Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,450,392	C→T	A320T (GCA→ACA)	tynA ←	tyramine oxidase, copper‑requiring

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,450,392	0	C	T	100.0%	120.6 / NA	39	A320T (GCA→ACA)	tynA	tyramine oxidase, copper‑requiring
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (20/19); total (20/19)

GCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGA  >  NC_000913/1450261‑1450526

gCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCAtt                                                                                                                                 <  1:614388/139‑1 (MQ=255)

        gAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCgg                                                                                                                         >  1:1476214/1‑139 (MQ=255)

                cAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCg                                                                                                                 >  1:590063/1‑139 (MQ=255)

                   ttCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGCTCATTGGCACCGGAACTACCGGAc                                                                                                              <  2:1121209/139‑1 (MQ=255)

                                          aGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                                             >  2:771140/1‑117 (MQ=255)

                                          aGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                                             <  1:771140/117‑1 (MQ=255)

                                          aGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGAttttt                                                                                       >  1:1023006/1‑139 (MQ=255)

                                            taatcgctaactttttGCATTCAGGTTTCATGATTTGCACAAGCCTAATCGCCGGAGCAACGCGGTAACTGCCATTAAATGGGTGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTAAATCTTAACGAttttttt                                                                                     <  2:802336/128‑1 (MQ=255)

                                               tGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTg                                                                                  >  2:520463/1‑139 (MQ=255)

                                                     aaTTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACCACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTaa                                                                            >  1:791966/1‑139 (MQ=255)

                                                           tACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTCAACGATTTTTTTCTGTTCTAAATCAAc                                                                      >  1:1130277/1‑139 (MQ=255)

                                                              cTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGAc                                                                   <  1:372014/139‑1 (MQ=255)

                                                                    gCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGccac                                                             >  2:943897/1‑139 (MQ=255)

                                                                       cAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAg                                                          >  1:776676/1‑139 (MQ=255)

                                                                           gATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTGACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                                      <  2:729160/139‑1 (MQ=255)

                                                                           gATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGttt                                                      <  1:520463/139‑1 (MQ=255)

                                                                                  aTAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCtt                                                                                                 <  1:718657/89‑1 (MQ=255)

                                                                                  aTAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCtt                                                                                                 >  2:718657/1‑89 (MQ=255)

                                                                                   tAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATggg                                              <  2:1534856/139‑1 (MQ=255)

                                                                                      gCTTAACTGCCGGAGCAACGCGGTCTCGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGAtg                                           >  2:582749/1‑139 (MQ=255)

                                                                                          aaCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                       <  1:943897/139‑1 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                                             <  1:854032/68‑1 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAcc                                                                                                             >  2:854032/1‑68 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGAc                                                                                                              >  1:974890/1‑67 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGt                                                                                 <  2:712185/96‑1 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGt                                                                                 >  1:712185/1‑96 (MQ=255)

                                                                                           aCTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCACTGGCACCGGAACTACCGGAc                                                                                                              <  2:974890/67‑1 (MQ=255)

                                                                                                cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                       <  2:123961/133‑1 (MQ=255)

                                                                                                cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGcc                                       >  1:123961/1‑133 (MQ=255)

                                                                                                cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtag                                 >  2:407645/1‑139 (MQ=255)

                                                                                                cGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCagtag                                 >  1:756977/1‑139 (MQ=255)

                                                                                                       aCGCGGTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCAt                          <  2:1528200/139‑1 (MQ=255)

                                                                                                           ggTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCAcc                      <  2:1059137/139‑1 (MQ=255)

                                                                                                           ggTCACGGCCATCAAATGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCAcc                      <  2:791966/139‑1 (MQ=255)

                                                                                                                         aaTGGGCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGc        <  1:407645/139‑1 (MQ=255)

                                                                                                                           tgagCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtg      >  2:960861/4‑139 (MQ=255)

                                                                                                                            gggCGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtga     <  2:1476214/139‑1 (MQ=255)

                                                                                                                               cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatga  >  2:138246/1‑139 (MQ=255)

                                                                                                                               cGTGTGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCtgatga  >  1:1006407/1‑139 (MQ=255)

GCTGAGGTGAAAATCCCAGTTCCGCCAGTGAATCATATCGCCAGTAATGGTGTAATTTTTACCTTCAGGCTCAATGATTTGCATAGGCTTAACTGCCGGAGCAACGCGGTCACGGCCATCAAATGGGCGTGCGGTCATTGGCACCGGAACTACCGGACCTTCTTCAATCTTAACGATTTTTTTCTGTTCTAAATCAACGACCGCCACCAGGTTTTCGATGGGATGTGCCCAGTAGTTGCCATCACCGACATCAAGATAGCTGATGA  >  NC_000913/1450261‑1450526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A