Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 760 58.2 3022526 90.8% 2744453 102.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,158,586 A→G S33G (AGC→GGC)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,158,5860AG100.0% 261.5 / NA 71S33G (AGC→GGC) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/31);  total (40/31)

CCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTC  >  NC_000913/4158451‑4158720
                                                                                                                                       |                                                                                                                                      
ccTATCGCCGTGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTAGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTTGCCAGCCGACGCTTGGCg                                                                                                                                     >  1:1013522/1‑139 (MQ=255)
    tCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACACTTGGCGGGCa                                                                                                                                 <  1:755391/139‑1 (MQ=255)
          tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCg                                                                                                                           >  1:401201/1‑139 (MQ=255)
          tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCg                                                                                                                           >  1:1428189/1‑139 (MQ=255)
          tGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCg                                                                                                                           >  1:1269027/1‑139 (MQ=255)
           gAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGt                                                                                                                          <  2:1428189/139‑1 (MQ=255)
             aCTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCAGACGCTTGGCGGGCAAATTCGTaa                                                                                                                        <  2:401201/139‑1 (MQ=255)
                      gCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTAGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAGGCTGGAAGa                                                                                                               <  2:1013522/139‑1 (MQ=255)
                       cGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGAt                                                                                                              >  1:1286580/1‑139 (MQ=255)
                           ggaggaTGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                                          <  2:295258/139‑1 (MQ=255)
                                    atAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                                                 >  1:1148444/1‑139 (MQ=255)
                                         gAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTg                                                                                            >  2:494400/1‑139 (MQ=255)
                                             atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGaa                                                                                                                 <  2:2491/114‑1 (MQ=255)
                                             atTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGaa                                                                                                                 >  1:2491/1‑114 (MQ=255)
                                              ttCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGa                                                                                       >  1:1149287/1‑139 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACGCCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                                                                      >  1:327134/1‑139 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                                          >  1:128202/1‑103 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGc                                                                                                          <  2:128202/103‑1 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  1:746304/1‑128 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  2:746304/128‑1 (MQ=255)
                                                               cTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAg                                                                      >  2:925968/1‑139 (MQ=255)
                                                                 ggtggCATTGGCTGAACAGCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTATAgtg                                                                    >  1:993183/1‑139 (MQ=255)
                                                                 ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 <  1:853152/126‑1 (MQ=255)
                                                                 ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGAc                                                                                 >  2:853152/1‑126 (MQ=255)
                                                                 ggtggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgtg                                                                    >  1:1357391/1‑139 (MQ=255)
                                                                   tggCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGtgtt                                                                  >  1:895919/1‑139 (MQ=255)
                                                                      cATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTgttgtt                                                               >  1:144855/1‑139 (MQ=255)
                                                                        ttGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCa                                                             >  1:1362209/1‑139 (MQ=255)
                                                                         tGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                                            <  1:925968/139‑1 (MQ=255)
                                                                           gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAt                                                                                                <  2:560635/101‑1 (MQ=255)
                                                                           gCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGAt                                                                                                >  1:560635/1‑101 (MQ=255)
                                                                           gCTGAACACCGCCACTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTggg                                                                                                      >  2:1384078/1‑95 (MQ=255)
                                                                           gCTGAACACCGCCACTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTggg                                                                                                      <  1:1384078/95‑1 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCggg                                                                                                                                   <  1:541864/57‑1 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCggg                                                                                                                                   >  2:541864/1‑57 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGAt                                                                                                              >  1:1505998/1‑78 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGAt                                                                                                              <  2:1505998/78‑1 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCt                                                                                                         >  1:1042711/1‑83 (MQ=255)
                                                                                    cGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCt                                                                                                         <  2:1042711/83‑1 (MQ=255)
                                                                                     gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                         >  1:993036/1‑114 (MQ=255)
                                                                                     gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTa                                                                         <  2:993036/114‑1 (MQ=255)
                                                                                     gCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAt                                                >  2:1508116/1‑139 (MQ=255)
                                                                                      ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                               <  1:308645/139‑1 (MQ=255)
                                                                                      ccATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtg                                               >  2:308645/1‑139 (MQ=255)
                                                                                       cATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                                              <  2:1148444/139‑1 (MQ=255)
                                                                                       cATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgc                                              <  2:1362209/139‑1 (MQ=255)
                                                                                        aTTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAAtgct                                             <  2:1286580/139‑1 (MQ=255)
                                                                                           ttCGGCGTGCGGCAGATTCCTGCTACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATgctgct                                          <  2:993183/139‑1 (MQ=255)
                                                                                              ggCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCtggt                                       >  2:382753/1‑139 (MQ=255)
                                                                                                 gTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGa                                    >  2:622470/1‑139 (MQ=255)
                                                                                                       cAGATTCCTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGgc                              <  2:1269027/139‑1 (MQ=255)
                                                                                                              cTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                                            >  2:305938/1‑102 (MQ=255)
                                                                                                              cTGCCACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCAc                                                            <  1:305938/102‑1 (MQ=255)
                                                                                                                 ccACGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGtg                    >  2:469296/1‑139 (MQ=255)
                                                                                                                    cGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                     <  2:1387192/87‑1 (MQ=255)
                                                                                                                    cGTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAgt                                                                     >  1:1387192/1‑87 (MQ=255)
                                                                                                                     gTTAGCCAGCCGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGtgctgc                >  2:773044/1‑139 (MQ=255)
                                                                                                                              ccGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGa       >  2:1112574/1‑139 (MQ=255)
                                                                                                                              ccGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGa       <  1:469296/139‑1 (MQ=255)
                                                                                                                               cGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:1112574/139‑1 (MQ=255)
                                                                                                                               cGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:773044/139‑1 (MQ=255)
                                                                                                                               cGACGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGaa      <  1:1508116/139‑1 (MQ=255)
                                                                                                                                 aCGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                                                 >  1:1042695/1‑46 (MQ=255)
                                                                                                                                 aCGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGa                                                                                                 <  2:1042695/46‑1 (MQ=255)
                                                                                                                                 aCGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAg    >  2:672248/1‑139 (MQ=255)
                                                                                                                                  cGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                              <  2:502177/64‑1 (MQ=255)
                                                                                                                                  cGCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAg                                                                              >  1:502177/1‑64 (MQ=255)
                                                                                                                                   gCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      >  1:982335/1‑55 (MQ=255)
                                                                                                                                   gCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAg                                                                                      <  2:982335/55‑1 (MQ=255)
                                                                                                                                   gCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTc  >  1:1284610/1‑139 (MQ=255)
                                                                                                                                   gCTTGGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTAATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTc  >  1:985385/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CCTATCGCCATGAACTATCGTGGCGATGGAGGATGGATAATGAATATTCGTGATCTTGAGTACCTGGTGGCATTGGCTGAACACCGCCATTTTCGGCGTGCGGCAGATTCCTGCCACGTTAGCCAGCCGACGCTTAGCGGGCAAATTCGTAAGCTGGAAGATGAGCTGGGCGTGATGTTGCTGGAGCGGACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTC  >  NC_000913/4158451‑4158720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A