Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,8810C.94.7% 140.8 / 3.4 39intergenic (+235/‑131)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base . (15/21);  total (16/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATG  >  NC_000913/2462743‑2463001
                                                                                                                                          |                                                                                                                        
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTc                                                                                                                          >  1:1071353/1‑139 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcc                                                                                                                         <  2:547767/139‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                     <  2:184524/139‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                                                         <  2:931238/139‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                                                       <  1:696183/139‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAACCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                     <  2:373797/126‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAACCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                     >  1:373797/1‑126 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                                                       >  2:373244/1‑137 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtgtg                                                                                                       <  1:373244/137‑1 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCa                                                                                                 <  2:797324/139‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                               <  1:648483/139‑1 (MQ=255)
                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                                                         <  2:1292170/109‑1 (MQ=255)
                                              tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                                                         >  1:1292170/1‑109 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                          <  1:114872/139‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                          <  2:952582/139‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                          <  2:1212307/139‑1 (MQ=255)
                                                tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                                                         >  2:135123/1‑107 (MQ=255)
                                                tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATtg                                                                                                         <  1:135123/107‑1 (MQ=255)
                                                tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                          <  2:758569/138‑1 (MQ=255)
                                                tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                          >  1:758569/1‑138 (MQ=255)
                                                        cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                     <  1:299705/88‑1 (MQ=255)
                                                        cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccc                                                                                                                     >  2:299705/1‑88 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                                                          >  1:7703/1‑110 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                                                          <  2:7703/110‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                   <  1:779130/139‑1 (MQ=255)
                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccctgg                                                                                                                 >  2:482216/1‑74 (MQ=255)
                                                                       aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccctgg                                                                                                                 <  1:482216/77‑4 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                      <  2:1071353/139‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                     <  2:621144/139‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca                                                                                             >  2:1216419/1‑78 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATaca                                                                                             <  1:1216419/78‑1 (MQ=255)
                                                                                                gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                               >  2:500026/1‑85 (MQ=255)
                                                                                                gTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                               <  1:500026/85‑1 (MQ=255)
                                                                                                   cTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAttt                      >  2:904936/1‑139 (MQ=255)
                                                                                                     tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                             <  2:509364/98‑1 (MQ=255)
                                                                                                     tctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                             >  1:509364/1‑98 (MQ=255)
                                                                                                      ctcAAAGAGTTTACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                               >  2:950580/1‑127 (MQ=255)
                                                                                                      ctcAAAGAGTTTACTTTTCTGCATTTCCAGGATAC‑GCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTaa                               <  1:950580/127‑1 (MQ=255)
                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                >  2:544408/1‑75 (MQ=255)
                                                                                                          aaGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                <  1:544408/75‑1 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCa                                                                                                 <  1:958273/52‑1 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCa                                                                                                 >  2:958273/1‑52 (MQ=255)
                                                                                                               tttACTTTTCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa          >  2:655509/1‑139 (MQ=255)
                                                                                                                       tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                     >  2:5602/1‑104 (MQ=255)
                                                                                                                       tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                     <  1:5602/104‑1 (MQ=255)
                                                                                                                       tCTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATg  >  1:1202890/1‑139 (MQ=255)
                                                                                                                        cTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                               <  1:306077/93‑1 (MQ=255)
                                                                                                                        cTGCATTTCCAGGATACT‑CCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                               >  2:306077/1‑93 (MQ=255)
                                                                                                                                          |                                                                                                                        
TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATG  >  NC_000913/2462743‑2463001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A