Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F1 I2 R1 760 67.6 3323148 92.7% 3080558 109.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,068,952 C→T *302* (TAG→TAA)  insD1 ← IS2 transposase TnpB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,068,9520CT99.2% 1304.0 / ‑3.6 402*302* (TAG→TAA) insD1IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (197/201);  total (199/203)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 402 total aligned reads displayed.

TTATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT  >  NC_000913/2068816‑2069073
                                                                                                                                        |                                                                                                                         
  aTTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtata                                                                                                                       >  2:944721/1‑139 (MQ=255)
  aTTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtata                                                                                                                       >  2:1423135/1‑139 (MQ=255)
    tGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAtt                                                                                                                     >  1:648899/1‑139 (MQ=255)
     gACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAttt                                                                                                                    <  2:524502/139‑1 (MQ=255)
         aacaaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAg                                                                                                                >  2:1438296/1‑139 (MQ=255)
          acaaacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                               >  1:514699/1‑139 (MQ=255)
             aacaTTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                            <  2:1617270/139‑1 (MQ=255)
                  tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                       <  2:1517164/139‑1 (MQ=255)
                  tGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                       <  2:54862/139‑1 (MQ=255)
                        aCTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 <  2:1635729/139‑1 (MQ=255)
                        aCTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 <  1:806804/139‑1 (MQ=255)
                           ccaccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTaa                                                                                              <  2:1362923/139‑1 (MQ=255)
                            caccaATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                      >  1:1424556/1‑130 (MQ=255)
                                 aTGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                             >  1:614840/1‑118 (MQ=255)
                                 aTGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACGCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                             <  2:614840/118‑1 (MQ=255)
                                   gCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                      >  1:877477/1‑123 (MQ=255)
                                    ccTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACa                                                                                     >  2:474564/1‑139 (MQ=255)
                                      tCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat                                                                                                                        >  1:1396802/1‑102 (MQ=255)
                                      tCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat                                                                                                                        <  2:923070/102‑1 (MQ=255)
                                        cATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                 >  1:415944/1‑139 (MQ=255)
                                        cATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                 >  2:1385352/1‑139 (MQ=255)
                                          tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  2:1255762/1‑139 (MQ=255)
                                          tAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               <  2:621168/139‑1 (MQ=255)
                                           aGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                            >  2:558384/1‑125 (MQ=255)
                                             ccGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTg                                                                            >  2:1574314/1‑139 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAttt                                                                                                                    <  1:669307/96‑1 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                       <  1:1458029/109‑1 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                            >  2:589350/1‑120 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  2:771767/1‑133 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                         <  1:1541821/139‑1 (MQ=255)
                                                aTAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                         <  2:223875/139‑1 (MQ=255)
                                                      tGCAGTACATCCCCGGAGTAAAGATAACAGACAGCTCCTTTGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTCATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGata                                                                   >  2:379519/1‑139 (MQ=25)
                                                      tGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGata                                                                   >  1:1558598/1‑139 (MQ=255)
                                                       gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 >  1:1134741/1‑108 (MQ=255)
                                                       gCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                 <  2:1134741/108‑1 (MQ=255)
                                                              aTCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCa                                                                                                                 <  2:438549/85‑1 (MQ=255)
                                                              aTCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACa                                                                                                             >  1:1580969/1‑89 (MQ=255)
                                                              aTCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                            >  1:1601171/1‑90 (MQ=255)
                                                                ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTc                                                                                                                   <  2:1534650/81‑1 (MQ=255)
                                                                ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  1:105341/1‑117 (MQ=255)
                                                                ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                         >  1:1640298/1‑139 (MQ=255)
                                                                ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                         >  2:1335861/1‑139 (MQ=255)
                                                                ccccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgc                                                         >  1:579636/1‑139 (MQ=255)
                                                                 cccGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                        <  1:278026/139‑1 (MQ=255)
                                                                   cGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgag                                                      <  1:951549/139‑1 (MQ=255)
                                                                      aGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  1:240391/1‑111 (MQ=255)
                                                                      aGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               <  2:240391/111‑1 (MQ=255)
                                                                        tAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                 >  2:3508/1‑139 (MQ=255)
                                                                          gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTc                                                                                                                   >  1:1614525/1‑71 (MQ=255)
                                                                          gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                            <  2:898526/78‑1 (MQ=255)
                                                                          gagaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                            <  2:264838/94‑1 (MQ=255)
                                                                           agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCg                                                                         >  2:835984/1‑112 (MQ=255)
                                                                           agaTAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAcc                                              <  2:105201/139‑1 (MQ=255)
                                                                                aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                               >  1:1532534/1‑69 (MQ=255)
                                                                                aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                               >  1:468783/1‑69 (MQ=255)
                                                                                aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         >  2:97199/1‑139 (MQ=255)
                                                                                aCAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         >  2:1603596/1‑139 (MQ=255)
                                                                                  aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgataacccagcgca                                           <  2:1592741/135‑13 (MQ=21)
                                                                                  aGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa                                       <  1:1361657/139‑1 (MQ=255)
                                                                                    aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga                                                   <  1:42911/125‑1 (MQ=255)
                                                                                    aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcgagcga                                                   >  1:931286/1‑125 (MQ=255)
                                                                                    aCAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACt                                     >  1:863000/1‑139 (MQ=255)
                                                                                       aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat                                                                                                                        >  2:1299525/1‑53 (MQ=255)
                                                                                       aCTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTtat                                                                                                                        <  1:1299525/53‑1 (MQ=255)
                                                                                        cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAttt                                                                                                                    <  1:192580/56‑1 (MQ=255)
                                                                                        cTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATAttt                                                                                                                    >  2:192580/1‑56 (MQ=255)
                                                                                           cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCTGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa                              >  1:557211/1‑139 (MQ=255)
                                                                                           cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa                              <  1:1235647/139‑1 (MQ=255)
                                                                                           cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa                              <  1:356283/139‑1 (MQ=255)
                                                                                           cTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGa                              <  2:691945/139‑1 (MQ=255)
                                                                                               gCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                          <  1:98753/139‑1 (MQ=255)
                                                                                               gCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                          <  1:1500177/139‑1 (MQ=255)
                                                                                                 aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                        <  2:1371049/139‑1 (MQ=255)
                                                                                                 aGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAt                        <  1:777125/139‑1 (MQ=255)
                                                                                                  ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGtt                                                                                                       >  2:1557839/1‑59 (MQ=255)
                                                                                                  ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                         >  1:102575/1‑137 (MQ=255)
                                                                                                  ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                         <  2:102575/137‑1 (MQ=255)
                                                                                                  ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                       >  2:420886/1‑139 (MQ=255)
                                                                                                  ggCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                       <  2:411716/139‑1 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               >  2:1206975/1‑82 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                               <  1:1206975/82‑1 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                 <  1:900478/112‑1 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                 <  1:205137/112‑1 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                          <  1:762887/135‑1 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGcc                          >  1:1365780/1‑135 (MQ=255)
                                                                                                   gCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTc                      >  1:311137/1‑139 (MQ=255)
                                                                                                    ccGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCg                     <  1:835485/139‑1 (MQ=255)
                                                                                                     cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTAt                                                                                                     <  1:406879/58‑1 (MQ=255)
                                                                                                     cGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGt                    >  2:110246/1‑139 (MQ=255)
                                                                                                     cGTAAACAAATCCGCATT‑GTGGCTGGATTTGCCCTTATATTTCCAGa                                                                                                               <  1:177959/47‑1 (MQ=255)
                                                                                                       tAAACAAATCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTta                  <  1:1335861/139‑1 (MQ=255)
                                                                                                              aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                            >  1:533175/1‑42 (MQ=255)
                                                                                                              aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                          >  1:1169799/1‑76 (MQ=255)
                                                                                                              aTCCGCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATGTTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCa                         >  2:814234/1‑125 (MQ=255)
                                                                                                              aTCCGCATTCGTGGCTGGATTTGCCCTTATACTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                          <  2:1169799/76‑1 (MQ=255)
                                                                                                                  gCATTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGaa       <  2:869576/139‑1 (MQ=38)
                                                                                                                    aTTCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                         <  2:762431/103‑1 (MQ=255)
                                                                                                                     ttCGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc    <  2:169292/139‑1 (MQ=25)
                                                                                                                       cGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct  <  2:289996/139‑1 (MQ=18)
                                                                                                                       cGTGGCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct  <  1:1102108/139‑1 (MQ=18)
                                                                                                                                        |                                                                                                                         
TTATTGACCAACAAACATTGCAGAACTCCACCAATGCCTCCATAGCCGATAATTTGCAGGACATCCCCGGAGTAGAGATAACAGACAACTCCTTGGCAGGCCGTAAACAAATCCGCATTCGTGGCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCT  >  NC_000913/2068816‑2069073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A