MQ=255)
                     cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            >  2:380817/1‑139 (MQ=255)
                         cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           <  1:115013/136‑1 (MQ=255)
                         cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:115013/1‑136 (MQ=255)
                                 cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTc                                                                                                >  1:1120235/1‑139 (MQ=255)
                                 cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTc                                                                                                >  1:961200/1‑139 (MQ=255)
                                   cgGATTATAATTAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcat                                                                                              <  2:1221739/139‑1 (MQ=255)
                                               aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCa                                                                                  <  1:380817/139‑1 (MQ=255)
                                                 cAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAATATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                <  2:1120235/139‑1 (MQ=255)
                                                 cAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  2:369580/1‑139 (MQ=255)
                                                 cAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                <  2:961200/139‑1 (MQ=255)
                                                 cAACAGGGCCGTTGACTACTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                <  1:1821451/139‑1 (MQ=255)
                                                    cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  1:176754/108‑1 (MQ=255)
                                                    cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            >  2:176754/1‑108 (MQ=255)
                                                    cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGa                                                                             >  1:1387583/1‑139 (MQ=255)
                                                       ggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  2:1239196/139‑1 (MQ=255)
                                                         ccGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATc                                                                        >  1:1154112/1‑139 (MQ=255)
                                                                 tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                <  1:664963/139‑1 (MQ=255)
                                                                   ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACa                                                                                                                             >  2:1343262/1‑76 (MQ=255)
                                                                   ttGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACa                                                                                                                             <  1:1343262/76‑1 (MQ=255)
                                                                         aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAg                                                                                    <  1:151319/111‑1 (MQ=255)
                                                                         aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAg                                                                                    >  2:151319/1‑111 (MQ=255)
                                                                         aaGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCaaa                                                        >  1:409798/1‑139 (MQ=255)
                                                                           ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg                                                      >  1:551033/1‑139 (MQ=255)
                                                                             tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatccatc                                                                                         <  1:1507843/102‑1 (MQ=255)
                                                                             tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatccatc                                                                                         >  2:1507843/1‑102 (MQ=255)
                                                                              aaCATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg                                                   >  1:1041074/1‑139 (MQ=255)
                                                                                cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                            >  2:1537815/1‑128 (MQ=255)
                                                                                cATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                            <  1:1537815/128‑1 (MQ=255)
                                                                                 aTTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGt                                                <  2:551033/139‑1 (MQ=255)
                                                                                  tttttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTc                                               <  1:369580/139‑1 (MQ=255)
                                                                                     ttttATAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                            <  2:1105323/123‑1 (MQ=255)
                                                                                     ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                <  1:414381/103‑1 (MQ=255)
                                                                                     ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  2:414381/1‑103 (MQ=255)
                                                                                     ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                >  1:225008/1‑119 (MQ=255)
                                                                                     ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                <  2:225008/119‑1 (MQ=255)
                                                                                     ttttAAAATAAAATGAGTTTCAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGt                                                            >  1:1105323/1‑123 (MQ=255)
                                                                                                 atGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:1041074/139‑1 (MQ=255)
                                                                                                 atGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTCCGCCCGCGt                                <  2:1261813/139‑1 (MQ=255)
                                                                                                              acaaTCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatccatc                                                                                         >  2:1071719/1‑69 (MQ=255)
                                                                                                              acaaTCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatccatc                                                                                         <  1:1071719/69‑1 (MQ=255)
                                                                                                              acaaTCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatc                                                                                             <  1:546823/65‑1 (MQ=255)
                                                                                                              acaaTCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTcatc                                                                                             >  2:546823/1‑65 (MQ=255)
                                                                                                                     gggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAcc                                                                                      >  2:1572365/1‑65 (MQ=255)
                                                                                                                     gggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAcc                                                                                      <  1:1572365/65‑1 (MQ=255)
                                                                                                                      ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          >  2:1428130/1‑76 (MQ=255)
                                                                                                                      ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  1:1428130/76‑1 (MQ=255)
                                                                                                                      ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                           >  2:27138/1‑91 (MQ=255)
                                                                                                                      ggACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                           <  1:27138/91‑1 (MQ=255)
                                                                                                                         cAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGa        >  2:724215/1‑139 (MQ=255)
                                                                                                                          aaaaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 <  1:880907/49‑1 (MQ=255)
                                                                                                                          aaaaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 >  2:880907/1‑49 (MQ=255)
                                                                                                                           aaaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAc                                                                                       >  1:93133/1‑58 (MQ=255)
                                                                                                                           aaaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAc                                                                                       <  2:93133/58‑1 (MQ=255)
                                                                                                                            aaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCcgc                                  >  2:1534041/1‑110 (MQ=255)
                                                                                                                            aaCAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCcgc                                  <  1:1534041/110‑1 (MQ=255)
                                                                                                                               aGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAaccac  <  2:409798/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
TTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NC_000913/2644883‑2645148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A