Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 765 46.1 2269152 93.8% 2128464 105.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,942,993 G→A G111E (GGG→GAG)  znuC → zinc ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,942,9930GA100.0% 180.5 / NA 55G111E (GGG→GAG) znuCzinc ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

GGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAA  >  NC_000913/1942869‑1943121
                                                                                                                            |                                                                                                                                
ggCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACg                                                                                                                    <  1:1056737/139‑1 (MQ=255)
  cTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCa                                                                                                                  >  2:811820/1‑139 (MQ=255)
                         tcGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCtggtgg                                                                                           >  2:470977/1‑139 (MQ=255)
                                 acGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                                   <  1:470977/139‑1 (MQ=255)
                                  cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGATCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  2:530475/139‑1 (MQ=255)
                                  cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  1:659907/139‑1 (MQ=255)
                                  cGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGATTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  2:109083/139‑1 (MQ=255)
                                   gTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGctg                                                                                 <  1:811820/139‑3 (MQ=255)
                                    ttGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc                                                                                <  2:499004/139‑1 (MQ=255)
                                       ccACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctctc                                                                                                 >  1:331157/1‑119 (MQ=255)
                                       ccACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGctctc                                                                                                 <  2:331157/119‑1 (MQ=255)
                                       ccACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                             <  2:208774/139‑1 (MQ=255)
                                          cTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTAc                                                                          >  1:1014205/1‑139 (MQ=255)
                                               cGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCCGGTGGCGAAACGCAGCGTGTACTATTa                                                                     >  2:888300/1‑139 (MQ=255)
                                                      cGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                              <  1:148383/139‑1 (MQ=255)
                                                      cGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCCGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                              <  1:888300/139‑1 (MQ=255)
                                                         tttttACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCAt                                                           >  2:595772/1‑139 (MQ=255)
                                                                  ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                                      >  1:201320/1‑87 (MQ=255)
                                                                  ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAg                                                                                                      <  2:201320/87‑1 (MQ=255)
                                                                  ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                    >  2:844093/1‑105 (MQ=255)
                                                                  ttacgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACg                                                                                    <  1:844093/105‑1 (MQ=255)
                                                                     cgcCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGcc                                               <  2:1014205/139‑1 (MQ=255)
                                                                            gTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  2:1093404/97‑1 (MQ=255)
                                                                            gTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  >  1:1093404/1‑97 (MQ=255)
                                                                                  aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  2:1003266/91‑1 (MQ=255)
                                                                                  aTAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  >  1:1003266/1‑91 (MQ=255)
                                                                                    aaaGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  >  2:957491/1‑89 (MQ=255)
                                                                                    aaaGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCa                                                                                  <  1:957491/89‑1 (MQ=255)
                                                                                      agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg                                                                               >  1:113721/1‑90 (MQ=255)
                                                                                      agaagaTATTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCg                                                                               <  2:113721/90‑1 (MQ=255)
                                                                                           atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaa                                                                                                         <  2:257645/59‑1 (MQ=255)
                                                                                           atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCaa                                                                                                         >  1:257645/1‑59 (MQ=255)
                                                                                           atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                              <  1:843442/102‑1 (MQ=255)
                                                                                           atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAg                                                              >  2:843442/1‑102 (MQ=255)
                                                                                           atatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGa                         >  2:112174/1‑139 (MQ=255)
                                                                                            tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                 >  2:227788/1‑82 (MQ=255)
                                                                                            tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                 <  1:227788/82‑1 (MQ=255)
                                                                                            tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                             >  2:229233/1‑86 (MQ=255)
                                                                                            tatTTTGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCgtg                                                                             <  1:229233/86‑1 (MQ=255)
                                                                                              ttttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                 <  1:723780/80‑1 (MQ=255)
                                                                                              ttttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAg                                                                                 >  2:723780/1‑80 (MQ=255)
                                                                                               tttGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAAccc                     >  1:207816/1‑139 (MQ=255)
                                                                                                 tGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                                   <  1:395871/75‑1 (MQ=255)
                                                                                                 tGCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGc                                                                                   >  2:395871/1‑75 (MQ=255)
                                                                                                  gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc                                                                                >  2:147353/1‑77 (MQ=255)
                                                                                                  gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGc                                                                                <  1:147353/77‑1 (MQ=255)
                                                                                                  gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAgcgc                                                                 <  2:345918/92‑1 (MQ=255)
                                                                                                  gCCTGCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAgcgc                                                                 >  1:345918/1‑92 (MQ=255)
                                                                                                    aggCACTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCa                <  1:338789/137‑1 (MQ=255)
                                                                                                         cTGAAACGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCg           <  2:207816/139‑1 (MQ=255)
                                                                                                            aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                                >  1:171934/1‑99 (MQ=255)
                                                                                                            aaaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGc                                                <  2:171934/99‑1 (MQ=255)
                                                                                                             aaCGTGTCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGa       <  1:112174/139‑1 (MQ=255)
                                                                                                                 tgtCCAGGCCGAGCCCCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCCTTGTTAAATCGCCCGCAAGTATTAGGTCTGGAAGAACATACACAAGGCGTGGATgct   >  2:338789/1‑137 (MQ=255)
                                                                                                                  gtCCAGGCCGAGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTaa  >  1:303180/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                
GGCTATGTACCGCAGAAGCTGTATCTCGACACCACGTTGCCACTGACCGTAAACCGTTTTTTACGCTTACGCCCTGGTACACATAAAGAAGATATTTTGCCTGCACTGAAACGTGTCCAGGCCGGGCACCTGATTAACGCACCGATGCAAAAGCTCTCTGGTGGCGAAACGCAGCGTGTACTATTAGCGCGAGCATTGTTAAATCGCCCGCAATTATTAGTGCTGGATGAACCCACTCAAGGCGTGGATGTAA  >  NC_000913/1942869‑1943121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A