Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,031,071	G→A	P416L (CCG→CTG)	dcm ←	DNA cytosine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,031,071	0	G	A	100.0%	67.7 / NA	22	P416L (CCG→CTG)	dcm	DNA cytosine methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (9/13); total (9/13)

ACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAA  >  NC_000913/2030935‑2031197

aCGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAgc                                                                                                                              <  2:599338/139‑1 (MQ=255)

                          ttGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCa                                                                                                    >  1:790632/1‑139 (MQ=255)

                                       ttttGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       <  1:116585/107‑1 (MQ=255)

                                       ttttGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       >  2:116585/1‑107 (MQ=255)

                                                 gcgGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTg                                                                             >  1:355595/1‑139 (MQ=255)

                                                   ggCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCCAGGCGTTAACCGACGTGGa                                                                           <  1:755214/139‑1 (MQ=255)

                                                            cGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGc                                                                  <  2:355595/139‑1 (MQ=255)

                                                            cGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTATCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGc                                                                  <  1:707972/139‑1 (MQ=255)

                                                                            ttACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAgcgcgcg                                                                                                      >  1:153497/1‑87 (MQ=255)

                                                                            ttACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAgcgcgcg                                                                                                      <  2:153497/87‑1 (MQ=255)

                                                                              aCCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       <  1:431977/68‑1 (MQ=255)

                                                                              aCCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       >  2:431977/1‑68 (MQ=255)

                                                                              aCCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCa                                                <  2:790632/139‑1 (MQ=255)

                                                                                 gAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAg                                             <  1:663090/139‑1 (MQ=255)

                                                                                 gAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAg                                             <  1:542287/139‑1 (MQ=255)

                                                                                 gAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAg                                             <  1:360214/139‑1 (MQ=255)

                                                                                                   aGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAgcg                                                                                                                             <  2:34886/41‑1 (MQ=255)

                                                                                                   aGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCAgcg                                                                                                                             >  1:34886/1‑41 (MQ=255)

                                                                                                       tCCGAAACCGGAATACGGAATTTCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCatat                       >  1:587043/1‑139 (MQ=255)

                                                                                                               cGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       >  2:183889/1‑35 (MQ=255)

                                                                                                               cGGAATACGGAATTTCGCTTCTCCCAGCGCTTCaa                                                                                                                       <  1:183889/35‑1 (MQ=255)

                                                                                                                            ttCGCTTCTCCCAGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGTCATATCCCAGCCGCGATCGATTaaaa  >  2:1078282/1‑139 (MQ=255)

ACGCAACGCCACCGCCTGTTTGATTTTTGGCTCAAGCAGTTTTGCCACCGCGGCAAAGACCGGCACGACCACCGAGTTACCGAACTGGCGATAGGCCTGAGTGTCCGAAACCGGAATACGGAATTTCGCTTCTCCCGGCGCTTCAAAACCCATTAAGCGCGCGCATTCCCGAGGCGTTAACCGACGTGGACGATGTTGCTGATTCAGCGGATCGTCAAAGTCTTTCTCACCCGTGGCCATATCCCAGCCGCGATCGATTAAAA  >  NC_000913/2030935‑2031197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A