Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,375,282	A→G	Y126H (TAT→CAT)	epmB ←	EF‑P‑Lys34 lysylation protein, weak lysine 2,3‑aminomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,375,282	0	A	G	100.0%	171.7 / NA	48	Y126H (TAT→CAT)	epmB	EF‑P‑Lys34 lysylation protein, weak lysine 2,3‑aminomutase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/24); total (24/24)

CAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAA  >  NC_000913/4375154‑4375411

cAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAAttt                                                                                                                         >  1:1254313/1‑139 (MQ=255)

       tCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCgcgc                                                                                                                  >  2:1410451/1‑139 (MQ=255)

          ccgccgGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAgc                                                                                                               <  1:548630/139‑1 (MQ=255)

               ggAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCt                                                                                                          <  2:49936/139‑1 (MQ=255)

                          tctcGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCaa                                                                                               <  2:119599/139‑1 (MQ=255)

                           ctcGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAg                                                                                                                <  1:1221587/121‑1 (MQ=255)

                           ctcGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAg                                                                                                                >  2:1221587/1‑121 (MQ=255)

                               tCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGACAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAgcg                                                                                          >  2:309141/1‑139 (MQ=255)

                                 cAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        <  1:1040355/139‑1 (MQ=255)

                                 cAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        <  2:362082/139‑1 (MQ=255)

                                       cGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTtg                                                                                  <  2:1254313/139‑1 (MQ=255)

                                         gATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCgg                                                                                     >  1:1199482/1‑134 (MQ=255)

                                         gATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCgg                                                                                     <  2:1199482/134‑1 (MQ=255)

                                            gcgcAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTa                                                                             >  2:153639/1‑139 (MQ=255)

                                                      aTACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGcaac                                                                   <  1:808702/139‑1 (MQ=255)

                                                       tACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGcaaca                                                                  >  1:1002515/1‑139 (MQ=255)

                                                       tACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGcaaca                                                                  >  2:1379244/1‑139 (MQ=255)

                                                       tACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGcaaca                                                                  <  1:595993/139‑1 (MQ=255)

                                                           cAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAAcc                                                              <  1:230430/139‑1 (MQ=255)

                                                            aaGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        >  1:496002/1‑112 (MQ=255)

                                                            aaGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        <  2:496002/112‑1 (MQ=255)

                                                              gTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAgg                                                           >  2:1371944/1‑139 (MQ=255)

                                                               tGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGc                                                          >  2:34887/1‑139 (MQ=255)

                                                                gCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTg                                                                                                       >  1:1347942/1‑93 (MQ=255)

                                                                gCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTg                                                                                                       <  2:1347942/93‑1 (MQ=255)

                                                                 cAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGGAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCATCCGCCTTTGACCAGCAAAAGCGCCCGGGTGTTGTATTTATGCAACAAACCAGGCAc                                                        >  2:855007/1‑139 (MQ=255)

                                                                 cAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAAcc                                                              >  1:813893/1‑133 (MQ=255)

                                                                 cAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAAcc                                                              <  2:813893/133‑1 (MQ=255)

                                                                         cAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACgctgt                                                <  1:1379244/139‑1 (MQ=255)

                                                                         cAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACgctgt                                                >  2:237995/1‑139 (MQ=255)

                                                                         cAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACg                                                    <  2:1114773/135‑1 (MQ=255)

                                                                         cAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACg                                                    >  1:1114773/1‑135 (MQ=255)

                                                                                          cTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACgctgtgctg                                            <  2:286334/126‑1 (MQ=255)

                                                                                          cTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACgctgtgctg                                            >  1:286334/1‑126 (MQ=255)

                                                                                          cTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTggg                               >  2:1239730/1‑139 (MQ=255)

                                                                                            gATTTTCGGCATAGGGGAAGTGACGAAGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCGAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTc                             <  1:855007/139‑1 (MQ=255)

                                                                                                       tAGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATc                  >  2:896886/1‑139 (MQ=255)

                                                                                                        aGGGGAAGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATcc                 >  1:1141926/1‑139 (MQ=255)

                                                                                                             aaGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGgcg            <  2:1141926/139‑1 (MQ=255)

                                                                                                             aaGTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGgcg            <  1:1410451/139‑1 (MQ=255)

                                                                                                               gTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCg                                                                                      <  2:21460/63‑1 (MQ=255)

                                                                                                               gTGACGACGGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCg                                                                                      >  1:21460/1‑63 (MQ=255)

                                                                                                                  acgacgGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        <  2:103491/58‑1 (MQ=255)

                                                                                                                  acgacgGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGcc                                                                                        >  1:103491/1‑58 (MQ=255)

                                                                                                                  acgacgGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACg                                                    >  2:179079/1‑94 (MQ=255)

                                                                                                                  acgacgGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACg                                                    <  1:179079/94‑1 (MQ=255)

                                                                                                                     acgGAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACa    >  2:706032/1‑139 (MQ=255)

                                                                                                                       ggAAGCAATGGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACaaa  <  1:237995/139‑1 (MQ=255)

CAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAA  >  NC_000913/4375154‑4375411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A