Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
772 |
77.0 |
3769570 |
91.6% |
3452926 |
105.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,737,495 |
G→A |
*114* (TAG→TAA) |
raiA → |
cold shock protein associated with 30S ribosomal subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,737,495 | 0 | G | A | 100.0%
| 111.1
/ NA
| 35 | *114* (TAG→TAA) | raiA | cold shock protein associated with 30S ribosomal subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/18); total (17/18) |
TGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCAT > NC_000913/2737362‑2737626
|
tGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCtt < 1:1690828/139‑1 (MQ=255)
cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg > 1:727809/1‑139 (MQ=255)
cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg < 1:1389814/139‑1 (MQ=255)
cGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTACGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg > 2:260007/1‑139 (MQ=255)
caaGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCtt > 2:1137004/1‑139 (MQ=255)
tGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg > 1:105496/1‑113 (MQ=255)
tGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTg < 2:105496/113‑1 (MQ=255)
tGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAAc > 1:832969/1‑127 (MQ=255)
tGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAAc < 2:832969/127‑1 (MQ=255)
tGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgc > 2:1562090/1‑139 (MQ=255)
aCGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt < 1:1137004/139‑1 (MQ=255)
cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttttt > 1:466553/1‑139 (MQ=255)
cAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGttttt > 1:1018130/1‑139 (MQ=255)
tGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTttgtt < 1:163982/130‑1 (MQ=255)
tGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTttgtt > 2:163982/1‑130 (MQ=255)
cagcaCAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGaa < 1:1600060/139‑1 (MQ=255)
aacacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGaaa > 2:512416/3‑139 (MQ=255)
cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATc < 2:951591/95‑1 (MQ=255)
cacaAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATc > 1:951591/1‑95 (MQ=255)
aGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTAACAGCGTGAAAACAGTAc < 1:1041704/139‑1 (MQ=255)
aGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTACGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTAc < 1:260007/139‑1 (MQ=255)
gCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATcc > 2:1589580/1‑71 (MQ=255)
gCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATcc < 1:1589580/71‑1 (MQ=255)
aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta > 1:595966/1‑139 (MQ=255)
aaGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta < 2:1633230/139‑1 (MQ=255)
tcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt < 2:1245120/100‑1 (MQ=255)
tcgtGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGt > 1:1245120/1‑100 (MQ=255)
gAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGaaac < 1:512416/139‑1 (MQ=255)
aaaGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGaaaca < 2:727809/139‑1 (MQ=255)
ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAg > 2:1236077/1‑98 (MQ=255)
ccAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAg < 1:1236077/98‑1 (MQ=255)
cAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta > 1:1644315/1‑105 (MQ=255)
cAACTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGgta < 2:1644315/105‑1 (MQ=255)
aCTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcgc < 2:1396674/69‑1 (MQ=255)
aCTTCGTCGAAGAAGTTGAAGAAGAGTAATCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGgcgc > 1:1396674/1‑69 (MQ=255)
agtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCAt > 2:562803/1‑136 (MQ=255)
agtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCAt < 1:562803/136‑1 (MQ=255)
gtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACggg > 2:1265760/1‑76 (MQ=255)
gtaatCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACggg < 1:1265760/76‑1 (MQ=255)
|
TGTACACCGCAATTAACGAATTGATCAACAAGCTGGAACGGCAGCTCAATAAACTGCAGCACAAAGGCGAAGCACGTCGTGCCGCAACATCGGTGAAAGACGCCAACTTCGTCGAAGAAGTTGAAGAAGAGTAGTCCTTTATATTGAGTGTATCGCCAACGCGCCTTCGGGCGCGTTTTTTGTTGACAGCGTGAAAACAGTACGGGTACTGTACTAAAGTCACTTAAGGAAACAAACATGAAACACATACCGTTTTTCTTCGCAT > NC_000913/2737362‑2737626
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
