Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,159,059	(A)_5→4	coding (570/918 nt)	oxyR →	oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,159,055	0	A	.	100.0%	300.5 / NA	72	coding (566/918 nt)	oxyR	oxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (36/36); total (36/36)

CGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGC  >  NC_000913/4158918‑4159187

cGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaa                                                                                                                                     <  2:210818/139‑1 (MQ=255)

   gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaaac                                                                                                                                  >  1:1047779/1‑138 (MQ=255)

     tCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaaactg                                                                                                                                <  1:921292/139‑4 (MQ=255)

          gcgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGa                                                                                                                           <  1:845600/138‑1 (MQ=255)

          gcgcTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGa                                                                                                                           >  2:845600/1‑138 (MQ=255)

               ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTgg                                                                                                                     >  2:192481/1‑139 (MQ=255)

               ggTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTgg                                                                                                                     <  2:513300/139‑1 (MQ=255)

                  gAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAg                                                                                                                  <  2:579209/139‑1 (MQ=255)

                          gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                          >  1:1367008/1‑139 (MQ=255)

                          gCGAAGCATTCATTGAAGTGCCGTTGTTTGACGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCAc                                                                                                          >  1:1240117/1‑139 (MQ=255)

                                    cattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAg                                                                                                                  >  1:483326/1‑121 (MQ=255)

                                    cattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAg                                                                                                                  <  2:483326/121‑1 (MQ=255)

                                      ttGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  1:599560/139‑1 (MQ=255)

                                           gTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAActgct                                                                                                                             <  1:632295/103‑1 (MQ=255)

                                           gTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAActgct                                                                                                                             >  2:632295/1‑103 (MQ=255)

                                            tGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  1:372237/133‑1 (MQ=255)

                                            tGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              >  2:372237/1‑133 (MQ=255)

                                             gCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                       >  1:1268584/1‑139 (MQ=255)

                                              ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAt                                                                                      >  1:394469/1‑139 (MQ=255)

                                                    tttGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGttt                                                                                <  2:1367008/139‑1 (MQ=255)

                                                          gaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGttt                                                                          <  2:747958/139‑1 (MQ=255)

                                                           aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAActgct                                                                                                                             <  2:975747/87‑1 (MQ=255)

                                                           aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAActgct                                                                                                                             >  1:975747/1‑87 (MQ=255)

                                                                    tGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCggg                                                                <  1:34771/139‑1 (MQ=255)

                                                                      cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              >  2:356126/1‑107 (MQ=255)

                                                                      cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  1:356126/107‑1 (MQ=255)

                                                                       tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGa                                                                                                                           <  1:8062/77‑1 (MQ=255)

                                                                       tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGa                                                                                                                           >  2:8062/1‑77 (MQ=255)

                                                                         gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                           <  2:394469/139‑1 (MQ=255)

                                                                           tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  2:495897/102‑1 (MQ=255)

                                                                           tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              >  1:495897/1‑102 (MQ=255)

                                                                           tatctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                         <  1:18904/139‑1 (MQ=255)

                                                                            atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑ATAActgctg                                                                                                                            >  1:851228/1‑71 (MQ=255)

                                                                            atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAActgctg                                                                                                                            <  2:851228/71‑1 (MQ=255)

                                                                                   aaGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                 >  1:1305122/1‑139 (MQ=255)

                                                                                    aGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacac                                                >  2:1160655/1‑139 (MQ=255)

                                                                                       tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACt                                             <  1:192481/139‑1 (MQ=255)

                                                                                        cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAATCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              >  1:967600/1‑89 (MQ=255)

                                                                                        cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTTGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  2:967600/89‑1 (MQ=255)

                                                                                        cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACtt                                            >  1:453918/1‑139 (MQ=255)

                                                                                        cACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACtt                                            >  1:436927/1‑139 (MQ=255)

                                                                                         aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                           >  1:568822/1‑139 (MQ=255)

                                                                                         aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                           >  2:410051/1‑139 (MQ=255)

                                                                                         aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                           >  2:259277/1‑139 (MQ=255)

                                                                                         aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGCCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTc                                           >  1:282322/1‑139 (MQ=255)

                                                                                           ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                       >  1:1056727/1‑93 (MQ=255)

                                                                                           ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCaa                                                                                       <  2:1056727/93‑1 (MQ=255)

                                                                                              tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgcgc                                      >  2:1076494/1‑139 (MQ=255)

                                                                                                ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              <  1:404778/81‑1 (MQ=255)

                                                                                                ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                              >  2:404778/1‑81 (MQ=255)

                                                                                                ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                               <  1:1321592/112‑1 (MQ=255)

                                                                                                ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                               >  2:1321592/1‑112 (MQ=255)

                                                                                                 gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAc                                   <  2:282322/139‑1 (MQ=255)

                                                                                                    aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                <  2:568822/139‑1 (MQ=255)

                                                                                                     aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                               <  2:714871/107‑1 (MQ=255)

                                                                                                     aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                               >  1:714871/1‑107 (MQ=255)

                                                                                                     aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                               >  1:822212/1‑139 (MQ=255)

                                                                                                     aCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                               >  2:587281/1‑139 (MQ=255)

                                                                                                         cgAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTgg                           <  2:1305122/139‑1 (MQ=255)

                                                                                                         cgAATGCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTgg                           >  2:369844/1‑139 (MQ=255)

                                                                                                              gCGTACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                      <  2:822212/139‑1 (MQ=255)

                                                                                                                 tACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATgtt                                                                                   >  1:1304647/1‑73 (MQ=255)

                                                                                                                 tACCGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATgtt                                                                                   <  2:1304647/75‑3 (MQ=255)

                                                                                                                   ccGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                 <  1:407239/139‑1 (MQ=255)

                                                                                                                   ccGATGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                 <  2:1047779/139‑1 (MQ=255)

                                                                                                                      aTGGCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa              >  2:1122843/1‑139 (MQ=255)

                                                                                                                         gCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                         >  1:769728/1‑93 (MQ=255)

                                                                                                                         gCCGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATg                                                         <  2:769728/93‑1 (MQ=255)

                                                                                                                           cGATCTGGCAGGGG‑AAATCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATTGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg         <  1:410051/139‑1 (MQ=255)

                                                                                                                           cGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGa                                                                                                                 >  1:1365437/1‑35 (MQ=37)

                                                                                                                           cGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGa                                                                                                                 <  2:1365437/35‑1 (MQ=25)

                                                                                                                           cGATCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg         <  1:259277/139‑1 (MQ=255)

                                                                                                                              tCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                <  1:170116/113‑1 (MQ=255)

                                                                                                                              tCTGGCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                >  2:170116/1‑113 (MQ=255)

                                                                                                                                  gCAGGGG‑AAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcggc  <  2:890444/139‑1 (MQ=255)

CGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGC  >  NC_000913/4158918‑4159187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A