Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F1 I2 R1 | 765 | 46.1 | 2269152 | 93.8% | 2128464 | 105.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,724,448 | C→T | H107H (CAC→CAT) *433* (TAG→TAA) |
yfiM → kgtP ← |
putative lipoprotein alpha‑ketoglutarate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,724,448 | 0 | C | T | 100.0% | 57.2 / NA | 19 | H107H (CAC→CAT) *433* (TAG→TAA) | yfiM kgtP | putative lipoprotein alpha‑ketoglutarate transporter |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (10/9); total (10/9) | |||||||||||
TGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCG > NC_000913/2724319‑2724581 | tGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc < 2:633352/139‑1 (MQ=255) ggCGTCAAAAGAGCTTTGGGATCGCCTCACCGAAGGGAGCGGCTGGAGCTGGATGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCcccttccct > 2:981845/1‑139 (MQ=255) cTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc < 2:1009171/114‑1 (MQ=255) cTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGc > 1:1009171/1‑114 (MQ=255) aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg < 1:147713/110‑1 (MQ=255) aGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATg > 2:147713/1‑110 (MQ=255) ggATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTcaca < 2:1081147/139‑1 (MQ=255) ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATcc < 2:744440/73‑1 (MQ=255) ttGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATcc > 1:744440/1‑73 (MQ=255) ggATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGaagaag > 2:1039488/1‑139 (MQ=255) tCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGt > 2:825450/1‑139 (MQ=255) ccGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTc < 1:141775/139‑1 (MQ=255) gCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaaa < 1:947481/82‑1 (MQ=255) gCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGaaa > 2:947481/1‑82 (MQ=255) gCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTc < 1:1039488/139‑1 (MQ=255) taCCGTCTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGc > 1:443693/1‑139 (MQ=255) cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaag > 1:57051/1‑103 (MQ=255) cTGGCAACTGACCCGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCagaag < 2:57051/103‑1 (MQ=255) cGTCATTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTccg > 2:125835/1‑139 (MQ=255) | TGTCAGTTTGGGGGCGTCAAAAGAGCTTTGGGATAGCCGCCCCGAAGGGAGCGGCTGGAGCTGGAAGGATTTGGCCTGGGATGTCGCCGGTGCAAGCACCGGCTATACCGTCTGGCAACTGACCCGTCACTAAAGACGCATCCCCTTCCCTTTGCGATGTAGCATCAAAGAAACCAGAAACGCCACCACGGCCATCAAGGTCACATACCAGAAGAAGGCTGTTTCCATTCCTATTGATTTCAGCGACAACGCTACGTACTCCG > NC_000913/2724319‑2724581 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |