Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,616,041	C→T	A93A (GCC→GCT)	yneK →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,616,041	0	C	T	100.0%	91.6 / NA	29	A93A (GCC→GCT)	yneK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/15); total (14/15)

CAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCC  >  NC_000913/1615918‑1616163

cAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGacac                                                                                                             >  2:554264/1‑139 (MQ=255)

            aaaaaaGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATAtt                                                                                                 <  1:554264/139‑1 (MQ=255)

                      tttAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAgg                                                                                       >  2:1217390/1‑139 (MQ=255)

                       ttAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                      <  2:442637/139‑1 (MQ=255)

                        taataaCCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgta                                                                                     <  1:1217390/139‑1 (MQ=255)

                              ccTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                      <  2:134113/132‑1 (MQ=255)

                              ccTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                      >  1:134113/1‑132 (MQ=255)

                                ttGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATAtt                                                                                                 <  2:1414814/119‑1 (MQ=255)

                                ttGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATAtt                                                                                                 >  1:1414814/1‑119 (MQ=255)

                                                     agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                      <  2:736221/109‑1 (MQ=255)

                                                     agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGgt                                                                                      >  1:736221/1‑109 (MQ=255)

                                                     agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCtatttat                                                             <  1:1361479/134‑1 (MQ=255)

                                                     agagGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCtatttat                                                             >  2:1361479/1‑134 (MQ=255)

                                                       agGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATa                                                      >  2:773416/1‑139 (MQ=255)

                                                            ttcttTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGacac                                                                                                             <  1:1330908/79‑1 (MQ=255)

                                                            ttcttTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGacac                                                                                                             >  2:1330908/1‑79 (MQ=255)

                                                                      ggTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTa                                                                         >  1:1312084/1‑105 (MQ=255)

                                                                      ggTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTa                                                                         <  2:1312084/105‑1 (MQ=255)

                                                                                               aGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGc                                              >  2:126956/1‑107 (MQ=255)

                                                                                               aGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGc                                              <  1:126956/107‑1 (MQ=255)

                                                                                               aGGTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTcc              >  1:812801/1‑139 (MQ=255)

                                                                                                ggTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTccc             <  1:773416/139‑1 (MQ=255)

                                                                                                 gTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGa                                                                                                        <  1:1234912/47‑1 (MQ=255)

                                                                                                 gTTCAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGa                                                                                                        >  2:1234912/1‑47 (MQ=255)

                                                                                                    cAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTTTTTATTTAGATAATTTTCGCCatgatg                                       <  2:1436219/109‑1 (MQ=255)

                                                                                                    cAGTTTAAACTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTTTTTATTTAGATAATTTTCGCCatgatg                                       >  1:1436219/1‑109 (MQ=255)

                                                                                                           aaCTCACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGcc  <  2:812801/139‑1 (MQ=255)

                                                                                                               cACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCta                                                                  <  2:130402/71‑1 (MQ=255)

                                                                                                               cACTTATCCCGCTAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCta                                                                  >  1:130402/1‑71 (MQ=255)

CAACTCAGCCACAAAAAAGATATTTAATAACCTTGACAACTTATCATATATTCAGAGGACTTCTTTTCGGGGTAACGACACGCTATACAATGAAAAGGTTCAGTTTAAACTCACTTATCCCGCCAGGAATGGAAGACACAAAGAAAATATTGAATTTCAGGTAGTAATAAATTTAAGCCCTATTTATTTAGATAATTTTCGCCATGATGGAGAAATTAATATTTTTTGCGCTCCCAACCCAAAGCC  >  NC_000913/1615918‑1616163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A