Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
634,746 |
2 bp→AT |
|
[ybdL]–[ybdM] |
[ybdL], [ybdM] |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 634,746 | 0 | G | A | 100.0%
| 77.0
/ NA
| 25 | *387* (TAG→TAA) | ybdL | methionine aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/14); total (11/14) |
| * | NC_000913 | 634,747 | 0 | C | T | 100.0%
| 77.9
/ NA
| 25 | *210* (TAG→TAA) | ybdM | Spo0J family protein, ParB‑like nuclease domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/14); total (11/14) |
TAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCC > NC_000913/634625‑634874
||
tAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAgg < 2:856983/139‑1 (MQ=255)
aTTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcg < 2:680497/133‑1 (MQ=255)
aTTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGgcg > 1:680497/1‑133 (MQ=255)
aTTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGaa > 1:1099661/1‑139 (MQ=255)
gtTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGc > 1:710860/1‑139 (MQ=255)
ttttGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCa < 1:399776/139‑1 (MQ=255)
cgcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaacaa < 2:272107/139‑1 (MQ=255)
cgcCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAaacaa < 2:1099661/139‑1 (MQ=255)
aTAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTaa > 2:788610/1‑139 (MQ=255)
aCTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTg > 2:1105488/1‑139 (MQ=255)
tCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTaa < 1:966166/75‑1 (MQ=255)
tCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTaa > 2:966166/1‑75 (MQ=255)
tCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGa > 2:1111659/1‑98 (MQ=255)
tCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGa < 1:1111659/98‑1 (MQ=255)
tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGt < 2:114523/67‑1 (MQ=255)
tGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGt > 1:114523/1‑67 (MQ=255)
gAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTcc < 1:1105488/139‑1 (MQ=255)
tGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTcc > 1:705953/1‑129 (MQ=255)
tGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTcc < 2:705953/129‑1 (MQ=255)
gCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAgg < 1:46981/51‑1 (MQ=255)
gCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAATTATTTAACCGTCCAgg > 2:46981/1‑51 (MQ=255)
aaCGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAgg < 2:110156/93‑1 (MQ=255)
aaCGCCTGCGCCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAgg > 1:110156/1‑93 (MQ=255)
gcgcCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGtc < 1:627827/139‑1 (MQ=255)
gcCAGCTTTAATTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGtccc < 1:788610/139‑1 (MQ=255)
||
TAGCGGCGATTCCGCTGTCGGTGTTTTGCGCCGATCCCTTCCCACATAAACTGATTCGTCTCTGTTTTGCCAAGAAGGAATCGACGTTGCTGGCAGCAGCTGAACGCCTGCGCCAGCTTTAGCTATTTAACCGTCCAGGCGCGGGAATATTGACGGTCGGCAAACAACTCTTGCAGGCCGTTAATTTGCTTCAGGCGCAGCACTTCGTCGCTGTCCATTCCCAGCTCTTTGCCAATTTTATTATCGTCCC > NC_000913/634625‑634874
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
