Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
765 |
46.1 |
2269152 |
93.8% |
2128464 |
105.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,098,543 |
T→C |
intergenic (+140/+15) |
yggW → / ← yggM |
HemN family putative oxidoreductase/DUF1202 family putative secreted protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,098,543 | 0 | T | C | 100.0%
| 98.9
/ NA
| 29 | intergenic (+140/+15) | yggW/yggM | HemN family putative oxidoreductase/DUF1202 family putative secreted protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/17); total (12/17) |
GTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTT > NC_000913/3098408‑3098657
|
gTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 2:817117/139‑1 (MQ=255)
tATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCAc < 1:123135/139‑1 (MQ=255)
tATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCAc < 1:258141/139‑1 (MQ=255)
tATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCAc < 1:789876/139‑1 (MQ=255)
aTGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCaaa < 1:609987/139‑1 (MQ=255)
aTCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCaaa < 2:724152/121‑1 (MQ=255)
aTCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCaaa > 1:724152/1‑121 (MQ=255)
aTGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa > 2:786510/1‑100 (MQ=255)
aTGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 1:786510/100‑1 (MQ=255)
cAGCGGTTGTAGGTCTGTTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCg < 2:1126161/139‑1 (MQ=255)
gATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGc > 1:381145/1‑139 (MQ=255)
aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa > 2:490553/1‑77 (MQ=255)
aTAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 1:490553/77‑1 (MQ=255)
gACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa > 1:1086612/1‑73 (MQ=255)
gACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 2:1086612/73‑1 (MQ=255)
aCGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCaaa > 1:890214/1‑82 (MQ=255)
aCGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCaaa < 2:890214/82‑1 (MQ=255)
cgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 2:291122/69‑1 (MQ=255)
cgcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa > 1:291122/1‑69 (MQ=255)
gcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCGGCGTTCATTAGc < 1:1073157/139‑1 (MQ=255)
gcAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGc < 2:381145/139‑1 (MQ=255)
tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa > 2:441641/1‑56 (MQ=255)
tCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCa < 1:441641/56‑1 (MQ=255)
cGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCa < 1:664810/78‑1 (MQ=255)
cGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCa > 2:664810/1‑78 (MQ=255)
gTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAAt > 1:436240/1‑139 (MQ=255)
gATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAactta > 1:516494/1‑61 (MQ=255)
gATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGCGCACTATATCAAactta < 2:516494/61‑1 (MQ=255)
aaCGCCGCATCCTGCTACAAATCGCGCACTATATCAAACTTACTTATTCACCCCATTGCGCCGCAGCTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACtttt > 2:827267/1‑139 (MQ=255)
|
GTATTGCCGGATGCGGCGTGAACGCCTTATCCAGCCGACATGTGGCAGCGGTTGTAGGTCTGATAAGACGCGCAAGCGTCGCATCAGACGTTGATTGCCGGATGCGGGGTCAACGCCGCATCCTGCTACAAATCGTGCACTATATCAAACTTACTTAATCACCCCATTGCGCCGCAGTTGTTCGGTATCTTTTTTCCAGCGTTCATTAGCTTGGTTGATATCGTCAATTTTCATCAAAATCGCGACTTTT > NC_000913/3098408‑3098657
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
