Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,022,606	G→A	246 (TAG→TAA)	fliP →	flagellar biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,022,606	0	G	A	100.0%	138.1 / NA	43	246 (TAG→TAA)	fliP	flagellar biosynthesis protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (22/21); total (22/21)

GCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACG  >  NC_000913/2022473‑2022741

gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                    >  2:243997/1‑139 (MQ=255)

gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                    >  2:422712/1‑139 (MQ=255)

gCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAgg                                                                                                                                    >  2:57541/1‑139 (MQ=255)

 cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  1:1203999/1‑139 (MQ=255)

 cGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  2:460254/1‑139 (MQ=255)

      tGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAAt                                                                                                                              <  1:57541/139‑1 (MQ=255)

                      gatgatgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   >  1:934300/1‑118 (MQ=255)

                      gatgatgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGc                                                                                                                                   <  2:934300/118‑1 (MQ=255)

                      gatgatgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTc                                                                                                              >  1:1063046/1‑139 (MQ=255)

                           tgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           <  2:872080/137‑1 (MQ=255)

                           tgGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           >  1:872080/1‑137 (MQ=255)

                            ggTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatgatg                                                                                                        >  2:973149/1‑139 (MQ=255)

                                        caccaTTGCTCTGCCCTTTAAACTGTTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            <  2:191031/139‑1 (MQ=255)

                                        caccaTTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            <  2:1063046/139‑1 (MQ=255)

                                                   tGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           <  2:714282/113‑1 (MQ=255)

                                                   tGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           >  1:714282/1‑113 (MQ=255)

                                                                 aTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           >  2:1145257/1‑99 (MQ=255)

                                                                 aTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCatg                                                                                                           <  1:1145257/99‑1 (MQ=255)

                                                                 aTGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTgg                                                                   >  2:671716/1‑139 (MQ=255)

                                                                  tGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGc                                                                  >  1:668828/1‑139 (MQ=255)

                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            <  1:257505/108‑1 (MQ=255)

                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGaa                                                                                            >  2:257505/1‑108 (MQ=255)

                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                             <  2:182334/139‑1 (MQ=255)

                                                                       tttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGCCAAAATGACACCTGAATCGGTCATGATGATGGGGACCGAAGCGATGAAAGTCGCGCTGGCACTGGCTGccc                                                             >  2:471005/1‑139 (MQ=255)

                                                                        ttGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGcccc                                                            <  1:950930/139‑1 (MQ=255)

                                                                         tGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCg                                                           <  1:973149/139‑1 (MQ=255)

                                                                           tACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCt                                                         >  2:794643/1‑139 (MQ=255)

                                                                                gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTa                                                        >  2:14941/1‑135 (MQ=255)

                                                                                gtggATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTa                                                        <  1:14941/135‑1 (MQ=255)

                                                                                     tggctggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTa                                               >  1:226412/1‑139 (MQ=255)

                                                                                         tggcAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGt                                           <  1:460254/139‑1 (MQ=255)

                                                                                               ttGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCa                                     <  2:386925/139‑1 (MQ=255)

                                                                                                tGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCAc                                    <  2:226412/139‑1 (MQ=255)

                                                                                                  cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGTGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACgg                                  <  2:1233122/139‑1 (MQ=255)

                                                                                                  cTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACgg                                  <  1:422712/139‑1 (MQ=255)

                                                                                                       cGGTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCtt                             >  1:792445/1‑139 (MQ=255)

                                                                                                         gTTCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTat                           <  2:668828/139‑1 (MQ=255)

                                                                                                           tCGCTGGCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTatca                         >  1:999081/1‑139 (MQ=255)

                                                                                                                ggCGCAGAGCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGt                    <  2:1203999/139‑1 (MQ=255)

                                                                                                                      gagCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTg                                                                >  2:225068/1‑89 (MQ=255)

                                                                                                                      gagCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTg                                                                <  1:225068/89‑1 (MQ=255)

                                                                                                                       agCTTTTACAGCTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGc             >  1:1088201/1‑139 (MQ=255)

                                                                                                                                  cTAAAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACg  <  1:794643/139‑1 (MQ=255)

GCGTGTTGATGGCATTGGGGATGATGATGGTTCCCCCAGCCACCATTGCTCTGCCCTTTAAACTGATGCTGTTTGTACTGGTGGATGGCTGGCAATTGCTGGTCGGTTCGCTGGCGCAGAGCTTTTACAGCTAGAGAGGCAAAATGACACCTGAATCGGTCATGATGATGGGGACTGAAGCGATGAAAGTCGCGCTGGCACTGGCTGCCCCGCTATTGTTGGTAGCGTTGGTCACGGGCCTTATCATCAGTATTTTGCAGGCCGCCACG  >  NC_000913/2022473‑2022741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A