Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,187,153	A→G	L363P (CTG→CCG)	roxA ←	50S ribosomal protein L16 arginine hydroxylase, 2‑oxoglutarate oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,187,153	0	A	G	100.0%	171.7 / NA	48	L363P (CTG→CCG)	roxA	50S ribosomal protein L16 arginine hydroxylase, 2‑oxoglutarate oxygenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/27); total (21/27)

GCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATACA  >  NC_000913/1187020‑1187288

gCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGcgcc                                                                                                                                    >  1:927504/1‑139 (MQ=255)

 cGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGcgccg                                                                                                                                   <  2:131169/139‑1 (MQ=255)

     gCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAg                                                                                                                               <  2:927504/139‑1 (MQ=255)

       aGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCa                                                                                                                             <  2:395248/139‑1 (MQ=255)

              cGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAg                                                                                                                      >  2:1650599/1‑139 (MQ=255)

                tCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGc                                                                                                                              <  2:970250/129‑1 (MQ=255)

                tCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGc                                                                                                                              >  1:970250/1‑129 (MQ=255)

                tCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGa                                                                                                                    >  1:308408/1‑139 (MQ=255)

                   gTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATg                                                                                                                 <  1:1549190/139‑1 (MQ=255)

                       tGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACgg                                                                                                             >  2:615154/1‑139 (MQ=255)

                        gCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGAGAGCATCGCGAGGAATGACGGa                                                                                                            <  1:324911/139‑1 (MQ=255)

                           gAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCt                                                                                                         >  1:164276/1‑139 (MQ=255)

                             gTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTc                                                                                                       >  2:942072/1‑139 (MQ=255)

                                        aaCGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCcgcg                                                                                                                                 <  2:390598/102‑1 (MQ=255)

                                        aaCGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCcgcg                                                                                                                                 >  1:390598/1‑102 (MQ=255)

                                                       aaaGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAg                                                                             >  2:724163/1‑139 (MQ=255)

                                                                cGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCaa                                                                    >  1:959471/1‑139 (MQ=255)

                                                                         aTGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGt                                                                            <  1:658459/122‑1 (MQ=255)

                                                                         aTGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGt                                                                            >  2:658459/1‑122 (MQ=255)

                                                                           gCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCg                                                         >  1:877333/1‑139 (MQ=255)

                                                                            ccGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGa                                                        <  1:615154/139‑1 (MQ=255)

                                                                               cATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATcgcg                                                                                                                        >  1:286216/1‑72 (MQ=255)

                                                                               cATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATcgcg                                                                                                                        <  2:286216/72‑1 (MQ=255)

                                                                                aTCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGc                                                    >  2:380984/1‑139 (MQ=255)

                                                                                 tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                           <  2:688738/131‑1 (MQ=255)

                                                                                 tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTgg                                                           >  1:688738/1‑131 (MQ=255)

                                                                                 tCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCa                                                   <  2:373620/139‑1 (MQ=255)

                                                                                   cGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATc                                                 <  1:1188240/139‑1 (MQ=255)

                                                                                   cGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATc                                                 <  2:877333/139‑1 (MQ=255)

                                                                                             ccAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGa                                       <  1:506665/139‑1 (MQ=255)

                                                                                             ccAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGa                                       <  2:1330174/139‑1 (MQ=255)

                                                                                             ccAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGa                                       <  1:25406/139‑1 (MQ=255)

                                                                                             ccAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGa                                       <  2:308408/139‑1 (MQ=255)

                                                                                              cAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGAc                                      <  2:334124/139‑1 (MQ=255)

                                                                                                 aCTTACCCTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGt                                   <  2:1073698/139‑1 (MQ=255)

                                                                                                       ccTTCGAAGAACCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCgggg                             >  1:1457819/1‑139 (MQ=255)

                                                                                                            gaagaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAg                                                                         <  2:1613393/90‑1 (MQ=255)

                                                                                                            gaagaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAg                                                                         >  1:1613393/1‑90 (MQ=255)

                                                                                                             aagaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCa                                                                              <  1:939125/84‑1 (MQ=255)

                                                                                                             aagaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCa                                                                              >  2:939125/1‑84 (MQ=255)

                                                                                                             aagaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGa                       >  1:1660548/1‑139 (MQ=255)

                                                                                                              agaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAt                      <  1:1650599/139‑1 (MQ=255)

                                                                                                               gaaCCAATACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGAtc                     <  1:724163/139‑1 (MQ=255)

                                                                                                                     aTACCCGCTATTTACCGGCACCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGTGGGGGATCGATCTTCTCa               >  2:298777/1‑139 (MQ=255)

                                                                                                                      tACCCGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCAc              <  2:1660548/139‑1 (MQ=255)

                                                                                                                        cccGCTATTGACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCa            <  1:942072/139‑1 (MQ=255)

                                                                                                                                 gACCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATac   <  2:45490/139‑1 (MQ=255)

                                                                                                                                  aCCGGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATaca  >  1:903478/1‑139 (MQ=255)

GCGGTGCAGGTGATCGTCCGTATTGCCGAGTTAACGGCGCAACGGAAGTAAGCGAAAAGGGATGCGGCATGTGATGCCGCATCCGGCTTAAATCCAAACTTACCCTTCGAAGAACCAATACCCGCTATTGACCAGCGCCGCGAGCATCGCGAGGAATGACGGATCTTCCAGCGCATCGCCAAAATTCTCCGCAGTCAGCGCAATGTTGCTGGCGAGTGCATCCAGTGCCGGACGGTGCGGGGAATCGATCTTCTCACCATTGGCATACA  >  NC_000913/1187020‑1187288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A