Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	772	77.0	3769570	91.6%	3452926	105.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	279 (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	270.2 / NA	83	279 (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (41/42); total (41/42)

ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGC  >  NC_000913/2983154‑2983423

acaGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAgc                                                                                                                                     >  1:590073/1‑139 (MQ=255)

  aGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAgcgc                                                                                                                                   <  1:893894/139‑1 (MQ=255)

    cccGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCaa                                                                                                                                 >  1:1496121/1‑139 (MQ=255)

          tGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCttt                                                                                                                           <  2:1177784/139‑1 (MQ=255)

               cAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACgg                                                                                                                      <  2:1320991/139‑1 (MQ=255)

                 gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGcc                                                                                                                    <  2:1769976/139‑1 (MQ=255)

                    ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:1496121/139‑1 (MQ=255)

                    ccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:645547/139‑1 (MQ=255)

                    ccAACGCCATCCCCTGACCCAGTCCAGTATCACAAACAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  1:203973/139‑1 (MQ=255)

                       aCGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATgg                                                                                                              >  1:1882247/1‑139 (MQ=255)

                          ccATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTcc                                                                                                           <  2:590073/139‑1 (MQ=255)

                          ccAGCCCCTGACCAAGTCCAGTATCACAACCAGTGACGACCTCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTcc                                                                                                           <  1:1315433/139‑1 (MQ=255)

                                         gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCttt                                                                                                                           <  1:990383/108‑1 (MQ=255)

                                         gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCttt                                                                                                                           >  2:990383/1‑108 (MQ=255)

                                         gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAcc                                                                                            >  1:1121434/1‑139 (MQ=255)

                                          tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAgcg                                                                                                                                    <  1:925346/98‑1 (MQ=255)

                                          tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAgcg                                                                                                                                    >  2:925346/1‑98 (MQ=255)

                                               tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACg                                                                                                                       >  2:1358064/1‑106 (MQ=255)

                                               tATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACg                                                                                                                       <  1:1358064/106‑1 (MQ=255)

                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGt                                                                                        <  1:1070165/132‑1 (MQ=255)

                                                    caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGt                                                                                        >  2:1070165/1‑132 (MQ=255)

                                                      aCCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                       <  2:885601/115‑1 (MQ=255)

                                                      aCCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                       >  1:885601/1‑115 (MQ=255)

                                                        cAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                             <  2:431283/139‑1 (MQ=255)

                                                          gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                  <  2:806186/100‑1 (MQ=255)

                                                          gTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                  >  1:806186/1‑100 (MQ=255)

                                                           tGACGACCGCAACTTTACCTTCGAGAGAAGATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                            >  1:1577440/1‑139 (MQ=255)

                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                             >  2:862746/1‑120 (MQ=255)

                                                           tGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                             <  1:862746/120‑1 (MQ=255)

                                                             acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                        <  1:1589567/107‑1 (MQ=255)

                                                             acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCata                                                                                                        >  2:1589567/1‑107 (MQ=255)

                                                                     aaCTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:317773/90‑1 (MQ=255)

                                                                     aaCTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 >  1:317773/1‑90 (MQ=255)

                                                                     aaCTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATaa                                                                >  1:705863/1‑139 (MQ=255)

                                                                        tttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAgg                                                             <  2:1562060/139‑1 (MQ=255)

                                                                         ttACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGt                                                            <  2:76919/139‑1 (MQ=255)

                                                                           aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 >  1:809049/1‑84 (MQ=255)

                                                                           aCCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:809049/84‑1 (MQ=255)

                                                                                 gagggaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                             >  2:239905/5‑98 (MQ=255)

                                                                                 gagggaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                             <  1:239905/98‑1 (MQ=255)

                                                                                 gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:537534/78‑1 (MQ=255)

                                                                                 gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 >  1:537534/1‑78 (MQ=255)

                                                                                 gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑‑‑CAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTg                                                  >  2:570997/1‑139 (MQ=255)

                                                                                  agagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCttt                                                   >  1:1313288/1‑139 (MQ=255)

                                                                                  agagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCttt                                                   >  2:36998/1‑139 (MQ=255)

                                                                                            cACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                         <  1:1418659/139‑1 (MQ=255)

                                                                                               ttAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             >  2:1090794/1‑132 (MQ=255)

                                                                                               ttAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             <  1:1090794/132‑1 (MQ=255)

                                                                                                tAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCgg                                     >  1:1068241/1‑139 (MQ=255)

                                                                                                        tAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                             <  1:1689380/91‑1 (MQ=255)

                                                                                                        tAACAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                             >  2:1689380/1‑91 (MQ=255)

                                                                                                         aaCAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             >  1:814025/1‑122 (MQ=255)

                                                                                                         aaCAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             <  2:814025/122‑1 (MQ=255)

                                                                                                         aaCAATACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCg                            >  1:1352234/1‑139 (MQ=255)

                                                                                                              tACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 >  1:564958/1‑49 (MQ=255)

                                                                                                              tACCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                                 <  2:564958/49‑1 (MQ=255)

                                                                                                               aCCTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAATGATCTGGTTTTCACCGAACATGCCCCAGATAAAGGAATAAGCTTTGGTTCCGACACCGGAATGAATCGACCAGc                      >  1:1397676/1‑139 (MQ=255)

                                                                                                                 cTTATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTc                    >  1:69542/1‑139 (MQ=255)

                                                                                                                   tATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                                  <  2:1292756/75‑1 (MQ=255)

                                                                                                                   tATTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                                  >  1:1292756/1‑75 (MQ=255)

                                                                                                                   tATTCGTTTATGCC‑‑‑‑CAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                <  1:570997/139‑1 (MQ=255)

                                                                                                                    aTTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                           <  2:17221/129‑1 (MQ=255)

                                                                                                                    aTTCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                           >  1:17221/1‑129 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                                  <  2:384153/73‑1 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCAc                                                                                  >  1:384153/1‑73 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                <  2:1882247/139‑1 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                >  1:1018946/1‑139 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                >  2:1631483/1‑139 (MQ=255)

                                                                                                                     ttCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                <  2:1018946/139‑1 (MQ=255)

                                                                                                                      tCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                >  2:1831993/1‑138 (MQ=255)

                                                                                                                      tCGTTTATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCgggg                <  1:1831993/138‑1 (MQ=255)

                                                                                                                          ttATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAc                                                                              <  1:1113137/72‑1 (MQ=255)

                                                                                                                          ttATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAc                                                                              >  2:1113137/1‑72 (MQ=255)

                                                                                                                          ttATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCTCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                           <  2:1752830/123‑1 (MQ=255)

                                                                                                                          ttATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCTCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                           >  1:1752830/1‑123 (MQ=255)

                                                                                                                           tATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                            >  1:153484/1‑73 (MQ=255)

                                                                                                                           tATGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                            <  2:153484/73‑1 (MQ=255)

                                                                                                                            aTGCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCAc         <  2:1313288/139‑1 (MQ=255)

                                                                                                                              gCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                        >  1:1502909/1‑74 (MQ=255)

                                                                                                                              gCC‑‑CACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                        <  2:1502909/74‑1 (MQ=255)

                                                                                                                                c‑‑cACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGcc     <  2:69542/139‑1 (MQ=255)

                                                                                                                                     caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTcc                                             >  2:984452/1‑96 (MQ=255)

                                                                                                                                     caATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGc  >  1:245757/1‑139 (MQ=255)

ACAGCCCGCTTGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCC‑‑CACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCTGC  >  NC_000913/2983154‑2983423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A