Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,649,041 |
G→A |
*73* (TAG→TAA) |
ydfC → |
uncharacterized protein, Qin prophage |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,649,041 | 0 | G | A | 100.0%
| 134.7
/ NA
| 43 | *73* (TAG→TAA) | ydfC | uncharacterized protein, Qin prophage |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/19); total (24/19) |
GGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCA > NC_000913/1648924‑1649174
|
ggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGTAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa < 2:1106523/124‑1 (MQ=255)
ggCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa > 1:1106523/1‑124 (MQ=255)
gCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTtaatag > 2:145558/1‑120 (MQ=255)
gCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTtaatag < 1:145558/120‑1 (MQ=255)
tAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt > 1:936026/1‑139 (MQ=255)
tAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGtt < 1:516343/139‑1 (MQ=255)
gCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAAtt > 2:187267/1‑139 (MQ=255)
gCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa > 2:338858/1‑108 (MQ=255)
gCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa < 1:338858/108‑1 (MQ=255)
aGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCt < 2:245873/116‑1 (MQ=255)
aGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCt > 1:245873/1‑116 (MQ=255)
aGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTtct > 2:165424/1‑139 (MQ=255)
aGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTtct > 2:63001/1‑139 (MQ=255)
gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa < 1:102422/99‑1 (MQ=255)
gCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTa > 2:102422/1‑99 (MQ=255)
tGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACggg < 2:936026/139‑1 (MQ=255)
cGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTaa < 1:270170/99‑1 (MQ=255)
cGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTaa > 2:270170/1‑99 (MQ=255)
ctctGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAgtgcg < 1:165424/139‑1 (MQ=255)
tatCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTtaatag < 1:115433/67‑1 (MQ=255)
tatCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTtaatag > 2:115433/1‑67 (MQ=255)
tCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAg > 1:431320/1‑99 (MQ=255)
tCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAg < 2:431320/99‑1 (MQ=255)
cACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt < 2:969874/110‑1 (MQ=255)
cACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt > 1:969874/1‑110 (MQ=255)
aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAg > 1:377777/1‑93 (MQ=255)
aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAg < 2:377777/93‑1 (MQ=255)
aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt < 1:203574/105‑1 (MQ=255)
aTCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGt > 2:203574/1‑105 (MQ=255)
tCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATcaac < 1:389009/67‑1 (MQ=255)
tCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATcaac > 2:389009/1‑67 (MQ=255)
cagcGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGAc > 2:88232/1‑139 (MQ=255)
ttAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGaca > 1:1041983/1‑109 (MQ=255)
ttAAGCATTGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGaca < 2:1041983/109‑1 (MQ=255)
tGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGGGTTCTCCACgg < 2:294311/75‑1 (MQ=255)
tGCCAGAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGGGTTCTCCACgg > 1:294311/1‑75 (MQ=255)
gAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAc < 1:724688/68‑1 (MQ=255)
gAATGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCAc > 2:724688/1‑68 (MQ=255)
aTGATTACATACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTccc > 1:863330/1‑139 (MQ=255)
tACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt < 2:163218/96‑1 (MQ=255)
tACCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCAt > 1:163218/1‑96 (MQ=255)
aCCCGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCa > 2:1085746/1‑139 (MQ=255)
ccGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGaa < 2:411133/115‑1 (MQ=255)
ccGCGTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGaa > 1:411133/1‑115 (MQ=255)
gcgTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGac < 2:1109893/79‑1 (MQ=255)
gcgTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGac > 1:1109893/1‑79 (MQ=255)
cgTTAATAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCa > 2:1025321/1‑139 (MQ=255)
|
GGCTAATGAAGCCAGGGCTAAGCAGGCTGAAAATCTGTCCGCTCTGGAGCAGGATATCACCAATCTGGAAAAAGCAGCGGCATTAAGCATTGCCAGAATGATTACATACCCGCGTTAGTAGCTAATCAACAAAGCTAAGGTTAGTAATTAAGGAGTTCTCCACGGGTGAGGTGGAGTGCGTGCGCCGGACACGGGTGCGCATCCGGAACTGACAGTTTACTGAAAGGATATTTCCCTGAAAAGTCAGACCA > NC_000913/1648924‑1649174
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
