Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 748 32.0 1685426 92.6% 1560704 103.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,787,434 G→A pseudogene (1038/2253 nt) ygaQ → uncharacterized protein, putative enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,787,4340GA100.0% 189.3 / NA 58pseudogene (1038/2253 nt)ygaQuncharacterized protein, putative enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/27);  total (31/27)

TCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAGCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCT  >  NC_000913/2787311‑2787554
                                                                                                                           |                                                                                                                        
tCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTg                                                                                                           >  2:814753/1‑139 (MQ=255)
 ccTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGa                                                                                                          >  2:608430/1‑139 (MQ=255)
    gATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGttt                                                                                                             <  2:4802/133‑1 (MQ=255)
    gATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGttt                                                                                                             >  1:4802/1‑133 (MQ=255)
                              tGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACaaaa                                                                             <  2:436807/139‑1 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         <  2:71589/139‑1 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         <  2:747301/139‑1 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         <  1:202547/139‑1 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         >  2:280173/1‑139 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         <  2:807522/139‑1 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         >  1:807522/1‑139 (MQ=255)
                                                  aCCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAatccatcc                                                         >  1:71589/1‑139 (MQ=255)
                                                            tCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGAtata                                               >  1:244091/1‑139 (MQ=255)
                                                                 ctgctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACAtct                                          >  1:677698/1‑139 (MQ=255)
                                                                 ctgctgATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATACAGAAGATATACAtat                                          >  2:235150/1‑137 (MQ=255)
                                                                       atatACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTa                                                                                              >  2:109361/1‑81 (MQ=255)
                                                                       atatACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTa                                                                                              <  1:109361/81‑1 (MQ=255)
                                                                            cGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATcc                                                                                       <  1:723555/83‑1 (MQ=255)
                                                                            cGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATcc                                                                                       >  2:723555/1‑83 (MQ=255)
                                                                              aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCa                                   >  1:260486/1‑133 (MQ=255)
                                                                              aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCa                                   <  2:260486/133‑1 (MQ=255)
                                                                              aGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGAc                             >  2:92274/1‑139 (MQ=255)
                                                                               gCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAAc                                                                         <  1:568338/94‑1 (MQ=255)
                                                                               gCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAAc                                                                         >  2:568338/1‑94 (MQ=255)
                                                                                  aaGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     >  1:466081/1‑63 (MQ=255)
                                                                                  aaGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     <  2:466081/63‑1 (MQ=255)
                                                                                  aaGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGAt                                                                                         <  1:579875/75‑1 (MQ=255)
                                                                                  aaGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGAt                                                                                         >  2:579875/1‑75 (MQ=255)
                                                                                  aaGATCAGTAAAGATATAGAAGGTGGTATCCCTGACTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCg                         >  2:821538/1‑139 (MQ=255)
                                                                                         gTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     <  1:573090/56‑1 (MQ=255)
                                                                                         gTAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     >  2:573090/1‑56 (MQ=255)
                                                                                          tAAAGATATAGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGt                 <  2:244091/139‑1 (MQ=255)
                                                                                                  taGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCaga                                                      >  1:348882/1‑94 (MQ=255)
                                                                                                  taGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCaga                                                      <  2:348882/94‑1 (MQ=255)
                                                                                                   aGAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        <  1:608430/139‑1 (MQ=255)
                                                                                                    gAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag                                                    >  2:207436/1‑94 (MQ=255)
                                                                                                    gAAGGTGGTATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag                                                    <  1:207436/94‑1 (MQ=255)
                                                                                                        gtggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg                      >  1:829356/1‑120 (MQ=255)
                                                                                                        gtggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAg                      <  2:829356/120‑1 (MQ=255)
                                                                                                        gtggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        >  1:98490/1‑134 (MQ=255)
                                                                                                        gtggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        <  2:98490/134‑1 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt                                                                                                        <  2:339229/37‑1 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGAtt                                                                                                        >  1:339229/1‑37 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     <  2:277602/40‑1 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     >  2:79397/1‑40 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     <  1:79397/40‑1 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCca                                                                                                     >  1:277602/1‑40 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACAt                                            <  2:182148/97‑1 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACAt                                            >  1:182148/1‑97 (MQ=255)
                                                                                                         tggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATctct  >  1:98894/1‑139 (MQ=255)
                                                                                                          ggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        <  2:146949/132‑1 (MQ=255)
                                                                                                          ggtATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        >  1:146949/1‑132 (MQ=255)
                                                                                                            tATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        <  2:388195/130‑1 (MQ=255)
                                                                                                            tATCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAAc        >  1:388195/1‑130 (MQ=255)
                                                                                                              tCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCag                                                       >  2:564870/1‑81 (MQ=255)
                                                                                                              tCCCTGGCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCag                                                       <  1:564870/81‑1 (MQ=255)
                                                                                                                   ggCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag                                                    >  2:616289/1‑79 (MQ=255)
                                                                                                                   ggCTATTAACAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCagaag                                                    <  1:616289/79‑1 (MQ=255)
                                                                                                                           |                                                                                                                        
TCCTGATTATACCTTTAACTTTATGGGAATTGGGGGTAGTGGTAAAAATGACCTGGATTATCCCTCTGCTGATATACGAGCGAAGATCAGTAAAGATATAGAAGGTGGTATCCCTGGCTATTAGCAAGGTCAGGTTTTGATTCCATTCATTAAAGATCCAGTAACAAAAGAACGTAAACAAATCCATCCAGAAGATATACATCTCACTGCAAAAGACTTCGAAGCAAGTAAAGATAACATCTCT  >  NC_000913/2787311‑2787554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A