Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,139,759	C→T	618 (TAG→TAA)	asmA ←	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,139,759	0	C	T	100.0%	182.5 / NA	56	618 (TAG→TAA)	asmA	suppressor of OmpF assembly mutants, putative outer membrane protein assembly factor, inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (27/29); total (27/29)

CGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGG  >  NC_000913/2139632‑2139888

cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGGACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               >  2:663889/1‑132 (MQ=255)

cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               <  1:663889/132‑1 (MQ=255)

cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                        >  1:1032170/1‑139 (MQ=255)

cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                        >  1:306380/1‑139 (MQ=255)

cGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTtctc                                                                                                                        >  2:878614/1‑139 (MQ=255)

     aaCATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCa                                                                                                                   <  1:744660/139‑1 (MQ=255)

         tCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActt                                                                                                               >  2:785925/1‑139 (MQ=255)

                            ttCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTa                                                                                            <  2:776381/139‑1 (MQ=255)

                              cGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                                          <  1:878614/139‑1 (MQ=255)

                                aTCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  2:1032170/139‑1 (MQ=255)

                                    tACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATc                                                                                    >  1:134542/1‑139 (MQ=255)

                                           tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  2:772578/128‑1 (MQ=255)

                                           tCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        >  1:772578/1‑128 (MQ=255)

                                               aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        >  2:505603/1‑124 (MQ=255)

                                               aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  1:505603/124‑1 (MQ=255)

                                               aaTATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCt                                                                         <  2:134542/139‑1 (MQ=255)

                                                atatAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTcaca                                                                                                        >  1:579977/1‑107 (MQ=255)

                                                atatAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTcaca                                                                                                        <  2:579977/107‑1 (MQ=255)

                                                    aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACAtct                                                                                                                            <  2:439957/83‑1 (MQ=255)

                                                    aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACAtct                                                                                                                            >  1:439957/1‑83 (MQ=255)

                                                    aaTGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGcc                                                                    >  2:172303/1‑139 (MQ=255)

                                                     atgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGccc                                                                   <  2:828319/139‑1 (MQ=255)

                                                      tgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                   >  2:83143/1‑106 (MQ=255)

                                                      tgatgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCttt                                                                                                   <  1:83143/106‑1 (MQ=255)

                                                         tgtcgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                                          <  1:975284/109‑1 (MQ=255)

                                                         tgtcgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACg                                                                                          >  2:975284/4‑112 (MQ=255)

                                                         tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActtct                                                                                                             <  2:929753/93‑1 (MQ=255)

                                                         tgCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAActtct                                                                                                             >  1:929753/1‑93 (MQ=255)

                                                           ccgccACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCAt                                                             >  1:748774/1‑139 (MQ=255)

                                                                           aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               <  2:778608/57‑1 (MQ=255)

                                                                           aTGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACa                                                                                                                               >  1:778608/1‑57 (MQ=255)

                                                                               gACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                         <  1:172303/139‑1 (MQ=255)

                                                                               gACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCg                                         <  2:748774/139‑1 (MQ=255)

                                                                                 cGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTc                                       <  1:785925/139‑1 (MQ=255)

                                                                                            tCTTATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGtt                            <  2:306380/139‑1 (MQ=255)

                                                                                              ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGcc                                                                    >  1:586010/1‑97 (MQ=255)

                                                                                              ttATCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGcc                                                                    <  2:586010/97‑1 (MQ=255)

                                                                                                aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAAt                                                                                     <  2:1133979/78‑1 (MQ=255)

                                                                                                aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAAt                                                                                     >  1:1133979/1‑78 (MQ=255)

                                                                                                aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCCGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                        >  1:862422/1‑139 (MQ=255)

                                                                                                aTCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcg                        >  1:755054/1‑139 (MQ=255)

                                                                                                 tCAGGCCTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTgcgc                       <  2:755054/139‑1 (MQ=255)

                                                                                                       cTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAAc                 >  2:388623/1‑139 (MQ=255)

                                                                                                       cTACAGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAAc                 >  2:935402/1‑139 (MQ=255)

                                                                                                           aGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACttcttc                                                                                                           >  1:104544/1‑45 (MQ=255)

                                                                                                           aGGACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACttcttc                                                                                                           <  2:104544/45‑1 (MQ=255)

                                                                                                            ggACTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATc            <  1:388623/139‑1 (MQ=255)

                                                                                                               cTGGTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCAc         >  2:163653/1‑139 (MQ=255)

                                                                                                                  gTCACCTGGTAACTTACATCTTCTCCAGCGACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGtt                            <  2:977477/117‑1 (MQ=255)

                                                                                                                  gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGtt                            >  1:977477/1‑117 (MQ=255)

                                                                                                                  gTCACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTg      <  1:163653/139‑1 (MQ=255)

                                                                                                                    cACCTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCa    <  1:935402/139‑1 (MQ=255)

                                                                                                                       cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAgg  <  2:29576/138‑1 (MQ=255)

                                                                                                                       cTGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAgg  >  1:29576/1‑138 (MQ=255)

                                                                                                                        tGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        <  2:1130865/51‑1 (MQ=255)

                                                                                                                        tGGTAACTTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACggg                                                                                        >  1:1130865/1‑51 (MQ=255)

CGAGGAACATCAAGTGAACGGAACAGCCTTCGATCTTACATATTCAGAATATAATGATGCCGCCACGAACGCCTGATGCGACGCTTTACGCGTCTTATCAGGCCTACAGGACTGGTCACCTGGTAACCTACATCTTCTCCAGCAACTTCTTCACATCTTTGCCATTACGGGAATCTTTATTCCGCTCGGCCCAGTCATTCAGGCGACGTTTCGCTTCGTCCTGTAGATGTTTGCGCAGTAACTGATCCACTTGCAGG  >  NC_000913/2139632‑2139888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A