Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,297	C→T	395 (TAG→TAA)	araJ ←	L‑arabinose‑inducible putative transporter, MFS family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,297	0	C	T	100.0%	117.6 / NA	37	395 (TAG→TAA)	araJ	L‑arabinose‑inducible putative transporter, MFS family
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/19); total (18/19)

AATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTAT  >  NC_000913/411165‑411421

aaTAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTAcccc                                                                                                                        <  1:179818/139‑1 (MQ=255)

 aTAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                       <  2:975827/139‑1 (MQ=255)

            gATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGcc                                                                                                            <  2:714692/139‑1 (MQ=255)

             aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCa                                                                                                           >  2:31532/1‑139 (MQ=255)

               gCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGc                                                                                                         <  2:1796407/139‑1 (MQ=255)

                   gATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCg                                                                                                     >  1:11711/1‑139 (MQ=255)

                   gATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCg                                                                                                     <  2:11711/139‑1 (MQ=255)

                               gCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAATCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTAt                                                                                               <  1:148053/133‑1 (MQ=255)

                               gCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAATCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTAt                                                                                               >  2:148053/1‑133 (MQ=255)

                                    gTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCGGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGt                                                                                                 <  1:1070845/126‑1 (MQ=255)

                                    gTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGt                                                                                                 >  2:1070845/1‑126 (MQ=255)

                                                   cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCg                                                                                                              >  1:460152/1‑98 (MQ=255)

                                                   cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCg                                                                                                              <  2:460152/98‑1 (MQ=255)

                                                   cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCt                                                                                   <  1:420704/125‑1 (MQ=255)

                                                   cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCt                                                                                   >  2:420704/1‑125 (MQ=255)

                                                          tgCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTg                                                                                                                    >  1:1492805/1‑85 (MQ=255)

                                                          tgCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTg                                                                                                                    <  2:1492805/85‑1 (MQ=255)

                                                             cAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATAcagca                                                           <  2:1695100/139‑1 (MQ=255)

                                                                     cgTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAacgac                                                   >  2:1392296/1‑139 (MQ=255)

                                                                               aTAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                       <  2:623179/109‑1 (MQ=255)

                                                                               aTAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                       >  1:623179/1‑109 (MQ=255)

                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCAcc                                                                                                      <  2:498162/68‑1 (MQ=255)

                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCAcc                                                                                                      >  1:498162/1‑68 (MQ=255)

                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAg                                                                                                  <  1:1621565/72‑1 (MQ=255)

                                                                                         cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAg                                                                                                  >  2:1621565/1‑72 (MQ=255)

                                                                                              cgcATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                             >  1:1801967/1‑136 (MQ=255)

                                                                                              cgcATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCa                             <  2:1801967/136‑1 (MQ=255)

                                                                                                 aTCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCg                                                                                                     >  1:280664/1‑61 (MQ=255)

                                                                                                 aTCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCg                                                                                                     <  2:280664/61‑1 (MQ=255)

                                                                                                    cGGCAATAAGTAATGAGAACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                       >  1:553520/1‑88 (MQ=255)

                                                                                                    cGGCAATAAGTAATGAGAACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                       <  2:553520/88‑1 (MQ=255)

                                                                                                           aaGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcgc             <  1:477659/139‑1 (MQ=255)

                                                                                                           aaGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcgc             <  2:172036/139‑1 (MQ=255)

                                                                                                                aTGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGt        >  1:1197000/1‑139 (MQ=255)

                                                                                                                     cACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATta   >  2:1762720/1‑139 (MQ=255)

                                                                                                                      aCCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtat  >  2:1437151/1‑139 (MQ=255)

                                                                                                                                 aaCTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCaaa                                     >  2:351570/1‑93 (MQ=255)

AATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTAT  >  NC_000913/411165‑411421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A