Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	765	46.1	2269152	93.8%	2128464	105.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,508,659	G→A	G256G (GGC→GGT)	glk ←	glucokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,508,659	0	G	A	100.0%	104.2 / NA	33	G256G (GGC→GGT)	glk	glucokinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/15); total (18/15)

ACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCGCCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGTTCGGTAATATCTTTTGGCTTGAG  >  NC_000913/2508528‑2508790

aCGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATg                                                                                                                              <  2:471991/139‑1 (MQ=255)

    tGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCTCCCGCAATAAACACGCCACCAAATGTccc                                                                                                                          >  1:768473/1‑139 (MQ=255)

    tGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTccc                                                                                                                          >  1:892300/1‑139 (MQ=255)

              cGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAgc                                                                                                                >  2:980881/1‑139 (MQ=255)

                gAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAgcgc                                                                                                              >  2:708580/1‑139 (MQ=255)

                      cATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGAtt                                                                                                        >  2:5526/1‑139 (MQ=255)

                        tGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTgc                                                                                                      >  2:636790/1‑139 (MQ=255)

                                ttCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCaaaa                                                                                              <  1:143478/139‑1 (MQ=255)

                                      aaaGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCaaa                                                                                                                                <  1:147993/99‑1 (MQ=255)

                                      aaaGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCaaa                                                                                                                                >  2:147993/1‑99 (MQ=255)

                                          cgcCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATat                                                                                    >  2:397845/1‑138 (MQ=255)

                                             ccTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGa                                                                                 >  1:60660/1‑139 (MQ=255)

                                             ccTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGa                                                                                 >  1:621614/1‑139 (MQ=255)

                                                       cAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATATTGACGCAAAACAg                                                                       <  1:397845/139‑1 (MQ=255)

                                                       cAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAg                                                                       >  2:874035/1‑139 (MQ=255)

                                                       cAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAg                                                                       >  1:451383/1‑139 (MQ=255)

                                                         aaTGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCg                                                                     >  2:653930/1‑139 (MQ=255)

                                                                          ggAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCaa                                                    <  2:621614/139‑1 (MQ=255)

                                                                                tttGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTg                                              >  2:727880/1‑139 (MQ=255)

                                                                                 ttGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGc                                             <  1:5526/139‑1 (MQ=255)

                                                                                       aaCTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGt                                       <  1:653930/139‑1 (MQ=255)

                                                                                                 aGCGCGGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAgcgc                             >  2:1007395/1‑139 (MQ=255)

                                                                                                    gcgGCACGATGCCTCCCGCAATAGACACGCTACCACATGGCCCGAAATTGAGCGCCAGATAGCCGCCAAAACGGCCCATAATGCCGCAAAACAGCGACAATGCGCGACGGCAATGGGTTCAGCTGTCAGCCAGcgcgcg                          <  2:768473/139‑1 (MQ=255)

                                                                                                    gcgGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGcgcgcg                          <  1:1007395/139‑1 (MQ=255)

                                                                                                     cgGCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGt                         <  2:60660/139‑1 (MQ=255)

                                                                                                      ggCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGtt                        <  1:636790/139‑1 (MQ=255)

                                                                                                      ggCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGtt                        <  1:274662/139‑1 (MQ=255)

                                                                                                       gCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAAt                                                   >  2:1075832/1‑111 (MQ=255)

                                                                                                       gCACGATACCGCCCGCAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAAt                                                   <  1:1075832/111‑1 (MQ=255)

                                                                                                                  cccgcAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGAtt                                                                                                        <  2:149712/47‑1 (MQ=255)

                                                                                                                  cccgcAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGAtt                                                                                                        >  1:149712/1‑47 (MQ=255)

                                                                                                                    cgcAATAAACACGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGAGCGGCGGCAAACGGTGCAGCTGTCAGTCAGCGCGCGTTCGGTAATATCtttt          >  2:82134/1‑139 (MQ=255)

                                                                                                                            acacGCCACCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGTTCGGTAATATCTTTTGGCTTgag  <  1:727880/139‑1 (MQ=255)

ACGATGAGATACACCGGAATATCATGGACATATTCTTTAAAGCGCCCTTTATCTTCAAATGCGGCACGGAAACCGGAGGCTTTGAAGAACTCAAGGAAGCGCGGCACGATACCGCCCGCAATAAACACGCCGCCAAATGTCCCGAGATTGAGCGCCAGATTGCCGCCAAAACGGCCCATAATGACGCAAAACAGCGACAATGCGCGGCGGCAATCGGTGCAGCTGTCAGCCAGCGCGCGTTCGGTAATATCTTTTGGCTTGAG  >  NC_000913/2508528‑2508790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A