Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
758 |
59.3 |
2864096 |
93.9% |
2689386 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
860,638 |
A→G |
Y657H (TAT→CAT) |
ybiW ← |
putative pyruvate formate lyase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 860,638 | 0 | A | G | 100.0%
| 119.4
/ NA
| 33 | Y657H (TAT→CAT) | ybiW | putative pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/15); total (18/15) |
TCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGC > NC_000913/860514‑860764
|
tCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCg < 1:132060/139‑1 (MQ=255)
ggCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg < 2:675579/139‑1 (MQ=255)
tGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGa < 1:870844/139‑1 (MQ=255)
cgcCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGAtt > 2:244962/1‑139 (MQ=255)
tttACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 2:897027/139‑1 (MQ=255)
tCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCa < 2:388805/87‑1 (MQ=255)
tCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCa > 1:388805/1‑87 (MQ=255)
tCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg > 2:804112/1‑98 (MQ=255)
tCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgcg < 1:804112/98‑1 (MQ=255)
ccATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa < 2:1406080/137‑1 (MQ=255)
ccATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa > 1:1406080/1‑137 (MQ=255)
aGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcagc < 1:244962/139‑1 (MQ=255)
gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa > 2:746824/1‑95 (MQ=255)
gTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa < 1:746824/95‑1 (MQ=255)
cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt < 2:145874/104‑1 (MQ=255)
cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtcgt > 1:145874/1‑104 (MQ=255)
cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa > 1:836206/1‑139 (MQ=255)
cgcgcCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa > 2:981683/1‑139 (MQ=255)
cAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGAc > 1:387026/1‑139 (MQ=255)
gCGGAGATTTATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgc < 1:1163022/58‑1 (MQ=255)
gCGGAGATTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAgc > 2:1163022/1‑58 (MQ=255)
aTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc > 1:1086604/1‑116 (MQ=255)
aTTGATGACGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc < 2:1086604/116‑1 (MQ=255)
tgaCGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTc > 1:1049788/1‑139 (MQ=255)
cGTACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgc > 1:1405006/1‑139 (MQ=255)
tACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcgc > 1:844682/1‑139 (MQ=255)
tACCCGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGgcgc > 2:42971/1‑139 (MQ=255)
cccGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg < 2:653979/43‑1 (MQ=255)
cccGCGTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg > 1:653979/1‑43 (MQ=255)
cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg < 1:75575/41‑1 (MQ=255)
cgcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGcgcg > 2:75575/1‑41 (MQ=255)
gcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa > 2:341275/1‑50 (MQ=255)
gcgTAATGGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTa < 1:341275/50‑1 (MQ=255)
|
TCAGTACCGGAGGCCGGGCTTGCGCCTTCTGCCAGCGGGGTGTGGGCTTTACGCCCGTCCGGTGTTGCCATAGTCTGCGCGCCAAACGGTACGTTAGCGGAGATTGATGACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTCGGCGC > NC_000913/860514‑860764
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
