Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
748 |
32.0 |
1685426 |
92.6% |
1560704 |
103.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,786,746 |
Δ1 bp |
pseudogene (350/2253 nt) |
ygaQ → |
uncharacterized protein, putative enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,786,746 | 0 | C | . | 100.0%
| 137.6
/ NA
| 32 | pseudogene (350/2253 nt) | ygaQ | uncharacterized protein, putative enzyme |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (16/16); total (16/16) |
TTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAA > NC_000913/2786616‑2786863
|
ttAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGa > 1:808213/1‑139 (MQ=255)
aaaaaaCGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGaaa < 2:80249/139‑1 (MQ=255)
aaTTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg < 1:124581/139‑1 (MQ=255)
ttCCCTTTCTGCAGCTACAAATGACCGAGAGATGAATAAAGCAGCACAGCGTAGCATTACAGCGTGAGACATGATAGGAGCAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAAGAGCGgtg < 2:395420/139‑1 (MQ=255)
tttCTCCAGATACAACTTACCTAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg < 1:567080/131‑1 (MQ=255)
tttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg > 2:567080/1‑131 (MQ=255)
tttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTAt < 2:808213/139‑1 (MQ=255)
tttCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTAt < 1:305201/139‑1 (MQ=255)
tcCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGt > 2:604/1‑139 (MQ=255)
gATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATgg > 1:115677/1‑139 (MQ=255)
cacaGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg > 2:798253/1‑92 (MQ=255)
cacaGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg < 1:1561/92‑1 (MQ=255)
cacaGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg > 2:1561/1‑92 (MQ=255)
cacaGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCg < 1:798253/92‑1 (MQ=255)
tACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGCAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTccc > 2:300120/1‑139 (MQ=255)
gcgcCAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCaaa > 2:434450/1‑139 (MQ=255)
ccAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGaaa > 1:554124/1‑57 (MQ=255)
ccAGACAGGATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGa > 1:351424/1‑139 (MQ=255)
acAGACAGGATAGGAGATAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGaaa < 2:554124/56‑1 (MQ=255)
ggATAGGAGAAAGAAAAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAatat < 1:434450/139‑1 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGaaa > 1:407236/1‑39 (MQ=37)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGaaa < 2:407236/39‑1 (MQ=37)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAg < 2:495060/52‑1 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAg > 1:495060/1‑52 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGgt > 1:138916/1‑56 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGgt < 2:138916/56‑1 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGAt < 1:77097/122‑1 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGAt > 2:77097/1‑122 (MQ=255)
agaaaAACATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGc > 2:220141/1‑139 (MQ=255)
aCATAAGTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTaa < 1:415386/139‑1 (MQ=255)
gTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa < 2:785343/112‑1 (MQ=255)
gTTTATCAACGAGATA‑AATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATaa > 1:785343/1‑112 (MQ=255)
|
TTAAAAAACGATTATAAAATTTCCCTTTCTCCAGATACAACTTACCGAGAGTTGAATAAAGCAGCACAGCGTAGCATTACAGCGCCAGACAGGATAGGAGAAAGAAAAACATAGGTTTATCAACGAGATACAATGATTGAAAGAGGTGATAACAGCGGTGTTTATCAGTATGGTCGTGCTGAACATTTCACCCACATTATATCTGACAAACCTTCCCCAAAAGATAAATATGTTGCATATGCTATTAA > NC_000913/2786616‑2786863
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
