Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A1 F1 I2 R1 | 765 | 46.1 | 2269152 | 93.8% | 2128464 | 105.7 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,765,829 | (C)5→4 | intergenic (‑53/‑89) | yfjM ← / → rnlA | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,765,825 | 0 | C | . | 100.0% | 206.3 / NA | 49 | intergenic (‑49/‑93) | yfjM/rnlA | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, RNase LS |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (26/23); total (26/23) | |||||||||||
TGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACT > NC_000913/2765689‑2765942 | tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGccc > 1:232677/1‑139 (MQ=255) aataatTACTAAGTATCGAGTTGCTGGTAGGTGAGATCAGGAGGAGGTTACCGAAACGTTGGACATCAT‑CCCCCTATACGTGGACTGCCCCCCCGGTAAACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTgg > 2:136493/1‑139 (MQ=255) aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTgg < 2:232677/139‑1 (MQ=255) aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTgg > 1:421010/1‑139 (MQ=255) tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATac < 2:421010/139‑1 (MQ=255) ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATacac > 1:82137/1‑139 (MQ=255) gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 2:421495/139‑1 (MQ=255) tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCaa > 1:662502/1‑126 (MQ=255) tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCaa < 2:662502/126‑1 (MQ=255) gAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTCTTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTattatt > 2:817118/1‑139 (MQ=255) gAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTattatt > 1:107260/1‑139 (MQ=255) gagGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTa < 1:817118/139‑1 (MQ=255) ggTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga > 2:1085621/1‑139 (MQ=255) ggTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTaga > 2:68156/1‑139 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCa > 1:1002450/1‑89 (MQ=255) gTTACCGAAACGATGGACATCATCCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCa < 2:1002450/89‑1 (MQ=255) tACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta > 1:569087/1‑126 (MQ=255) tACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta < 2:569087/126‑1 (MQ=255) cGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc > 1:24608/1‑139 (MQ=255) aaaCGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcccc < 2:386168/139‑1 (MQ=255) aCGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCat < 2:107260/139‑1 (MQ=255) aTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc > 1:780501/1‑51 (MQ=255) aTTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGc < 2:780501/51‑1 (MQ=255) tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa < 2:24608/139‑1 (MQ=255) tgtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa < 1:1085621/139‑1 (MQ=255) tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca < 2:120711/76‑1 (MQ=255) tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATaca > 1:120711/1‑76 (MQ=255) tGCCACCCCGGTAGACGATGCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGATGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt > 2:843476/1‑139 (MQ=255) gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa > 2:476548/1‑73 (MQ=255) gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa > 2:293893/1‑73 (MQ=255) gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa < 1:293893/73‑1 (MQ=255) gCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa < 1:476548/73‑1 (MQ=255) ccGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGt > 1:656708/1‑139 (MQ=255) gTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg > 1:710801/1‑72 (MQ=255) gTAGACGATCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAg < 2:710801/72‑1 (MQ=255) aTCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTGCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt < 2:656708/139‑1 (MQ=255) aTCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt > 1:572512/1‑139 (MQ=255) aTCCTGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt > 1:1082068/1‑139 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 2:542680/66‑1 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg > 1:542680/1‑66 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg > 1:501931/1‑63 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg < 2:501931/63‑1 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc > 2:956501/1‑104 (MQ=255) tGCCCTATAGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc < 1:956501/104‑1 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 2:740005/59‑1 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg > 1:864662/1‑59 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg < 2:864662/59‑1 (MQ=255) taGTTGGAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg > 1:740005/1‑59 (MQ=255) ggAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa < 1:14957/41‑1 (MQ=255) ggAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATa > 2:14957/1‑41 (MQ=255) ggAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa > 2:651691/1‑75 (MQ=255) ggAGTG‑CCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa < 1:651691/75‑1 (MQ=255) | TGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCAT‑CCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACT > NC_000913/2765689‑2765942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |