Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F1 I1 R1 748 32.0 1685426 92.6% 1560704 103.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,239 A→G noncoding (48/87 nt) leuP ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,2390AG100.0% 74.9 / NA 22noncoding (48/87 nt)leuPtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/11);  total (11/11)

ACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC  >  NC_000913/4606121‑4606358
                                                                                                                       |                                                                                                                       
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                                              >  1:246563/1‑130 (MQ=255)
acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACAcc                                                                                                              <  2:246563/130‑1 (MQ=255)
          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGTGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                                                                            >  1:174853/1‑139 (MQ=255)
          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                             >  1:227775/1‑137 (MQ=255)
          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                                             <  2:227775/137‑1 (MQ=255)
                                 cGCCACCTTCGCACAGTCATCTCACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                                                    <  1:628239/139‑1 (MQ=255)
                                   ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                                      >  1:260126/1‑103 (MQ=255)
                                   ccACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                                      <  2:260126/103‑1 (MQ=255)
                                     aCCGTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACTCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                <  1:543175/139‑1 (MQ=255)
                                        ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGTGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCa                                                              <  2:174853/139‑1 (MQ=255)
                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGCGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                                   >  2:511972/1‑92 (MQ=255)
                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGCGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                                   <  1:511972/92‑1 (MQ=255)
                                                           ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                                     >  2:812925/1‑80 (MQ=255)
                                                           ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                                     <  1:812925/80‑1 (MQ=255)
                                                           ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                         >  1:79567/1‑108 (MQ=255)
                                                           ttttttGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                         <  2:79567/108‑1 (MQ=255)
                                                                                  tGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGTGAGCAATATCAACGTGGTTTTTCGTGCGAGGGGCGGGCCTTGAAcccc                   >  2:740895/1‑139 (MQ=18)
                                                                                                    gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                       <  2:392676/102‑1 (MQ=255)
                                                                                                    gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                       >  1:392676/1‑102 (MQ=255)
                                                                                                    gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGTCCGTAAGGacac  >  1:523252/1‑139 (MQ=255)
                                                                                                         cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                           <  2:30137/77‑1 (MQ=255)
                                                                                                         cccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                           >  1:30137/1‑77 (MQ=255)
                                                                                                                       |                                                                                                                       
ACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACAC  >  NC_000913/4606121‑4606358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A