Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F1 I2 R1 758 59.3 2864096 93.9% 2689386 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 983,623 G→A *183* (TAG→TAA)  ycbK → M15A protease‑related family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913983,6230GA100.0% 182.5 / NA 57*183* (TAG→TAA) ycbKM15A protease‑related family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/25);  total (32/25)

ATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGG  >  NC_000913/983501‑983744
                                                                                                                          |                                                                                                                         
atTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGaaa                                                                                                           <  2:1212036/139‑1 (MQ=255)
 ttGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                               >  1:495213/1‑134 (MQ=255)
 ttGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                               <  2:495213/134‑1 (MQ=255)
         aTCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAg                                                                                                  >  1:861058/1‑139 (MQ=255)
                                 gCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGt                                                                          >  2:631954/1‑139 (MQ=255)
                                   cgcgTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     <  1:446094/110‑1 (MQ=255)
                                   cgcgTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     >  2:446094/1‑110 (MQ=255)
                                                   gcAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                        <  2:861058/139‑1 (MQ=255)
                                                    cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTAt                                                                                        <  1:353690/106‑1 (MQ=255)
                                                    cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTAt                                                                                        >  2:353690/1‑106 (MQ=255)
                                                    cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATtct                                                               >  2:819969/1‑131 (MQ=255)
                                                    cAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATtct                                                               <  1:819969/131‑1 (MQ=255)
                                                          gtgtAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGTTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  <  2:406226/122‑1 (MQ=255)
                                                          gtgtAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGTTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  >  1:406226/1‑122 (MQ=255)
                                                               ggATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAAt                                            <  1:631954/139‑1 (MQ=255)
                                                                 atatTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                        <  2:44893/125‑1 (MQ=255)
                                                                 atatTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGaa                                                        >  1:44893/1‑125 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                               >  2:1051495/1‑66 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAAc                                                                                                               <  1:1051495/66‑1 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTcc                                                             >  2:591077/1‑116 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTcc                                                             <  1:591077/116‑1 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGgtg                                      >  1:52649/1‑139 (MQ=255)
                                                                     tatCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGGAATAATTGCTTAACGAAAc                                                                                                          <  1:955672/71‑1 (MQ=255)
                                                                     tatCCACGTAGTAACATTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAAc                                                                                                          >  2:955672/1‑71 (MQ=255)
                                                                       tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  >  2:221261/1‑109 (MQ=255)
                                                                       tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  <  1:221261/109‑1 (MQ=255)
                                                                       tCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGtgtg                                    >  1:213124/1‑139 (MQ=255)
                                                                            gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGgtaata                                                                                                                         >  2:785431/1‑49 (MQ=255)
                                                                            gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                      <  1:1150535/68‑1 (MQ=255)
                                                                            gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAggg                                                                                                      >  2:1150535/1‑68 (MQ=255)
                                                                            gtagtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACaa                               >  1:1022313/1‑139 (MQ=255)
                                                                              agtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAAt                                            <  2:853072/124‑1 (MQ=255)
                                                                              agtaACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCCGTCACCGCATTCTCCCAGAACTGTTCATTAAt                                            >  1:853072/1‑124 (MQ=255)
                                                                                taACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCa                                                 >  1:1211077/1‑117 (MQ=255)
                                                                                taACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCa                                                 <  2:1211077/117‑1 (MQ=255)
                                                                                        tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  >  1:920796/1‑92 (MQ=255)
                                                                                        tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  <  2:920796/92‑1 (MQ=255)
                                                                                        tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGcc                   >  1:510914/1‑139 (MQ=255)
                                                                                        tgCATATTGATACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGcc                   >  1:721267/1‑139 (MQ=255)
                                                                                             atTGATACCGGGCCAGCACGGCACTGGTAATACTTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACt              >  2:851571/1‑139 (MQ=255)
                                                                                                 aTACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  <  2:1114747/83‑1 (MQ=255)
                                                                                                 aTACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  >  1:1114747/1‑83 (MQ=255)
                                                                                                 aTACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCCTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTc          <  2:1022313/139‑1 (MQ=255)
                                                                                                  tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     <  1:1006288/47‑1 (MQ=255)
                                                                                                  tACCGGGCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     >  2:1006288/1‑47 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     <  1:1158640/42‑1 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     >  2:1158640/1‑42 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGt                                                                                                 <  2:77939/46‑1 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGt                                                                                                 >  1:77939/1‑46 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  <  2:495402/77‑1 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCAt                                                                  >  1:495402/1‑77 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt    >  1:1391124/1‑139 (MQ=255)
                                                                                                       ggCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCt    >  2:359968/1‑139 (MQ=255)
                                                                                                       agCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                         >  1:1288573/2‑54 (MQ=255)
                                                                                                       agCCAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTa                                                                                         <  2:1288573/53‑1 (MQ=255)
                                                                                                         ccAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     <  2:543506/40‑1 (MQ=255)
                                                                                                         ccAGCACGGCACTGGTAATAATTGCTTAACGAAACAgggg                                                                                                     >  1:543506/1‑40 (MQ=255)
                                                                                                         ccAGCACGGCACTGGTAATAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTgg  >  1:1288959/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                         
ATTGAAGGTATCGCGTTAAGCAATATTCGCAAAGCCGCGTTATCTATGCGCGCAGGTGGTGTAGGATATTATCCACGTAGTAACTTTGTGCATATTGATACCGGGCCAGCACGGCACTGGTAGTAATTGCTTAACGAAACAGGGGCAGTATGAACTATCGTATTATTCCGGTCACCGCATTCTCCCAGAACTGTTCATTAATCTGGTGTGAACAAACCCGTCTGGCCGCACTGGTCGATCCTGG  >  NC_000913/983501‑983744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A