Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	760	67.6	3323148	92.7%	3080558	109.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,056,894	(A)_7→6	coding (37/717 nt)	yeeN →	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,056,888	0	A	.	100.0%	295.5 / NA	72	coding (31/717 nt)	yeeN	UPF0082 family protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (39/33); total (39/33)

CTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGC  >  NC_000913/2056758‑2057018

cTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaaacgg                                                                                                                            <  2:1152560/139‑4 (MQ=255)

       aCGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGa                                                                                                                    >  2:329398/1‑139 (MQ=255)

              ttttCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCa                                                                                                             <  1:168255/139‑1 (MQ=255)

                             ataataTGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTAt                                                                                              <  1:329398/139‑1 (MQ=255)

                                atatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCa                                                                                           >  2:1480182/1‑139 (MQ=255)

                                 tatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaa                                                                                          >  2:800910/1‑139 (MQ=255)

                                    gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCaa                                                                                                            >  1:874057/1‑118 (MQ=255)

                                    gCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCaa                                                                                                            <  2:874057/118‑1 (MQ=255)

                                     cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCt                                                                                                                         >  2:971642/1‑104 (MQ=255)

                                     cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCt                                                                                                                         <  1:935192/104‑1 (MQ=255)

                                     cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCt                                                                                                                         <  1:971642/104‑1 (MQ=255)

                                     cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCt                                                                                                                         >  2:935192/1‑104 (MQ=255)

                                      tGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTc                                                                                     >  2:452003/1‑139 (MQ=255)

                                      tGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTc                                                                                     >  2:1569657/1‑139 (MQ=255)

                                      tGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTc                                                                                     >  1:34898/1‑139 (MQ=255)

                                        aaTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                                                                                   >  2:342319/1‑139 (MQ=255)

                                        aaTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCgg                                                                                   >  2:837450/1‑139 (MQ=255)

                                               acaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGaa                                                                            <  1:1569657/139‑1 (MQ=255)

                                                       aCGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgc                                                                    <  1:1352426/139‑1 (MQ=255)

                                                       aCGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgc                                                                    <  1:995235/139‑1 (MQ=255)

                                                       aCGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgc                                                                    <  1:452003/139‑1 (MQ=255)

                                                        cGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgct                                                                   <  2:34898/139‑1 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaaa                                                                                                                               <  2:506680/75‑1 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaaa                                                                                                                               >  1:506680/1‑75 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCa                                                                                                             <  2:1408997/92‑1 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCa                                                                                                             <  1:612097/92‑1 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCa                                                                                                             >  2:612097/1‑92 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCa                                                                                                             >  1:1408997/1‑92 (MQ=255)

                                                             tACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctgc                                                              >  2:705890/1‑139 (MQ=255)

                                                                     aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaa                                                                                                                                <  1:97590/66‑1 (MQ=255)

                                                                     aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaa                                                                                                                                >  2:97590/1‑66 (MQ=255)

                                                                     aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAg                                                      >  2:1615474/1‑139 (MQ=255)

                                                                     aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAg                                                      >  2:1245586/1‑139 (MQ=255)

                                                                     aTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCT‑CTGCTAAACAAgg                                                     >  1:231000/1‑139 (MQ=255)

                                                                          aataaatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGaa                                                 >  1:277665/1‑139 (MQ=255)

                                                                            taaatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                               <  1:1615474/139‑1 (MQ=255)

                                                                             aaatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAccc                                              >  2:718556/1‑139 (MQ=255)

                                                                                ttttCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCT‑CTGCTAAACAAGGTGAACCCGATc                                          <  2:231000/139‑1 (MQ=255)

                                                                                 tttCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATc                                          <  1:837450/139‑1 (MQ=255)

                                                                                  ttCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATcc                                         >  1:1337848/1‑139 (MQ=255)

                                                                                   tCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCa                                        <  2:1337848/139‑1 (MQ=255)

                                                                                      aggagATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGaa                                     <  1:705890/139‑1 (MQ=255)

                                                                                           aTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctgc                                                              >  1:222191/1‑109 (MQ=255)

                                                                                           aTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctgc                                                              <  2:222191/109‑1 (MQ=255)

                                                                                             cATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAaca                              <  2:277665/139‑1 (MQ=255)

                                                                                             cATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAaca                              <  1:342319/139‑1 (MQ=255)

                                                                                              aTTCCAGTGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAacac                             <  1:800910/139‑1 (MQ=255)

                                                                                                     tGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGGTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                               <  2:252352/114‑1 (MQ=255)

                                                                                                     tGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTTTGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTa                      >  1:1110745/1‑139 (MQ=255)

                                                                                                     tGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTa                      >  2:520256/1‑139 (MQ=255)

                                                                                                     tGGGACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATATATGCTGCTGCTAAACAAGGTGAAcc                                               >  1:252352/1‑114 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTaaa                                                          >  2:888570/1‑101 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTaaa                                                          >  1:1036359/1‑101 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTaaa                                                          <  2:1036359/101‑1 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTaaa                                                          <  1:888570/101‑1 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                               <  2:175265/112‑1 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                               >  1:175265/1‑112 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTaaa                    >  1:888386/1‑139 (MQ=255)

                                                                                                       ggACGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTaaa                    >  2:419050/1‑139 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTa                                                                       >  2:1364761/1‑85 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTa                                                                       <  1:1364761/85‑1 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTa                                                                       <  1:293688/85‑1 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTa                                                                       >  2:293688/1‑85 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctg                                                               >  1:1135371/1‑93 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgctg                                                               <  2:1135371/93‑1 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGCATTAAACACATCTTTAAAAtt                 >  1:974051/1‑139 (MQ=255)

                                                                                                          cGTAAATGGGCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAAtt                 >  1:1421961/1‑139 (MQ=255)

                                                                                                                  ggCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAg                                                      >  2:384851/1‑94 (MQ=255)

                                                                                                                  ggCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAg                                                      <  1:384851/94‑1 (MQ=255)

                                                                                                                  ggCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTaaa                                <  2:702175/116‑1 (MQ=255)

                                                                                                                  ggCCAATATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTaaa                                >  1:702175/1‑116 (MQ=255)

                                                                                                                      aaTATTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACg     >  2:1511280/1‑139 (MQ=255)

                                                                                                                         atTGTTGCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGc  <  1:419050/139‑1 (MQ=255)

                                                                                                                             ttgCT‑AAAAAACGGCTAATGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAG‑AATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCa                                        >  2:921079/1‑96 (MQ=255)

                                                                                                                             ttgCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAacac                             >  2:782864/1‑108 (MQ=255)

                                                                                                                             ttgCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAacac                             <  1:782864/108‑1 (MQ=255)

                                                                                                                             ttgCT‑AAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAG‑AATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCa                                        <  1:921079/96‑1 (MQ=255)

CTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGC  >  NC_000913/2056758‑2057018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A