Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F1 I1 R1
|
759 |
71.0 |
3673240 |
91.5% |
3361014 |
104.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
375,307 |
C→T |
intergenic (+426/‑152) |
mhpE → / → mhpT |
4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 375,307 | 0 | C | T | 100.0%
| 152.5
/ NA
| 49 | intergenic (+426/‑152) | mhpE/mhpT | 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/21); total (28/21) |
GCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTC > NC_000913/375171‑375441
|
gcgcGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAgtgt < 1:1016111/139‑1 (MQ=37)
aaTTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCATAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCa < 2:177435/139‑1 (MQ=255)
ccTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGca > 2:801922/1‑139 (MQ=21)
gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCacac > 2:1309861/1‑139 (MQ=25)
gATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCacac > 1:167702/1‑139 (MQ=25)
cgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCAt < 1:800296/124‑1 (MQ=18)
cgcgTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCAt > 2:800296/1‑124 (MQ=17)
ttAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGAt > 2:1151825/1‑139 (MQ=25)
cATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAAc > 2:127485/1‑139 (MQ=255)
tGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGTATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCgg > 1:1100328/1‑139 (MQ=255)
cgcACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGcc > 2:124087/1‑139 (MQ=255)
cTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACAttt > 1:1530017/1‑139 (MQ=255)
ccGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGcaca > 2:1192806/1‑95 (MQ=255)
ccGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGcaca < 1:1192806/95‑1 (MQ=255)
ccGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATATCGc > 2:1208834/1‑139 (MQ=255)
tGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATa > 2:917991/1‑108 (MQ=255)
tGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATa < 1:917991/108‑1 (MQ=255)
tGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTa < 1:801922/139‑1 (MQ=255)
cggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGc > 1:434887/1‑72 (MQ=25)
cggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGc < 2:434887/72‑1 (MQ=255)
cggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAgg > 1:1711352/1‑139 (MQ=255)
ggggttaaaggcTTTTCCGGGCTACGGGATGGGGCGGGATTTGGTAGG‑CTGGTTAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:358601/128‑1 (MQ=18)
ggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:1151825/139‑1 (MQ=255)
ggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:857824/139‑1 (MQ=255)
ggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:1309861/139‑1 (MQ=255)
ggcgTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 2:1530017/139‑1 (MQ=255)
ttATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc > 1:1146192/1‑127 (MQ=255)
ttATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 2:1146192/127‑1 (MQ=255)
aTCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGt > 2:100594/1‑139 (MQ=255)
c‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:629175/115‑1 (MQ=255)
c‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc > 2:629175/1‑115 (MQ=255)
ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCt > 1:706517/1‑139 (MQ=255)
gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTg > 1:670924/1‑139 (MQ=255)
cgGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGTATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa < 2:1767083/116‑1 (MQ=255)
cgGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:1432924/106‑1 (MQ=255)
cgGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc > 2:1432924/1‑106 (MQ=255)
cgGGAATTTGTAGGCCTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTaaa > 1:1767083/1‑116 (MQ=255)
cTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGAt < 2:199049/51‑1 (MQ=255)
cTGATAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGAt > 1:199049/1‑51 (MQ=255)
aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATg > 2:1490618/1‑49 (MQ=255)
aTAAGACGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATg < 1:1490618/49‑1 (MQ=255)
aCGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCAtt < 2:711028/90‑1 (MQ=255)
aCGCGTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCAtt > 1:711028/1‑90 (MQ=255)
cgcgTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGc > 2:1020870/1‑139 (MQ=255)
gTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc > 2:1235712/1‑79 (MQ=255)
gTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGc < 1:1235712/79‑1 (MQ=255)
gTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACt < 2:167702/139‑1 (MQ=255)
gTTAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACt < 2:706517/139‑1 (MQ=255)
ttAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTc > 2:353208/1‑139 (MQ=255)
ttAGTGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTc > 2:373361/1‑139 (MQ=255)
|
GCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC‑GGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCACACGACTGCCGGATGCGATAAACGTCTTGTCCGGCCTACATTTCGCCCGTAGGCAGTCATTAAATAGTTCTGATTACGCGGCTGGCAATGTATCAGTCGCGATTCACCTGCACTC > NC_000913/375171‑375441
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
