Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,608,141 |
A→T |
T409S (ACC→TCC) |
hyfF → |
hydrogenase 4, membrane subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,608,141 | 0 | A | T | 100.0%
| 90.0
/ NA
| 29 | T409S (ACC→TCC) | hyfF | hydrogenase 4, membrane subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (14/15); total (14/15) |
GCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGC > NC_000913/2608017‑2608221
|
gcgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGa < 2:279646/139‑1 (MQ=255)
cgcgATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGAc > 1:1003542/1‑139 (MQ=255)
gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAAt > 2:442867/1‑135 (MQ=255)
gtcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAAt < 1:442867/135‑1 (MQ=255)
tcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCTCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACct < 1:805227/139‑1 (MQ=255)
tcTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACct > 2:175702/1‑139 (MQ=255)
cTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACctg < 1:175702/139‑1 (MQ=255)
gCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGc < 1:243292/117‑1 (MQ=255)
gCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGc > 2:243292/1‑117 (MQ=255)
gCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATc > 1:1049804/1‑139 (MQ=255)
gCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATc > 2:133039/1‑139 (MQ=255)
cTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCg > 1:1084693/1‑139 (MQ=255)
aaaaTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGc > 1:673112/1‑113 (MQ=255)
aaaaTCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGc < 2:673112/113‑1 (MQ=255)
aTTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTg < 2:1003542/139‑1 (MQ=255)
gTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTcc < 1:796924/118‑1 (MQ=255)
gTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTcc > 2:796924/1‑118 (MQ=255)
gTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGc < 2:16318/139‑1 (MQ=255)
tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgc < 1:458305/120‑1 (MQ=255)
tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCctgc > 2:458305/1‑120 (MQ=255)
tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTg < 2:726215/128‑1 (MQ=255)
tGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTg > 1:726215/1‑128 (MQ=255)
cggcggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGTCGGACTGGc > 2:1072565/1‑81 (MQ=255)
cggcggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGTCGGACTGGc < 1:1072565/81‑1 (MQ=255)
cggcggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTc < 2:979205/106‑1 (MQ=255)
cggcggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTc > 1:979205/1‑106 (MQ=255)
cggTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGc < 1:133039/139‑1 (MQ=255)
aGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCct < 2:197287/59‑1 (MQ=255)
aGCGAATTTATGTCCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCct > 1:197287/1‑59 (MQ=255)
|
GCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAATCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGCGAATTTATGACCATTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGTGCTGGCGGGC > NC_000913/2608017‑2608221
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
