Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	877,698	A→G	V113A (GTT→GCT)	rimO ←	ribosomal protein S12 methylthiotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	877,698	0	A	G	100.0%	227.2 / NA	63	V113A (GTT→GCT)	rimO	ribosomal protein S12 methylthiotransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/30); total (33/30)

TGCAGAAGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGC  >  NC_000913/877564‑877828

tGCAGAAGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTg                                                                                                                                >  1:597938/1‑139 (MQ=255)

     aaGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGTCACCAGACTCAGGAATGGGTTGCGTTTCGGTTTTGGCACGTAGTGATGAGCg                                                                                                                                  <  2:826494/132‑1 (MQ=255)

     aaGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCg                                                                                                                                  >  1:826494/1‑132 (MQ=255)

      aGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAg                                                                                                                          <  2:698282/139‑1 (MQ=255)

           cAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCt                                                                                                                     >  1:817285/1‑139 (MQ=255)

           cAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCt                                                                                                                     >  2:1241385/1‑139 (MQ=255)

            aGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTg                                                                                                                    >  1:101834/1‑139 (MQ=255)

               ggTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAAc                                                                                                                       <  2:779997/133‑1 (MQ=255)

               ggTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAAc                                                                                                                       >  1:779997/1‑133 (MQ=255)

                  gATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATa                                                                                                              <  2:101834/139‑1 (MQ=255)

                   aTTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAg                                                                                                             >  2:1140706/1‑139 (MQ=255)

                   aTTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAg                                                                                                             >  1:898624/1‑139 (MQ=255)

                       cAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTAt                                                                                                         <  1:1140706/139‑1 (MQ=255)

                        aGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATg                                                                                                        >  1:162001/1‑139 (MQ=255)

                             tCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGcc                                                                                                   >  2:550896/1‑139 (MQ=255)

                             tCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGcc                                                                                                   >  2:31988/1‑139 (MQ=255)

                             tCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGcc                                                                                                   >  2:574273/1‑139 (MQ=255)

                              cAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  >  1:1333540/1‑123 (MQ=255)

                              cAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  <  2:1333540/123‑1 (MQ=255)

                              cAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGccc                                                                                                  >  2:200174/1‑139 (MQ=255)

                                     tttCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGAtt                                                                                           <  2:898624/139‑1 (MQ=255)

                                          gATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAg                                                                                      <  1:483004/139‑1 (MQ=255)

                                                   aaTGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCgg                                                                             <  1:1318774/139‑1 (MQ=255)

                                                         gcggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  >  2:143060/1‑96 (MQ=255)

                                                         gcggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGAc                                                                       >  2:615431/1‑139 (MQ=255)

                                                         gcggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGAc                                                                       >  1:487843/1‑139 (MQ=255)

                                                         gcggcgTCAGTTTCACACCTTGTTCTGGCAACAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  <  1:143060/96‑1 (MQ=255)

                                                          cggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAAc                                                                                                                       >  2:1217135/1‑90 (MQ=255)

                                                          cggcgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAAc                                                                                                                       <  1:1217135/90‑1 (MQ=255)

                                                             cgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCg                                                                                                                                  <  1:1225535/76‑1 (MQ=255)

                                                             cgTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGATCCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTcg                                                                   <  2:817285/139‑1 (MQ=255)

                                                                   tttCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGgatc                                                             <  2:597938/139‑1 (MQ=255)

                                                                       acacCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGgatctgat                                                         <  1:1241385/139‑1 (MQ=255)

                                                                             tGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTg                                                                                              <  2:1065918/96‑1 (MQ=255)

                                                                             tGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTg                                                                                              >  1:1065918/1‑96 (MQ=255)

                                                                             tGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATcttctt                                                   >  2:1042831/1‑139 (MQ=255)

                                                                               ttCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCtttt                                                 >  2:570/1‑139 (MQ=255)

                                                                               ttCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCtttt                                                 >  2:592559/1‑139 (MQ=255)

                                                                                 cTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGc                                               <  1:570/139‑1 (MQ=255)

                                                                                        cAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGa                                        <  2:487843/139‑1 (MQ=255)

                                                                                           gCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACaa                                     <  1:241799/139‑1 (MQ=255)

                                                                                                ggAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGt                                <  1:31988/139‑1 (MQ=255)

                                                                                                     gggTTGTGTTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACaa                           >  1:1336681/1‑139 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  <  2:1392331/46‑1 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCt                                                                                                                  >  1:1392331/1‑46 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAg                                                                                      <  1:890905/74‑1 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAg                                                                                      >  2:890905/1‑74 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGa                                        <  2:1337102/120‑1 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGa                                        >  1:1337102/1‑120 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACaa                                     >  2:754033/1‑123 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACaa                                     <  1:754033/123‑1 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCt                     >  2:711856/1‑139 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCt                     >  1:580205/1‑139 (MQ=255)

                                                                                                           tgtTTCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCt                     >  1:682198/1‑139 (MQ=255)

                                                                                                              ttCGGTTTTGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGc                  <  2:162001/139‑1 (MQ=255)

                                                                                                                cGGTTTTGGCACGTAGTGATAAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCg                >  2:557810/1‑139 (MQ=255)

                                                                                                                 gtttttGGCACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGt               >  2:859059/3‑139 (MQ=255)

                                                                                                                         cACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTttcattc       <  1:550896/139‑1 (MQ=255)

                                                                                                                         cACGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTttcattc       <  1:1042831/139‑1 (MQ=255)

                                                                                                                           cGTAGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCaa     <  2:580205/139‑1 (MQ=255)

                                                                                                                              aGTGATGAGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGc  <  1:557810/139‑1 (MQ=255)

                                                                                                                                  atgaGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCtttt                                                 >  2:213504/1‑88 (MQ=255)

                                                                                                                                  atgaGCGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCtttt                                                 <  1:213504/88‑1 (MQ=255)

TGCAGAAGGTGCAGCGGTGATTACAGCCTTCAGAAATTTTCAGATAGGCATAATGACGCGGCGTCAGTTTCACACCTTGTTCTGGCACCAGGCTCAGGAATGGGTTGTGTTTCGGTTTTGGCACGTAGTGATGAACGTGCTCCAGAACCTGCTCATAGCTATGAGGCCCGGTGATTTCCAGCACTTTCGGGTGGACTTCGCGGATCTGATCTTCTTTTGCCCCCAGACAACCGGTCACAATAACCTTGCCGTTTTCATTCAACGC  >  NC_000913/877564‑877828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A