Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A9 F1 I1 R1 759 71.0 3673240 91.5% 3361014 104.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,543 Δ1 bp coding (249/276 nt) yjfY ← YhcN family protein, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,5430C.100.0% 207.9 / NA 50coding (249/276 nt)yjfYYhcN family protein, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (28/22);  total (28/22)

AACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NC_000913/4424422‑4424676
                                                                                                                         |                                                                                                                                     
aaCTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGACAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTcccc                                                                                                                     >  1:1489938/1‑139 (MQ=255)
     gTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTc                                                                                                                >  1:767498/1‑139 (MQ=255)
      tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATTGCAAATTTTCGGTGGTTATACCTGGTGGTAGCGATAAATATCCGCGCTGGCGTG‑ATATTTCCGTTAATTCCCGGTTcc                                                                                                               >  1:604969/1‑139 (MQ=255)
      tCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  1:1646021/139‑1 (MQ=255)
               gCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGa                                                                                                      >  2:17739/1‑139 (MQ=255)
                cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGt                                                                                                                  >  1:58621/1‑126 (MQ=255)
                cAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGt                                                                                                                  <  2:58621/126‑1 (MQ=255)
                   ccGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTa                                                                                                  <  1:918027/139‑1 (MQ=255)
                          tCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgta                                                                                           <  2:340186/139‑1 (MQ=255)
                                                   ggCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGtt                                                                  >  1:626503/1‑139 (MQ=255)
                                                       aagcaaaCTATGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTttctt                                                              <  2:626503/139‑1 (MQ=255)
                                                                 tGGCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGc                                                    <  1:17739/139‑1 (MQ=255)
                                                                  ggCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGcc                                                   >  2:1705303/1‑139 (MQ=255)
                                                                   gCAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCt                                                  >  2:1416396/1‑139 (MQ=255)
                                                                    cAATGTTTCGTTGGTTATACGTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTg                                                 >  1:1078805/1‑139 (MQ=255)
                                                                    cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACt                                                                                                                         >  1:973151/1‑67 (MQ=255)
                                                                    cAATGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACt                                                                                                                         <  2:973151/67‑1 (MQ=255)
                                                                      aTGTTTCGTTGGTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGAc                                               <  2:604969/139‑1 (MQ=255)
                                                                      aTGTTTCGTTGGTTATACCTGCTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGAc                                               <  2:1078805/139‑1 (MQ=255)
                                                                                 gTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                >  2:1696433/1‑79 (MQ=255)
                                                                                 gTTATACCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACg                                                                                                <  1:1696433/79‑1 (MQ=255)
                                                                                   tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  1:542306/1‑139 (MQ=255)
                                                                                   tataCCTGGTGTTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTc                                  >  2:529504/1‑139 (MQ=255)
                                                                                           gtgttAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                                            >  2:1049074/5‑89 (MQ=255)
                                                                                           gtgtTAGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTg                                                                            <  1:1049074/89‑1 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCa                                                            <  1:1516284/100‑1 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCa                                                            >  2:1516284/1‑100 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:1674073/1‑139 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:984487/1‑139 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  1:1054862/1‑139 (MQ=255)
                                                                                                aGCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTa                     >  2:922934/1‑139 (MQ=255)
                                                                                                 gCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgt                                                                                            >  1:254605/1‑67 (MQ=255)
                                                                                                 gCGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGgt                                                                                            <  2:254605/67‑1 (MQ=255)
                                                                                                  cGATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtg                   >  2:766740/1‑139 (MQ=255)
                                                                                                   gATAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtga                  <  2:570886/139‑1 (MQ=255)
                                                                                                    aTAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:922934/139‑1 (MQ=255)
                                                                                                    aTAAATATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgat                 <  1:766740/139‑1 (MQ=255)
                                                                                                      aaaTATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGtt                                                                                    <  2:1596718/70‑1 (MQ=255)
                                                                                                      aaaTATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGtt                                                                                    >  1:1596718/1‑70 (MQ=255)
                                                                                                      aaaTATCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTAtgattg               >  1:767164/1‑139 (MQ=255)
                                                                                                         tatCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGata            <  1:529504/139‑1 (MQ=255)
                                                                                                           tCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTcc                                                                                                               <  2:1400753/38‑1 (MQ=37)
                                                                                                           tCCGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTcc                                                                                                               >  1:1400753/1‑38 (MQ=37)
                                                                                                            ccGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg                                                                                 <  2:1757647/67‑1 (MQ=255)
                                                                                                            ccGCGCTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTgcg                                                                                 >  1:1757647/1‑67 (MQ=255)
                                                                                                              gcgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       <  1:1416396/139‑1 (MQ=255)
                                                                                                              gcgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAAt       >  2:968967/1‑139 (MQ=255)
                                                                                                               cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTaa                                                       >  2:1647446/1‑90 (MQ=255)
                                                                                                               cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTaa                                                       <  1:1647446/90‑1 (MQ=255)
                                                                                                               cgcTGGCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATc      >  1:509239/1‑139 (MQ=255)
                                                                                                                   ggCGTG‑ATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGc  >  1:560210/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                     
AACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGTGCTAGCGATAAATATCCGCGCTGGCGTGCATATTGCCGTTACTCCCCGGTTCCCGCATCAGAATTACGTGGTAGTACGTTGCGCCTTGCGCATCTGTTTCTTCATTTAATGCCTGACGTGCTTCGCTTTCAGTGGCGAAATTATGATTGATATAAATCACGC  >  NC_000913/4424422‑4424676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A