Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F1 I1 R1	748	32.0	1685426	92.6%	1560704	103.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,685,910	G→A	A26T (GCC→ACC)	hmp →	fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,685,910	0	G	A	100.0%	114.7 / NA	37	A26T (GCC→ACC)	hmp	fused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/18); total (19/18)

TTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCT  >  NC_000913/2685778‑2686024

ttgtttATAAGATGCATTGGAGATACACCAATTAAGATGGAAAATAAGTAAGACCATGTGCTTGCAGCTCAAACCATCGCTACAGTAAAAGGTAGCATCCCTTTATTGTTGGAAACGGTGCCGATGTTGACGACCCAtt                                                                                                              >  2:291845/4‑139 (MQ=255)

        aaGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                      <  1:570492/139‑1 (MQ=255)

        aaGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                      <  2:580568/139‑1 (MQ=255)

          gATGCATTTGAGATACATCAATTAAGATGCAAAAACAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACGGGTGGAAACGGGGCCAAAGTTAACCACCCATATCTACGACCg                                                                                                    >  2:66326/1‑139 (MQ=255)

          gATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                    >  1:214196/1‑139 (MQ=255)

                tttGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                              <  1:675082/139‑1 (MQ=255)

                        aCATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATa                                                                                      >  2:226751/1‑139 (MQ=255)

                               ttAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                              <  1:433231/124‑1 (MQ=255)

                               ttAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                              >  2:433231/1‑124 (MQ=255)

                                    aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                          >  1:313532/1‑139 (MQ=255)

                                      gCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCaaa                                                                        >  2:380734/1‑139 (MQ=255)

                                        aaaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                        <  1:17415/121‑1 (MQ=255)

                                        aaaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                        >  2:17415/1‑121 (MQ=255)

                                                 aaGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTaa                                                             <  1:380734/139‑1 (MQ=255)

                                                  aGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGt                                                                                                   <  2:789243/100‑1 (MQ=255)

                                                  aGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGt                                                                                                   >  1:789243/1‑100 (MQ=255)

                                                         atGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTa                                                     <  2:481376/139‑1 (MQ=255)

                                                           gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                   >  2:176257/1‑139 (MQ=255)

                                                           gCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                   >  1:838904/1‑139 (MQ=255)

                                                               gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTAc                                                                                                         <  2:704979/81‑1 (MQ=255)

                                                               gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTAc                                                                                                         >  1:704979/1‑81 (MQ=255)

                                                                            tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAaccacc                                                                                                                  >  1:79544/1‑59 (MQ=255)

                                                                            tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAaccacc                                                                                                                  <  2:79544/59‑1 (MQ=255)

                                                                            tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                 >  2:725418/1‑60 (MQ=255)

                                                                            tCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                 <  1:725418/60‑1 (MQ=255)

                                                                              gCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAAc                                <  1:507750/139‑1 (MQ=255)

                                                                                            caccaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGttt                  <  2:838904/139‑1 (MQ=255)

                                                                                             accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACg                                                                                                        >  1:755777/1‑52 (MQ=255)

                                                                                             accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACg                                                                                                        <  2:755777/52‑1 (MQ=255)

                                                                                               caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                   >  2:259839/1‑103 (MQ=255)

                                                                                               caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAAc                                                   <  1:259839/103‑1 (MQ=255)

                                                                                                            gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                              >  2:761270/1‑47 (MQ=255)

                                                                                                            gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                              <  1:761270/47‑1 (MQ=255)

                                                                                                            gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCa                                  >  2:388396/1‑107 (MQ=255)

                                                                                                            gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCa                                  <  1:388396/107‑1 (MQ=255)

                                                                                                            gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt  >  2:647019/1‑139 (MQ=255)

                                                                                                             tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                    >  1:525810/1‑40 (MQ=255)

                                                                                                             tggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                    <  2:525810/40‑1 (MQ=255)

                                                                                                                 aaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACg                               <  1:656229/105‑1 (MQ=255)

                                                                                                                 aaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACg                               >  2:656229/1‑105 (MQ=255)

TTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCT  >  NC_000913/2685778‑2686024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A