Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F1 I1 R1	759	71.0	3673240	91.5%	3361014	104.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,233,597	G→A	240 (TAG→TAA)	sanA →	DUF218 superfamily vancomycin high temperature exclusion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,233,597	0	G	A	100.0%	102.7 / NA	33	240 (TAG→TAA)	sanA	DUF218 superfamily vancomycin high temperature exclusion protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/17); total (16/17)

TTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGT  >  NC_000913/2233462‑2233727

ttttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTa                                                                                                                                 >  1:934857/1‑139 (MQ=255)

 tttAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTAt                                                                                                                                <  1:1478288/139‑1 (MQ=255)

         tGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAc                                                                                                                        >  1:186725/1‑139 (MQ=255)

                 gTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGttt                                                                                                                >  2:1277054/1‑139 (MQ=255)

                           gCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTg                                                                                                      <  2:934857/139‑1 (MQ=255)

                           gCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTg                                                                                                      <  2:186725/139‑1 (MQ=255)

                               cTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAA‑TACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGtt                                                                                                 >  1:523935/1‑139 (MQ=255)

                                               cTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCg                                                                                  <  1:1277054/139‑1 (MQ=255)

                                                 aTGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGat                                                                                >  2:1239741/1‑139 (MQ=255)

                                                              cGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc                                                                   >  2:1602974/1‑139 (MQ=255)

                                                              cGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgc                                                                   >  1:1686222/1‑139 (MQ=255)

                                                              cGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAA‑TACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCcgcg                                                                  <  2:523935/139‑1 (MQ=255)

                                                                gAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTGACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGa                                                                 <  1:1155399/139‑1 (MQ=255)

                                                                     tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                      >  1:926254/1‑81 (MQ=255)

                                                                     tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                      <  2:926254/81‑1 (MQ=255)

                                                                               tATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTg                                                  <  1:359741/139‑1 (MQ=255)

                                                                                 tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                      >  1:1315773/1‑69 (MQ=255)

                                                                                 tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTAcag                                                                                                                      <  2:1315773/69‑1 (MQ=255)

                                                                                        gTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAgg                                         >  2:509858/1‑139 (MQ=255)

                                                                                           acacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGgcgc                                      >  2:602137/1‑139 (MQ=255)

                                                                                            cacCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGcgcg                                     <  1:1002960/139‑1 (MQ=255)

                                                                                                          cTTGAATTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGt                       <  2:1686222/139‑1 (MQ=255)

                                                                                                              aaTTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                     >  1:1557200/1‑73 (MQ=255)

                                                                                                              aaTTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATc                                                                                     <  2:1557200/73‑1 (MQ=255)

                                                                                                               aTTACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTaaaa                  >  2:807357/1‑139 (MQ=255)

                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGAt                                   <  2:483466/120‑1 (MQ=255)

                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGAt                                   >  1:483466/1‑120 (MQ=255)

                                                                                                                 tACAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAAt                >  2:295087/1‑139 (MQ=255)

                                                                                                                   cAAAAGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGc              >  1:405954/1‑139 (MQ=255)

                                                                                                                     aaaGAAACAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAc            <  1:602137/139‑1 (MQ=255)

                                                                                                                           aCAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc      <  1:509858/139‑1 (MQ=255)

                                                                                                                            cAAGGAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAcc     <  2:1459416/139‑1 (MQ=255)

                                                                                                                               ggAAAGTAATTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGgt  <  2:405954/139‑1 (MQ=255)

TTTTAAACGTGAACCGCGTTTTTTAGGGCCGCTGGTCCCTATTCCGGCTATGCACCAGGTACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGT  >  NC_000913/2233462‑2233727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A