Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I2 R1 766 55.6 2631458 94.7% 2491990 109.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,781,132 T→C D532G (GAC→GGC)  ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,781,1320TC100.0% 240.5 / NA 67D532G (GAC→GGC) ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/35);  total (32/35)

CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGT  >  NC_000913/2780998‑2781260
                                                                                                                                      |                                                                                                                                
cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                              >  1:105159/1‑139 (MQ=255)
cATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTg                                                                                                                              >  2:1012888/1‑139 (MQ=255)
 aTTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGa                                                                                                                             <  2:105159/139‑1 (MQ=255)
        gCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCa                                                                                                                      >  1:1237607/1‑139 (MQ=255)
          ttCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGa                                                                                                                    >  1:225894/1‑139 (MQ=255)
              gAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                                <  2:1237607/139‑1 (MQ=255)
                aTTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                              <  1:901615/139‑1 (MQ=255)
                aTTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                              <  2:107562/139‑1 (MQ=255)
                  tCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTGCCGGAGGTCGTGGAAGCCATTAATGCGCCGCCGGATTGTTCCTCAACATGCTTAGCCTCACCACCGCCCTGAGCCTCCAGAGCgccgcc                                                                                                            <  2:832242/139‑1 (MQ=255)
                        tGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCattatt                                                                                                      <  2:105909/139‑1 (MQ=255)
                                  aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGTACGCCGCCATTATTAATTCTGGTg                                                                                            <  1:803320/139‑1 (MQ=255)
                                  aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                              <  2:186143/121‑1 (MQ=255)
                                  aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccg                                                                                                              >  1:186143/1‑121 (MQ=255)
                                  aTCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTg                                                                                            <  2:535158/139‑1 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            >  1:161201/1‑121 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            <  2:161201/121‑1 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            >  2:851520/1‑121 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            <  1:851520/121‑1 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCt                                                                                          >  2:589553/1‑139 (MQ=255)
                                    cACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCt                                                                                          >  2:887360/1‑139 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccgc                                                                                                             >  2:1072269/1‑107 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgccgc                                                                                                             <  1:1072269/107‑1 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                             <  2:229486/139‑1 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                             >  2:1239453/1‑139 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                             >  2:1220611/1‑139 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                             >  2:1099385/1‑139 (MQ=255)
                                                 ggTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGcc                                                                             >  2:58162/1‑139 (MQ=255)
                                                     ccTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGttt                                                                                  >  1:1068782/1‑130 (MQ=255)
                                                     ccTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGttt                                                                                  <  2:1068782/130‑1 (MQ=255)
                                                         cGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                        <  2:912563/120‑1 (MQ=255)
                                                         cGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                        >  1:912563/1‑120 (MQ=255)
                                                                  ttCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAg                                                            >  2:351009/1‑139 (MQ=255)
                                                                    ccGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCt                                                          <  2:369235/139‑1 (MQ=255)
                                                                          gacGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGc                                                    >  2:111281/3‑139 (MQ=255)
                                                                          gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTg                                                                     >  2:267877/1‑122 (MQ=255)
                                                                          gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTg                                                                     <  1:267877/122‑1 (MQ=255)
                                                                          gTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGc                                                    >  1:1309147/1‑139 (MQ=255)
                                                                            cGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCCGGACAATCAGCTTACCGCCa                                                  >  2:252527/1‑139 (MQ=255)
                                                                                 aaGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATc                                             <  1:589553/139‑1 (MQ=255)
                                                                                    cAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcg                                                                                                                 >  1:1182462/1‑68 (MQ=255)
                                                                                    cAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcg                                                                                                                 <  2:1182462/68‑1 (MQ=255)
                                                                                     aaTTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGa                                                                   >  2:260602/1‑113 (MQ=255)
                                                                                     aaTTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGa                                                                   <  1:260602/113‑1 (MQ=255)
                                                                                        tAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGgta                                      <  2:531059/139‑1 (MQ=255)
                                                                                        tAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGgta                                      >  1:250858/1‑139 (MQ=255)
                                                                                           tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                           >  2:100491/1‑131 (MQ=255)
                                                                                           tGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCaa                                           <  1:100491/131‑1 (MQ=255)
                                                                                                 gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                                >  2:831573/1‑56 (MQ=255)
                                                                                                 gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAAcgc                                                                                                                <  1:831573/56‑1 (MQ=255)
                                                                                                 gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            >  1:528543/1‑60 (MQ=255)
                                                                                                 gccgGATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            <  2:528543/60‑1 (MQ=255)
                                                                                                     gATTGTTGCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                         <  1:887360/139‑1 (MQ=255)
                                                                                                     gATTGTTACTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCg                         <  2:263733/139‑1 (MQ=255)
                                                                                                          ttgCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGtt                    <  1:1220611/139‑1 (MQ=255)
                                                                                                            gCTCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGtttt                  <  2:250858/139‑1 (MQ=255)
                                                                                                              tCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAg                <  1:1239453/139‑1 (MQ=255)
                                                                                                              tCAACATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAg                <  2:1183031/139‑1 (MQ=255)
                                                                                                                aaCATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                        <  1:1254544/65‑1 (MQ=255)
                                                                                                                aaCATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTAt                                                                                        >  2:1254544/1‑65 (MQ=255)
                                                                                                                 aCATGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCa             <  1:241167/139‑1 (MQ=255)
                                                                                                                  cATGCTTAGCCTCACCACCGCCCTGATCCTCCAGAACGCCGCCATTATTAAGTTTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAAt                                                               <  2:850473/88‑1 (MQ=255)
                                                                                                                  cATGCTTAGCCTCACCACCGCCCTGAAGCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAAt                                                               >  1:850473/1‑88 (MQ=255)
                                                                                                                    tGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGt                                                                                                  <  1:763587/51‑1 (MQ=255)
                                                                                                                    tGCTTAGCCTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGt                                                                                                  >  2:763587/1‑51 (MQ=255)
                                                                                                                          gCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            >  2:1061943/1‑35 (MQ=255)
                                                                                                                          gCCTCACCACCGCCCTGAACCTCCAGAACgccgcc                                                                                                            <  1:1061943/35‑1 (MQ=255)
                                                                                                                            cTCACCACCGCCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGt  >  1:741827/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                
CATTTTTAGCTTCTGAATTCCTGATGTAGAAAGCATCACCATAACTGTTGGTTCCTTCGATAAGTGTTCCGGAGGTCGTGGAAGCAATTAATGCGCCGCCGGATTGTTGCTCAACATGCTTAGCCTCACCACCGTCCTGAACCTCCAGAACGCCGCCATTATTAAGTCTGGTGCTATCTGTTTTAGCCTCCTTCTGGACAATCAGCTTACCGCCAGTATCAATGGTAGTATTTTTCGCCGAGGTTTTAGCCACTACCGTCAGT  >  NC_000913/2780998‑2781260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A