Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	406,492	T→C	F30L (TTT→CTT)	aroL →	shikimate kinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	406,492	0	T	C	100.0%	179.2 / NA	50	F30L (TTT→CTT)	aroL	shikimate kinase II
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/23); total (27/23)

ATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCAC  >  NC_000913/406358‑406622

aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                >  2:223690/1‑139 (MQ=255)

aTGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                >  1:994166/1‑139 (MQ=255)

 tGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTc                                                                                                                               >  1:96335/1‑139 (MQ=255)

 tGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTc                                                                                                                               >  1:460592/1‑139 (MQ=255)

     acCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATa                                                                                                                           >  1:875534/1‑139 (MQ=255)

       cGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCg                                                                                                                              <  1:1036252/134‑1 (MQ=255)

       cGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCg                                                                                                                              >  2:1036252/1‑134 (MQ=255)

       cGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATAcc                                                                                                                         >  2:521313/1‑139 (MQ=255)

                        cGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATc                                                                                                        <  2:994166/139‑1 (MQ=255)

                                       aaCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGAc                                                                                         <  1:1115802/139‑1 (MQ=255)

                                        aCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        <  2:452922/139‑1 (MQ=255)

                                        aCCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        <  1:223690/139‑1 (MQ=255)

                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACgg                                                                                       >  2:896135/1‑139 (MQ=255)

                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        <  2:779180/138‑1 (MQ=255)

                                         cccACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        >  1:779180/1‑138 (MQ=255)

                                               aTGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCgg                                                                                 <  1:521313/139‑1 (MQ=255)

                                                   cacaACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAt                                                                             >  2:121760/1‑139 (MQ=255)

                                                      aaCCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGAtcgt                                                                          <  2:496143/139‑1 (MQ=255)

                                                                    aTCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGt                                                            >  2:664632/1‑139 (MQ=255)

                                                                         gCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCgg                                                                                 >  2:961794/1‑113 (MQ=255)

                                                                         gCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCgg                                                                                 <  1:961794/113‑1 (MQ=255)

                                                                               gggCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGAttt                                                 <  2:96335/139‑1 (MQ=255)

                                                                                     tgGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGcc                                           >  1:492499/1‑139 (MQ=255)

                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                <  1:231262/44‑1 (MQ=255)

                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGt                                                                                                                                >  2:231262/1‑44 (MQ=255)

                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAAt                                                                                                         <  1:216337/67‑1 (MQ=255)

                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAAt                                                                                                         >  2:216337/1‑67 (MQ=255)

                                                                                               acGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcgg                                 >  2:647739/1‑139 (MQ=255)

                                                                                                 ggTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAg                                                                                                    <  2:789025/70‑1 (MQ=255)

                                                                                                 ggTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAg                                                                                                    >  1:789025/1‑70 (MQ=255)

                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAatat                                                                                            <  2:1113082/77‑1 (MQ=255)

                                                                                                  gTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAatat                                                                                            >  1:1113082/1‑77 (MQ=255)

                                                                                                       aaTGGCCCTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTg                         <  1:896135/139‑1 (MQ=255)

                                                                                                            cccTTGCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGc                    >  1:504645/1‑139 (MQ=255)

                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGc                                                                                                            <  2:363643/46‑1 (MQ=255)

                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGc                                                                                                            >  1:363643/1‑46 (MQ=255)

                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTaa               >  2:345035/1‑139 (MQ=255)

                                                                                                                 gCCGATTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTaa               <  2:460592/139‑1 (MQ=255)

                                                                                                                     aTTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa                                                                                          <  2:1059579/60‑1 (MQ=255)

                                                                                                                     aTTCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGa                                                                                          >  1:1059579/1‑60 (MQ=255)

                                                                                                                      ttCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        <  2:167609/61‑1 (MQ=255)

                                                                                                                      ttCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACg                                                                                        >  1:167609/1‑61 (MQ=255)

                                                                                                                      ttCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcggc                                >  2:376403/1‑117 (MQ=255)

                                                                                                                      ttCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAAcggc                                <  1:376403/117‑1 (MQ=255)

                                                                                                                      ttCGCTTAACCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTgcg          <  1:121760/139‑1 (MQ=255)

                                                                                                                              aCCGTCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCAc  >  2:230337/1‑139 (MQ=255)

                                                                                                                                  tCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTTGAAg                     <  2:659808/116‑1 (MQ=255)

                                                                                                                                  tCGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAg                     >  1:659808/1‑116 (MQ=255)

                                                                                                                                   cGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGAttt                                                 >  2:395210/1‑87 (MQ=255)

                                                                                                                                   cGGCTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGAttt                                                 <  1:395210/87‑1 (MQ=255)

ATGACACCGGCTTTCGCCGCATTGCGACCTATTGGGGAAAACCCACGATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCAC  >  NC_000913/406358‑406622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A