Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	766	55.6	2631458	94.7%	2491990	109.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,351	G→A	230 (TAG→TAA)	mutH →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,351	0	G	A	100.0%	77.8 / NA	25	230 (TAG→TAA)	mutH	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/12); total (13/12)

CGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGC  >  NC_000913/2970224‑2970471

cGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGATGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACGATCGCttcg                                                                                                               >  2:775032/1‑137 (MQ=255)

             gcTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTc                                                                                                  <  2:1217034/139‑1 (MQ=255)

               ttACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                <  1:673932/139‑1 (MQ=255)

                       gCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                <  1:978504/131‑1 (MQ=255)

                       gCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCg                                                                                                >  2:978504/1‑131 (MQ=255)

                                   cGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                       <  1:897239/96‑1 (MQ=255)

                                   cGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCa                                                                                                                       >  2:897239/1‑96 (MQ=255)

                                      ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                             <  1:1053401/103‑1 (MQ=255)

                                      ggCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGAc                                                                                                             >  2:1053401/1‑103 (MQ=255)

                                                                     tttATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCa                                                                                            <  1:73858/89‑1 (MQ=255)

                                                                     tttATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCa                                                                                            >  2:73858/1‑89 (MQ=255)

                                                                             aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAg                                                                <  1:459002/109‑1 (MQ=255)

                                                                             aagaagaaTTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAg                                                                >  2:459002/1‑109 (MQ=255)

                                                                                                cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              >  1:428342/1‑60 (MQ=255)

                                                                                                cACTACTGGCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              <  2:428342/60‑1 (MQ=255)

                                                                                                        gCCCGTCATTTTCTGATCCAGTAATCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGa                                                     <  2:1035550/93‑1 (MQ=255)

                                                                                                        gCCCGTCATTTTCTGATCCAGTAATCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGa                                                     >  1:1035550/1‑93 (MQ=255)

                                                                                                        gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              >  1:1244807/1‑52 (MQ=255)

                                                                                                        gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              <  2:1244807/52‑1 (MQ=255)

                                                                                                        gCCCGTCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCgg       >  1:916767/1‑139 (MQ=255)

                                                                                                             tCATTTTCTGATCCAGTAACCATCTCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGtt                                                 <  1:819405/92‑1 (MQ=255)

                                                                                                             tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              <  1:971652/47‑1 (MQ=255)

                                                                                                             tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCggg                                                                                              >  2:971652/1‑47 (MQ=255)

                                                                                                             tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGtt                                                 >  2:819405/1‑92 (MQ=255)

                                                                                                             tCATTTTCTGATCCAGTAACCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgc  >  1:1071966/1‑139 (MQ=255)

CGAATGCGAAAGCGCTTACCGAAGCCATTGGTGCCCGGGGCGAACGGATTCTGACGCTGCCACGCGGCTTTTATTTGAAGAAGAATTTCACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGC  >  NC_000913/2970224‑2970471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A