Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,302,066 |
G→A |
R230C (CGT→TGT) |
napA ← |
nitrate reductase, periplasmic, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,302,066 | 0 | G | A | 100.0%
| 111.0
/ NA
| 35 | R230C (CGT→TGT) | napA | nitrate reductase, periplasmic, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/18); total (17/18) |
AGTTGGCGATGTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACGGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAA > NC_000913/2301934‑2302195
|
aGTTGGCGATGTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGt < 1:727524/139‑1 (MQ=255)
ttGGCGATGTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGa < 2:635954/139‑1 (MQ=255)
ggCGATGTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATg > 2:122061/1‑139 (MQ=255)
gTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGAc > 2:444633/1‑139 (MQ=255)
cAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAgg < 1:444633/139‑1 (MQ=255)
cggcgTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATg < 2:462432/106‑1 (MQ=255)
cggcgTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATg > 1:462432/1‑106 (MQ=255)
ggcgTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgc > 1:736445/1‑106 (MQ=255)
ggcgTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgc < 2:736445/106‑1 (MQ=255)
tgatgCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCca < 1:335162/139‑1 (MQ=255)
atgCCGTTATTCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCcaca > 1:407407/1‑139 (MQ=255)
cGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGcac > 2:533414/1‑139 (MQ=255)
tACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGAt < 1:122061/139‑1 (MQ=255)
ggtaggtaGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACaa > 1:490506/1‑111 (MQ=255)
ggtaggtaGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACaa < 2:490506/111‑1 (MQ=255)
gtaggtagAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGAt < 2:554937/129‑1 (MQ=255)
gtaggtagAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGAt > 1:554937/1‑129 (MQ=255)
tagAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgc < 1:324273/54‑1 (MQ=255)
tagAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgc > 2:324273/1‑54 (MQ=255)
gAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCa < 1:242477/73‑1 (MQ=255)
gAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCa > 2:242477/1‑73 (MQ=255)
gAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCa < 1:533414/139‑1 (MQ=255)
aGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgcgc > 1:360888/1‑51 (MQ=255)
aGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATgcgc < 2:360888/51‑1 (MQ=255)
aGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGc > 2:285217/1‑96 (MQ=255)
aGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGc < 1:285217/96‑1 (MQ=255)
gCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCgg < 2:407407/139‑1 (MQ=255)
ccACGGTGACGTTCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTCATc > 2:178738/1‑139 (MQ=255)
ttCTGGTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCa > 2:510303/1‑52 (MQ=255)
ttCTGGTTAGACAGACGACAGTTAGTGATGCGCGACCTGAGGATCGGGTGCa < 1:510303/52‑1 (MQ=255)
gTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCa < 2:286233/113‑1 (MQ=255)
gTTAGAGAGACGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCa > 1:286233/1‑113 (MQ=255)
agagaCGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCgg > 2:420011/1‑112 (MQ=255)
agagaCGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCgg < 1:420011/112‑1 (MQ=255)
agagaCGACAGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATaaa > 1:726147/1‑139 (MQ=255)
cgacaGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTc > 1:597359/1‑110 (MQ=255)
cgacaGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTc < 2:597359/110‑1 (MQ=255)
|
AGTTGGCGATGTAGTTCAGGATCACCAGGTCAGATTGCGGCGTAAAGATGATGCCGTTATCCGCCAGCTCGAAGCTACGATGCTGGTAGGTAGAAAGCACCGCCACGGTGACGTTCTGGTTAGAGAGACGACGGTTAGTGATGCGCGACCAGAGGATCGGGTGCATTTCCGCCATGTTTGCGCCCCACAGCACAAACGCGTCAGCCTGCTCGATGTCGTCATAGCAGCCCATCGGCTCATCCATACCAAAGGTACGCATAAA > NC_000913/2301934‑2302195
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
