Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I2 R1	758	59.3	2864096	93.9%	2689386	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,538	C→T	213 (TAG→TAA)	msrA ←	methionine sulfoxide reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,538	0	C	T	100.0%	256.1 / NA	78	213 (TAG→TAA)	msrA	methionine sulfoxide reductase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (39/39); total (39/39)

AGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGAT  >  NC_000913/4441414‑4441669

aGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCcggcgg                                                                                                                       >  1:912301/1‑139 (MQ=255)

       cGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGcagacag                                                                                                                >  1:565987/1‑139 (MQ=255)

            tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTc                                                                                                                             >  1:218517/1‑121 (MQ=255)

            tCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTc                                                                                                                             <  2:218517/121‑1 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   >  1:80578/1‑126 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   <  2:80578/126‑1 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   <  1:1163808/126‑1 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   >  2:1163808/1‑126 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcg                                                                                                             <  1:1031067/116‑1 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcg                                                                                                             >  2:1031067/1‑116 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                       <  2:1407400/138‑1 (MQ=255)

                                 ggACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                       >  1:1407400/1‑138 (MQ=255)

                                                 cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                       <  1:1011856/122‑1 (MQ=255)

                                                 cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                              >  2:1243843/1‑131 (MQ=255)

                                                 cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                              <  1:1243843/131‑1 (MQ=255)

                                                 cTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTATATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGt                                                                                       >  2:1011856/1‑122 (MQ=255)

                                                  tGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGTGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     <  2:912301/139‑1 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                              <  2:503858/129‑1 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACg                                                                              >  1:503858/1‑129 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATg                                                                    <  2:969914/139‑1 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATg                                                                    >  1:969914/1‑139 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGACATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   <  2:967931/108‑1 (MQ=255)

                                                   ggTAAGTCGTTTACGCCGCATCAGACATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgc                                                                                                   >  1:967931/1‑108 (MQ=255)

                                                    gTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGc                                                                   >  2:110825/1‑139 (MQ=255)

                                                         tCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     >  1:553299/1‑132 (MQ=255)

                                                         tCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     <  2:553299/132‑1 (MQ=255)

                                                             ttACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGc                                                          <  1:1242281/139‑1 (MQ=255)

                                                                          ggCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                             <  1:371580/139‑1 (MQ=255)

                                                                                 aCAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGc                                                                   >  1:640539/1‑110 (MQ=255)

                                                                                 aCAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGc                                                                   <  2:640539/110‑1 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcac                                                                                          <  2:991944/83‑1 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCcac                                                                                          >  1:991944/1‑83 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                    <  2:626249/121‑1 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtggtgg                                                    >  1:626249/1‑121 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCAtaataa                                  >  2:1045655/1‑139 (MQ=255)

                                                                                     agagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCAtaataa                                  >  2:951249/1‑139 (MQ=255)

                                                                                      gagCACTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaa                                 <  1:110825/139‑1 (MQ=255)

                                                                                           cTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaat                                                                                               >  1:33967/1‑72 (MQ=255)

                                                                                           cTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattccgccaat                                                                                               <  2:33967/72‑1 (MQ=255)

                                                                                           cTTTATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGgt                            >  2:1323645/1‑139 (MQ=255)

                                                                                            tttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattcctc                                                                                                   <  2:568007/67‑3 (MQ=255)

                                                                                            tttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGAcgccaattcctc                                                                                                   >  1:568007/1‑65 (MQ=255)

                                                                                             ttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     <  2:814966/96‑1 (MQ=255)

                                                                                             ttATCAACAAACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     >  1:814966/1‑96 (MQ=255)

                                                                                                 caacaaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                  <  1:210754/95‑1 (MQ=255)

                                                                                                 caacaaACTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                  >  2:210754/1‑95 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                           >  1:1010025/1‑65 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCca                                                                                           <  2:1010025/65‑1 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                  >  2:470705/1‑90 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCa                                                                  <  1:470705/90‑1 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                   <  2:1421879/105‑1 (MQ=255)

                                                                                                      aaCTACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                   >  1:1421879/1‑105 (MQ=255)

                                                                                                         tACATTTGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt              <  1:951249/139‑1 (MQ=255)

                                                                                                              ttGTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCg         <  1:1045655/139‑1 (MQ=255)

                                                                                                                gTACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCgtg       <  1:1323645/139‑1 (MQ=255)

                                                                                                                 tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtgg                                                       >  1:450244/1‑90 (MQ=255)

                                                                                                                 tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCtgg                                                       <  2:450244/90‑1 (MQ=255)

                                                                                                                 tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          <  2:1289689/103‑1 (MQ=255)

                                                                                                                 tACCCGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          >  1:1289689/1‑103 (MQ=255)

                                                                                                                   cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          <  2:683148/101‑1 (MQ=255)

                                                                                                                   cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          >  1:511372/1‑101 (MQ=255)

                                                                                                                   cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          >  1:683148/1‑101 (MQ=255)

                                                                                                                   cccGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCgg                                          <  2:511372/101‑1 (MQ=255)

                                                                                                                     cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCgtggt     <  1:820563/136‑1 (MQ=255)

                                                                                                                     cGTAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGAt  >  1:1175404/1‑139 (MQ=255)

                                                                                                                     cGTAACGTTATGCTTCCAGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAGGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCgtggt     >  2:820563/1‑136 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     <  1:167853/70‑1 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     >  2:761817/1‑70 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     <  1:761817/70‑1 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTAt                                                                     >  2:167853/1‑70 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt              <  2:1418473/125‑1 (MQ=255)

                                                                                                                       tAACGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGAt              >  1:1418473/1‑125 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                   >  2:268560/1‑87 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTggtggt                                                   <  1:268560/87‑1 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                             >  1:863416/1‑93 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCtt                                             <  2:863416/93‑1 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATaaa                                 <  1:1021432/105‑1 (MQ=255)

                                                                                                                        aaCGTTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATAGTGCTGGTGGTCATCTTCGGCATAATaaa                                 >  2:1021432/1‑105 (MQ=255)

AGGCCTACGCAGTCCTTGCAATATATTGAATTTGGACGATTTTGTAGGCCTGGTAAGTCGTTTACGCCGCATCAGGCATAGACAAAGAGCACTTTATCAACAAACTACATTTGTACCCGTAACGCTATGCTTCCGGCGGCAGACAGACGCCAATTCCGCCAATTCCACAGTAACCATACGGGTTTTTATGCAGATATTGCTGGTGGTCATCTTCGGCATAATAAAACGGTGTGGCGTTAGCGATTTCCGTGGTGAT  >  NC_000913/4441414‑4441669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A