Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,819,569	A→G	G79G (GGT→GGC)	chbA ←	N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,819,569	0	A	G	100.0%	212.3 / NA	59	G79G (GGT→GGC)	chbA	N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/26); total (33/26)

GCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGC  >  NC_000913/1819438‑1819697

gCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGc                                                                                                                           <  1:1073384/139‑1 (MQ=255)

   gCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCg                                                                                                                        >  1:363969/1‑139 (MQ=255)

                   aTGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAg                                                                                                        >  2:594370/1‑139 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCg                                                                                                   <  2:777142/137‑1 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCg                                                                                                   >  1:777142/1‑137 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 >  1:1267453/1‑139 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 >  2:717020/1‑139 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 >  1:616065/1‑139 (MQ=255)

                          cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 >  2:868290/1‑139 (MQ=255)

                                ttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTAcc                                                                                           >  2:1326845/1‑139 (MQ=255)

                                    tGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                       <  1:717020/139‑1 (MQ=255)

                                       aGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTa                                                                                             <  1:878320/130‑1 (MQ=255)

                                       aGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTa                                                                                             >  2:878320/1‑130 (MQ=255)

                                            aattaattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCt                                                                                  >  2:228964/1‑136 (MQ=255)

                                            aattaattCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCt                                                                                  <  1:228964/136‑1 (MQ=255)

                                                   tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                        >  1:538689/1‑139 (MQ=255)

                                                   tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                        <  2:1267453/139‑1 (MQ=255)

                                                   tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                        <  1:249872/139‑1 (MQ=255)

                                                        aaTCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATc                                                                   <  1:717979/139‑1 (MQ=255)

                                                        aaTCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATc                                                                   <  1:206330/139‑1 (MQ=255)

                                                              ttCACGTGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGAc                                                             >  1:493841/1‑139 (MQ=255)

                                                                  cgcgCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGAt                                                         <  1:1326845/139‑1 (MQ=255)

                                                                            tGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGc                                               >  1:427027/1‑139 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCa                                                                                                                      <  2:972589/61‑1 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCa                                                                                                                      >  1:972589/1‑61 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTAc                                                                                            <  2:97412/87‑1 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTAc                                                                                            >  1:97412/1‑87 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                        >  1:770647/1‑139 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                        >  1:1289955/1‑139 (MQ=255)

                                                                                   cATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTtgct                                        >  1:1262390/1‑139 (MQ=255)

                                                                                             tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGa                                                              >  1:446648/1‑107 (MQ=255)

                                                                                             tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGa                                                              <  2:446648/107‑1 (MQ=255)

                                                                                             tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                              >  2:875586/1‑139 (MQ=255)

                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCTCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATc                             >  1:699212/1‑139 (MQ=255)

                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGt                                                                                                       <  1:1090764/65‑1 (MQ=255)

                                                                                              ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGt                                                                                                       >  2:1090764/1‑65 (MQ=255)

                                                                                                      tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                     >  2:432472/1‑139 (MQ=255)

                                                                                                      tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                     <  1:432472/139‑1 (MQ=255)

                                                                                                      tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                     <  2:493841/139‑1 (MQ=255)

                                                                                                      tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCt                     >  2:611928/1‑139 (MQ=255)

                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGt                                                                >  2:1096481/1‑95 (MQ=255)

                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGt                                                                <  1:1096481/95‑1 (MQ=255)

                                                                                                       ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                    >  1:1193466/1‑139 (MQ=255)

                                                                                                         aCCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCg                  >  2:667004/1‑139 (MQ=255)

                                                                                                          ccAGCACCAGACTCACTTTCATCTTGCCTTCGCTCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGc                 <  2:699212/139‑1 (MQ=255)

                                                                                                          ccAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGc                 <  2:770647/139‑1 (MQ=255)

                                                                                                              cACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                     >  2:980676/1‑35 (MQ=255)

                                                                                                              cACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                     <  1:980676/35‑1 (MQ=255)

                                                                                                              cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGt             >  2:44167/1‑139 (MQ=255)

                                                                                                              cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGt             >  2:251883/1‑139 (MQ=255)

                                                                                                               aCCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGc                                                                       >  2:506438/1‑80 (MQ=255)

                                                                                                               aCCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGc                                                                       <  1:506438/80‑1 (MQ=255)

                                                                                                                   gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAg        >  1:867671/1‑139 (MQ=255)

                                                                                                                     cTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTg      <  1:875586/139‑1 (MQ=255)

                                                                                                                     cTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTg      <  1:251883/139‑1 (MQ=255)

                                                                                                                        aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 <  1:282896/45‑1 (MQ=255)

                                                                                                                        aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                 >  2:282896/1‑45 (MQ=255)

                                                                                                                         cTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGc  <  2:1289955/139‑1 (MQ=255)

                                                                                                                         cTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTCCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGc  <  2:616065/139‑1 (MQ=255)

GCTGCATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGC  >  NC_000913/1819438‑1819697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A