Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate | Predicted Mutations | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|
| A12 F1 I2 R1 | 766 | 55.6 | 2631458 | 94.7% | 2491990 | 109.9 |
Breseq alignment
| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,757,721 | C→T | *335* (TAG→TAA) | ldtE ← | murein L,D‑transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,757,721 | 0 | C | T | 100.0% | 157.4 / NA | 49 | *335* (TAG→TAA) | ldtE | murein L,D‑transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (26/23); total (26/23) | |||||||||||
CAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC > NC_000913/1757586‑1757855 | cAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTAc > 2:278780/1‑139 (MQ=255) gctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc < 2:780631/139‑1 (MQ=255) gctAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTc < 1:278780/139‑1 (MQ=255) aGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg < 2:277922/130‑1 (MQ=255) aGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg > 1:277922/1‑130 (MQ=255) cTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTcc < 2:577604/139‑1 (MQ=255) ccAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCC‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcac > 2:182414‑M1/1‑135 (MQ=255) cGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccatt > 2:399141‑M1/1‑131 (MQ=255) ggACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTG‑AAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc < 1:895690‑M1/139‑15 (MQ=255) ctactaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctact > 2:59571‑M1/1‑115 (MQ=255) actaAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactga < 1:59571‑M1/139‑27 (MQ=255) aaaTACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaagg > 1:441377‑M1/1‑110 (MQ=255) cAAGTAATAGTACTTGTAAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc < 2:776437‑M1/116‑15 (MQ=255) cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCg < 2:1169829/78‑1 (MQ=255) cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCg > 1:1169829/1‑78 (MQ=255) cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgc > 1:101331‑M1/1‑102 (MQ=255) cAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgc > 1:776437‑M1/1‑102 (MQ=255) aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcgccattctgc < 2:1296752‑M1/113‑13 (MQ=255) aaGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc > 1:1296752‑M1/1‑101 (MQ=255) aGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctg < 1:422508‑M1/139‑40 (MQ=255) ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTTGTCTGCCGTTTACCGTTACTGCg < 1:1305348/67‑1 (MQ=38) ccTGTGAAGTGAAAAATGGCGCACATTGTGCGCCATTTTTTTTTGTCTGCCGTTTACCGTTACTGCg > 2:1305348/1‑67 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg > 1:415288/1‑71 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg > 2:1185086/1‑71 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg < 1:1185086/71‑1 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCAcgcg < 2:415288/71‑1 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg > 1:400785/1‑86 (MQ=255) tgtgAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTg < 2:400785/86‑1 (MQ=255) gtgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttg > 1:1271264‑M1/1‑87 (MQ=255) gtgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttg > 2:572946‑M1/1‑87 (MQ=255) tgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct > 2:847300‑M1/1‑86 (MQ=255) tgaagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgct < 1:847300‑M1/124‑39 (MQ=255) aagtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCG‑TTACCGTTACTGCGTCCCGCGTAACATATTCCCTTGCTtcaccattctccgctgcctctactgaaggcgcattgctggttgcgggagttgctcc > 1:290840‑M1/1‑83 (MQ=255) gtgaaAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctcca < 2:441377‑M1/139‑58 (MQ=255) cgcACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccga < 1:572946‑M1/139‑69 (MQ=255) cgcACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc < 2:193914‑M1/83‑13 (MQ=255) cgcACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgc > 1:193914‑M1/1‑71 (MQ=255) cacaTTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACata > 1:866978/1‑59 (MQ=255) cacaTTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACata < 2:866978/59‑1 (MQ=255) gtgCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggat > 2:214699‑M1/1‑63 (MQ=255) gtgCGCCA‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggat < 1:399141‑M1/139‑77 (MQ=255) a‑TTTTTTTTGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtaaccattctgcgctgactctactgaaggcgctttgctggctgcgggagttgctccactgctcaccgaaaccggataccctgc > 2:252325‑M1/1‑56 (MQ=255) tttttttGTCTGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgccc > 1:1137684‑M1/1‑54 (MQ=255) tttttGTCTGCCGTTTACCGTTACTGCGTCAcgcg > 2:221848/1‑35 (MQ=255) tttttGTCTGCCGTTTACCGTTACTGCGTCAcgcg < 1:221848/35‑1 (MQ=255) tGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactcta < 2:378997‑M1/66‑23 (MQ=255) tGCCGTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactcta > 1:378997‑M1/1‑44 (MQ=255) gTTTACCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgct < 1:182414‑M1/139‑100 (MQ=255) aCCGTTACTGCGTCACGCGTAACATATTCCCTTGCTtcaccattctgcgctgactctactgaaggcgcattgctggctgcgggagttgctccactgctcaccgaaaccggataccctgcccgacgatacaacgctttat > 1:1025991‑M1/1‑36 (MQ=255) | CAGGCTGCTAAAGATGACGCAGCTCGTGCTAACCAGCGTCTGGACAACATGGCTACTAAATACCGCAAGTAATAGTACCTGTGAAGTGAAAAATGGCGCACATTGTGCGCCA‑TTTTTTTTGTCTGCCGTTTACCGCTACTGCGTCACGCGTAACATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGC > NC_000913/1757586‑1757855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |