Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I1 R1	760	58.2	3022526	90.8%	2744453	102.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,769,478	(G)_7→10	coding (405/1113 nt)	ydiK →	UPF0118 family inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1769471	0 (0.000)	42 (0.750) +GGG	32/182	0.5	100%	coding (398/1113 nt)	ydiK	UPF0118 family inner membrane protein
*	?	NC_000913	1769472 =	0 (0.000)	42 (0.750) +GGG	32/182	0.5	100%	coding (399/1113 nt)	ydiK	UPF0118 family inner membrane protein

CGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1769340‑1769471

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC  >  NC_000913/1769472‑1769599

                                                                                                                                    ||||

CGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGG                                                                                                                              >  1:758894/1‑139

            CGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATC                                                                                                                  >  2:1143408/1‑139

            CGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGG                                                                                                                           <  2:550640/130‑1

            CGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGG                                                                                                                           >  1:550640/1‑130

                 CGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGC                                                                                                             >  1:446128/1‑139

                        TAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTC                                                                                                      <  2:446128/139‑1

                             CCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGTCGAAAGTCCGCCC                                                                                                 >  1:1092621/1‑139

                                 TTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTC                                                                                                      <  2:756748/130‑1

                                 TTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTC                                                                                                      >  1:756748/1‑130

                                  TCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTAT                                                                                             >  2:1346236/1‑139

                                  TCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATA                                                                                            >  1:895603/1‑139

                                        GGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCA                                                                                      <  1:1143408/139‑1

                                          TGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGGGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCAC                                                                                     <  1:1346236/139‑1

                                                   GTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACAGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACC                                                                           >  1:557555/1‑139

                                                            TCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCAAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGAC                                                                                                                         >  2:400168/1‑84

                                                            TCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCAAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGAC                                                                                                                         <  1:400168/84‑1

                                                               GGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCT                                                                                                >  2:503692/1‑106

                                                               GGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCT                                                                                                <  1:503692/106‑1

                                                                GCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGAT                                                                                                                   >  2:684730/1‑86

                                                                GCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGAT                                                                                                                   <  1:684730/86‑1

                                                                 CGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCA                                                             >  1:1418364/1‑139

                                                                                TTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGG                                              >  1:432322/1‑139

                                                                                   CGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGC                                                          >  1:677430/1‑124

                                                                                   CGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGC                                                          <  2:677430/124‑1

                                                                                           GGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGT                                  >  2:878201/1‑139

                                                                                            GCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCG                                                                                                            <  2:32533/65‑1

                                                                                            GCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCG                                                                                                            >  1:32533/1‑65

                                                                                                      GTATGCAGGCTGGCACAACTTGCTGGATAT‑‑GGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGC                       <  1:878201/139‑1

                                                                                                          GCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGA                    <  2:959288/139‑1

                                                                                                           CAGGCTGGCACAACTTGCTGGATATGGGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGA                    <  2:758894/139‑1

                                                                                                                 GGCACAACTTGCTGGGTAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCG                                           <  1:1016567/109‑1

                                                                                                                 GGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCT             >  1:926367/1‑139

                                                                                                                 GGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCG                                           >  2:1016567/1‑109

                                                                                                                 GGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTC                                                                                                      >  2:1050099/1‑50

                                                                                                                 GGCACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTC                                                                                                      <  1:1050099/50‑1

                                                                                                                   CACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCT           >  2:421206/1‑139

                                                                                                                   CACAACTTGCTGGATAT‑‑GGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTT          >  1:380329/1‑139

                                                                                                                    ACAACTTGCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTT          <  2:926367/139‑1

                                                                                                                          TGCTGGATAT‑GGGGGGGGGGACAGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGC    <  2:557555/139‑1

                                                                                                                           GCTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCC   <  2:432322/139‑1

                                                                                                                            CTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC  <  2:1092621/139‑1

                                                                                                                            CTGGATAT‑GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC  <  1:421206/139‑1

                                                                                                                                     GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCG                                           >  2:560793/1‑90

                                                                                                                                     GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCG                                           <  1:560793/90‑1

                                                                                                                                     GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGG                                                        >  2:1454142/1‑77

                                                                                                                                     GGGGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGG                                                        <  1:1454142/77‑1

                                                                                                                                       GGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCA                                                                                   <  2:1118529/48‑1

                                                                                                                                       GGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCA                                                                                   >  1:1118529/1‑48

                                                                                                                                    ||||

CGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1769340‑1769471

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCC  >  NC_000913/1769472‑1769599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A