Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,054,899	A→G	V427A (GTG→GCG)	torS ←	hybrid sensory histidine kinase in two‑component regulatory system with TorR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,054,899	0	A	G	100.0%	172.5 / NA	48	V427A (GTG→GCG)	torS	hybrid sensory histidine kinase in two‑component regulatory system with TorR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

TGCCAGCAGTTGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCACCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTA  >  NC_000913/1054769‑1055025

tGCCAGCAGTTGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTcc                                                                                                                        >  1:1199911/1‑139 (MQ=255)

  ccAGCAGTTGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTg                                                                                                                      <  2:1199911/139‑1 (MQ=255)

               gttgcctggactACCGTTCATCTGTGTGCGGATCTCATGGCTCATCGCCGCCGGAAACGCCGATTTCACCTGGCTCGCTTTTTCTGCTGTCGCCCTTGTCTGTCGGTGTTCTACCGCCAGTTCCTGCAATTCAGCGGTa                                                                                                         <  2:1231293/128‑1 (MQ=255)

                 tGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTAcg                                                                                                       >  2:809478/1‑139 (MQ=255)

                           aCCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGAcc                                                                                             >  1:1321572/1‑139 (MQ=255)

                           aCCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTAAGCGCTTTGAcc                                                                                             >  1:1231293/1‑139 (MQ=255)

                                  aGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGcgccg                                                                                      >  2:163206/1‑139 (MQ=255)

                                  aGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGcgccg                                                                                      >  2:637784/1‑139 (MQ=255)

                                  aGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGcgccg                                                                                      >  1:224482/1‑139 (MQ=255)

                                              aTCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCa                                                                                   >  2:595966/1‑130 (MQ=255)

                                              aTCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCa                                                                                   <  1:595966/130‑1 (MQ=255)

                                              aTCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTcgc                                                                          <  2:594906/139‑1 (MQ=255)

                                                ctcATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCgg                                                                        <  1:809478/139‑1 (MQ=255)

                                                 tcATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTg                                                                                                                      >  2:763521/1‑92 (MQ=255)

                                                 tcATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTg                                                                                                                      <  1:763521/92‑1 (MQ=255)

                                                   aTGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTgg                                                                     <  2:736326/139‑1 (MQ=255)

                                                         cATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTc                                                               <  1:1430833/139‑1 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCt                                                                                            <  2:1218929/109‑1 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCt                                                                                            >  1:1218929/1‑109 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCa                                                                                   <  2:716617/118‑1 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCa                                                                                   >  1:716617/1‑118 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCgg                                                                        >  1:927080/1‑129 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCgg                                                                        <  2:927080/129‑1 (MQ=255)

                                                          aTCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCa                                                              >  1:528472/1‑139 (MQ=255)

                                                                 ccAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGAtt                                                                >  2:1160011/1‑130 (MQ=255)

                                                                 ccAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGAtt                                                                <  1:1160011/130‑1 (MQ=255)

                                                                     aaaCGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACa                                                   <  2:224482/139‑1 (MQ=255)

                                                                             aTTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCg                                           <  1:637784/139‑1 (MQ=255)

                                                                                     tggctggcTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGAtt                                                                >  2:513887/1‑110 (MQ=255)

                                                                                     tggctggcTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGAtt                                                                <  1:513887/110‑1 (MQ=255)

                                                                                       gctggctTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAg                                                                                                              <  1:154263/62‑1 (MQ=255)

                                                                                       gctggctTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAg                                                                                                              >  2:154263/1‑62 (MQ=255)

                                                                                       gctggctTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcatcc                                 >  1:1088498/1‑139 (MQ=255)

                                                                                         tggctTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGcatccat                               <  2:1321572/139‑1 (MQ=255)

                                                                                         tggctTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAAAGcatccat                               >  2:1000803/1‑139 (MQ=255)

                                                                                                 tCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAgccgcc                       <  1:1000803/139‑1 (MQ=255)

                                                                                                    gCTTCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGa                    <  1:163206/139‑1 (MQ=255)

                                                                                                       tCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATgg                 <  2:1213793/139‑1 (MQ=255)

                                                                                                       tCCGCCCGTGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATgg                 >  1:1213793/1‑139 (MQ=255)

                                                                                                               tGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGc         <  2:240989/139‑1 (MQ=255)

                                                                                                               tGCCTGTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGc         >  1:240989/1‑139 (MQ=255)

                                                                                                                    gTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACg                                      <  2:1320662/105‑1 (MQ=255)

                                                                                                                    gTCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACg                                      >  1:1320662/1‑105 (MQ=255)

                                                                                                                     tCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGAt                   <  2:1277835/123‑1 (MQ=255)

                                                                                                                     tCGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGAt                   >  1:1277835/1‑123 (MQ=255)

                                                                                                                      cGGTGTTCTATCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTa  >  2:430456/1‑139 (MQ=255)

                                                                                                                               aTCGCCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTc                                                               >  1:923734/1‑69 (MQ=255)

                                                                                                                               aTCGCCACTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTc                                                               <  2:923734/69‑1 (MQ=255)

TGCCAGCAGTTGAGCAGTGCCGAGAATACCGTACAGCGGTGTGCGGATCTCATGGCTCATCGCCGCCAGAAACGCCGATTTTGCCTGGCTGGCTTTTTCTGCTTCCGCCCGTGCCTGTCGGTGTTCTATCACCAGTTCCTGCAATTCAGCTGTACGCGCTTTGACCTGCGCCGCCAGCTGTTCGCGGTGGCGATTCAGTGCATGAACATTGCTGCGAAACGCATCCATCAGCCGCCCGATGGTATCCAGCTCCCGTA  >  NC_000913/1054769‑1055025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A