Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
631,812 |
T→C |
F640S (TTC→TCC) |
cstA → |
carbon starvation protein involved in peptide utilization, APC peptide transporter family protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 631,812 | 0 | T | C | 100.0%
| 97.7
/ NA
| 28 | F640S (TTC→TCC) | cstA | carbon starvation protein involved in peptide utilization, APC peptide transporter family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/14); total (14/14) |
AGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAAC > NC_000913/631681‑631935
|
aGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCaacaac < 1:356242/139‑1 (MQ=255)
tttAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGt < 1:443094/139‑1 (MQ=255)
ggATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCcaaca < 2:840848/113‑1 (MQ=255)
ggATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCcaaca > 1:840848/1‑113 (MQ=255)
ttCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTtct > 2:598326/1‑139 (MQ=255)
ttCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTtct > 2:38082/1‑139 (MQ=255)
ttCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTATCCAACAACCGTCTGGATGCCGGGTTTACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTtct > 2:80075/1‑139 (MQ=255)
cGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTTACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCg < 1:80075/139‑1 (MQ=255)
cGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCg < 2:36085/139‑1 (MQ=255)
cGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCg > 2:629056/1‑139 (MQ=255)
cGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCg < 1:629056/139‑1 (MQ=255)
tgCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt < 1:467194/79‑1 (MQ=255)
tgCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt > 2:467194/2‑80 (MQ=255)
gCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGc > 1:26597/1‑139 (MQ=255)
tCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt < 1:730008/68‑1 (MQ=255)
tCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt > 2:730008/1‑68 (MQ=255)
cAGTATACTGAGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGa > 1:485125/1‑139 (MQ=255)
aGTCACAGCTGGCGCCACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCgtg < 1:388137/74‑1 (MQ=255)
aGTCACAGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCgtg > 2:388137/1‑74 (MQ=255)
caGCTGGCGCAACTGGTGTCCAACATCCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCgtg < 1:451564/69‑1 (MQ=255)
caGCTGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCgtg > 2:451564/1‑69 (MQ=255)
tGGCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAAc < 2:485125/139‑1 (MQ=255)
ggCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTAtggtg > 2:630543/1‑58 (MQ=255)
ggCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTAtggtg < 1:630543/58‑1 (MQ=255)
ggCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGcc < 2:694454/123‑1 (MQ=255)
ggCGCAACTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGcc > 1:694454/1‑123 (MQ=255)
aCTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt < 2:286926/39‑1 (MQ=37)
aCTGGTGTCCAACAACCGTCTGGATGCCGGGTTAACCAt > 1:286926/1‑39 (MQ=37)
|
AGGCTGGCAGAAAGCGTTTAGCCCGGATGCGAAAGTCGGCTTCCTGGCCATTGCTAATAAGTTCCAGGCAATGATCGACAGCGGCAATATTCCGTCGCAGTATACTGAGTCACAGCTGGCGCAACTGGTGTTCAACAACCGTCTGGATGCCGGGTTAACCATCTTCTTTATGGTGGTCGTGGTGGTTCTGGCACTGTTCTCGATTAAGACGGCACTTGCGGCATTGAAAGATCCGAAGCCAACGGCGAAAGAAAC > NC_000913/631681‑631935
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
